Sodium in PDB 2bzd: Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.
Enzymatic activity of Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.
All present enzymatic activity of Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.:
3.2.1.18;
Protein crystallography data
The structure of Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase., PDB code: 2bzd
was solved by
S.L.Newstead,
G.Taylor,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
122.17 /
2.0
|
Space group
|
P 32 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
141.457,
141.457,
158.880,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
19.3 /
26.7
|
Sodium Binding Sites:
The binding sites of Sodium atom in the Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.
(pdb code 2bzd). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the
Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase., PDB code: 2bzd:
Jump to Sodium binding site number:
1;
2;
3;
Sodium binding site 1 out
of 3 in 2bzd
Go back to
Sodium Binding Sites List in 2bzd
Sodium binding site 1 out
of 3 in the Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1648
b:20.3
occ:1.00
|
O
|
A:ASN533
|
2.2
|
27.5
|
1.0
|
O
|
A:ALA639
|
2.3
|
18.9
|
1.0
|
O
|
A:ASN528
|
2.4
|
16.3
|
1.0
|
O
|
A:THR536
|
2.5
|
19.0
|
1.0
|
OG1
|
A:THR536
|
2.7
|
19.0
|
1.0
|
OD1
|
A:ASP531
|
2.8
|
20.7
|
1.0
|
OE2
|
A:GLU640
|
3.2
|
32.8
|
1.0
|
C
|
A:ASN533
|
3.2
|
27.1
|
1.0
|
C
|
A:THR536
|
3.4
|
19.3
|
1.0
|
C
|
A:ALA639
|
3.6
|
19.4
|
1.0
|
C
|
A:ASN528
|
3.6
|
15.5
|
1.0
|
N
|
A:ASN533
|
3.8
|
25.5
|
1.0
|
CG
|
A:ASP531
|
3.8
|
21.0
|
1.0
|
CA
|
A:ASN533
|
3.9
|
26.5
|
1.0
|
CB
|
A:THR536
|
3.9
|
19.8
|
1.0
|
CD
|
A:GLU640
|
4.0
|
30.5
|
1.0
|
CA
|
A:THR536
|
4.1
|
20.0
|
1.0
|
CG
|
A:GLU640
|
4.1
|
25.6
|
1.0
|
CB
|
A:ASN533
|
4.1
|
26.8
|
1.0
|
N
|
A:THR536
|
4.1
|
22.2
|
1.0
|
N
|
A:PRO534
|
4.2
|
27.0
|
1.0
|
OD2
|
A:ASP531
|
4.2
|
21.0
|
1.0
|
N
|
A:PHE537
|
4.3
|
18.3
|
1.0
|
CA
|
A:ALA639
|
4.4
|
19.1
|
1.0
|
CA
|
A:ASN528
|
4.4
|
14.6
|
1.0
|
N
|
A:VAL529
|
4.5
|
16.0
|
1.0
|
CA
|
A:PRO534
|
4.5
|
26.7
|
1.0
|
CA
|
A:VAL529
|
4.5
|
16.1
|
1.0
|
CB
|
A:ASN528
|
4.5
|
14.7
|
1.0
|
N
|
A:GLU640
|
4.6
|
19.8
|
1.0
|
CA
|
A:PHE537
|
4.6
|
18.4
|
1.0
|
C
|
A:VAL529
|
4.7
|
16.0
|
1.0
|
C
|
A:PRO534
|
4.8
|
25.8
|
1.0
|
CA
|
A:GLU640
|
4.8
|
19.9
|
1.0
|
N
|
A:ASP531
|
4.8
|
18.8
|
1.0
|
N
|
A:SER535
|
4.9
|
25.5
|
1.0
|
N
|
A:GLY532
|
4.9
|
21.9
|
1.0
|
CG2
|
A:THR536
|
4.9
|
18.7
|
1.0
|
CB
|
A:ALA639
|
4.9
|
18.3
|
1.0
|
O
|
A:VAL529
|
4.9
|
16.3
|
1.0
|
C
|
A:GLY532
|
4.9
|
24.9
|
1.0
|
CB
|
A:GLU640
|
5.0
|
20.2
|
1.0
|
|
Sodium binding site 2 out
of 3 in 2bzd
Go back to
Sodium Binding Sites List in 2bzd
Sodium binding site 2 out
of 3 in the Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Na1648
b:8.4
occ:1.00
|
O
|
B:ALA639
|
2.3
|
16.2
|
1.0
|
O
|
B:ASN528
|
2.3
|
16.1
|
1.0
|
OE1
|
B:GLU640
|
2.3
|
21.6
|
0.5
|
O
|
B:ASN533
|
2.4
|
21.3
|
1.0
|
O
|
B:THR536
|
2.5
|
18.2
|
1.0
|
OD1
|
B:ASP531
|
2.7
|
12.1
|
1.0
|
OG1
|
B:THR536
|
2.7
|
17.8
|
1.0
|
C
|
B:THR536
|
3.3
|
19.1
|
1.0
|
C
|
