Sodium in PDB 8afu: Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus

All present enzymatic activity of Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus:
1.2.1.38;

The structure of Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus, PDB code: 8afu was solved by P.Pfister, M.Nattermann, J.Zarzycki, T.J.Erb, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.29 / 1.99
Space group	I 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	86.698, 77.841, 122.026, 90, 92.87, 90
R / Rfree (%)	18.4 / 21.6

The binding sites of Sodium atom in the Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus (pdb code 8afu). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus, PDB code: 8afu:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus within 5.0Å range: probe atom residue distance (Å) B Occ A:Na401 b:37.4 occ:1.00 O A:HOH593 2.5 77.5 1.0 O A:PHE26 2.6 48.3 1.0 O A:HIS23 2.6 45.0 1.0 O A:LEU20 2.6 39.7 1.0 O A:ALA21 3.1 48.8 1.0 O A:HOH592 3.5 46.9 1.0 C A:ALA21 3.6 42.0 1.0 N A:HIS23 3.7 43.5 1.0 C A:HIS23 3.7 44.0 1.0 C A:PHE26 3.8 47.1 1.0 C A:LEU20 3.8 38.9 1.0 CA A:HIS23 4.1 44.3 1.0 CA A:ALA21 4.1 41.9 1.0 C A:ASN22 4.3 44.5 1.0 CB A:HIS23 4.3 42.8 1.0 N A:ASN22 4.3 41.2 1.0 N A:ALA21 4.5 39.4 1.0 CA A:GLU27 4.5 46.3 1.0 N A:GLU27 4.6 45.6 1.0 CA A:ASN22 4.6 47.9 1.0 CA A:PHE26 4.8 45.9 1.0 N A:PHE26 4.8 45.3 1.0 N A:PRO24 4.9 45.6 1.0 CA A:LEU20 5.0 38.9 1.0

Sodium binding site 2 out of 2 in the Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Daargc - N-Acetyl-Gamma-Glutamyl-Phosphate Reductase of Denitrovibrio Acetiphilus within 5.0Å range: probe atom residue distance (Å) B Occ B:Na401 b:33.8 occ:1.00 O B:PHE26 2.5 40.9 1.0 O B:LEU20 2.6 36.6 1.0 O B:HIS23 2.6 44.2 1.0 O B:ALA21 3.1 46.5 1.0 O B:HOH544 3.1 55.2 1.0 N B:HIS23 3.