B:ALA639
|
3.4
|
15.7
|
1.0
|
C
|
B:ASN528
|
3.5
|
15.7
|
1.0
|
C
|
B:ASN533
|
3.5
|
20.6
|
1.0
|
CD
|
B:GLU640
|
3.6
|
18.8
|
0.5
|
CG
|
B:ASP531
|
3.6
|
16.2
|
1.0
|
CB
|
B:THR536
|
3.9
|
18.5
|
1.0
|
OD2
|
B:ASP531
|
4.0
|
17.0
|
1.0
|
CA
|
B:THR536
|
4.0
|
18.8
|
1.0
|
N
|
B:THR536
|
4.0
|
18.3
|
1.0
|
N
|
B:ASN533
|
4.1
|
19.7
|
1.0
|
CA
|
B:ASN533
|
4.2
|
21.1
|
1.0
|
CA
|
B:ALA639
|
4.2
|
15.9
|
1.0
|
CG
|
B:GLU640
|
4.2
|
17.7
|
0.5
|
N
|
B:PHE537
|
4.3
|
18.2
|
1.0
|
CA
|
B:VAL529
|
4.3
|
14.7
|
1.0
|
CB
|
B:ASN533
|
4.3
|
20.1
|
1.0
|
CG
|
B:GLU640
|
4.3
|
15.2
|
0.5
|
CA
|
B:ASN528
|
4.3
|
15.4
|
1.0
|
N
|
B:VAL529
|
4.3
|
15.3
|
1.0
|
N
|
B:GLU640
|
4.4
|
16.2
|
1.0
|
CB
|
B:ASN528
|
4.5
|
15.8
|
1.0
|
N
|
B:PRO534
|
4.5
|
21.4
|
1.0
|
OE2
|
B:GLU640
|
4.5
|
15.8
|
0.5
|
C
|
B:VAL529
|
4.6
|
14.7
|
1.0
|
CA
|
B:PRO534
|
4.7
|
20.7
|
1.0
|
CA
|
B:PHE537
|
4.7
|
17.3
|
1.0
|
CA
|
B:GLU640
|
4.7
|
16.4
|
1.0
|
CB
|
B:ALA639
|
4.8
|
14.9
|
1.0
|
N
|
B:ASP531
|
4.9
|
16.0
|
1.0
|
C
|
B:PRO534
|
4.9
|
20.0
|
1.0
|
O
|
B:VAL529
|
4.9
|
15.3
|
1.0
|
CB
|
B:ASP531
|
4.9
|
16.9
|
1.0
|
N
|
B:GLY532
|
4.9
|
16.9
|
1.0
|
CG2
|
B:THR536
|
5.0
|
19.4
|
1.0
|
|
Sodium binding site 3 out
of 3 in 2bzd
Go back to
Sodium Binding Sites List in 2bzd
Sodium binding site 3 out
of 3 in the Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase.
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Na1648
b:16.9
occ:1.00
|
O
|
C:ASN533
|
2.3
|
29.6
|
1.0
|
O
|
C:THR536
|
2.4
|
20.6
|
1.0
|
O
|
C:ASN528
|
2.4
|
21.2
|
1.0
|
OG1
|
C:THR536
|
2.6
|
20.0
|
1.0
|
O
|
C:ALA639
|
2.6
|
17.7
|
1.0
|
OD1
|
C:ASP531
|
2.7
|
28.6
|
1.0
|
OE2
|
C:GLU640
|
2.9
|
33.1
|
1.0
|
C
|
C:THR536
|
3.3
|
20.8
|
1.0
|
C
|
C:ASN528
|
3.4
|
20.4
|
1.0
|
C
|
C:ASN533
|
3.5
|
29.4
|
1.0
|
CG
|
C:ASP531
|
3.6
|
26.8
|
1.0
|
C
|
C:ALA639
|
3.7
|
16.0
|
1.0
|
OD2
|
C:ASP531
|
3.8
|
19.1
|
1.0
|
CB
|
C:THR536
|
3.8
|
21.5
|
1.0
|
CA
|
C:THR536
|
3.9
|
21.1
|
1.0
|
CD
|
C:GLU640
|
4.0
|
27.6
|
1.0
|
N
|
C:THR536
|
4.0
|
21.6
|
1.0
|
N
|
C:PHE537
|
4.2
|
20.8
|
1.0
|
N
|
C:ASN533
|
4.2
|
28.8
|
1.0
|
CA
|
C:ASN533
|
4.2
|
29.6
|
1.0
|
CB
|
C:ASN533
|
4.2
|
29.6
|
1.0
|
CA
|
C:ALA639
|
4.3
|
15.8
|
1.0
|
CA
|
C:ASN528
|
4.3
|
19.1
|
1.0
|
N
|
C:VAL529
|
4.3
|
19.2
|
1.0
|
CA
|
C:VAL529
|
4.4
|
18.5
|
1.0
|
CB
|
C:ASN528
|
4.4
|
19.3
|
1.0
|
CA
|
C:PHE537
|
4.5
|
20.8
|
1.0
|
N
|
C:PRO534
|
4.5
|
28.9
|
1.0
|
CA
|
C:PRO534
|
4.6
|
27.7
|
1.0
|
CG
|
C:GLU640
|
4.7
|
22.4
|
1.0
|
C
|
C:PRO534
|
4.7
|
26.1
|
1.0
|
CB
|
C:ALA639
|
4.8
|
15.9
|
1.0
|
OE1
|
C:GLU640
|
4.8
|
27.6
|
1.0
|
N
|
C:GLU640
|
4.8
|
15.2
|
1.0
|
C
|
C:VAL529
|
4.9
|
19.1
|
1.0
|
N
|
C:SER535
|
4.9
|
24.1
|
1.0
|
CB
|
C:ASP531
|
4.9
|
26.6
|
1.0
|
N
|
C:ASP531
|
5.0
|
25.5
|
1.0
|
CG
|
C:ASN533
|
5.0
|
30.8
|
1.0
|
|
Reference:
S.L.Newstead,
J.N.Watson,
A.J.Bennet,
G.Taylor.
Galactose Recognition By the Carbohydrate-Binding Module of A Bacterial Sialidase. Acta Crystallogr.,Sect.D V. 61 1483 2005.
ISSN: ISSN 0907-4449
PubMed: 16239725
DOI: 10.1107/S0907444905026132
Page generated: Mon Oct 7 02:03:16 2024
|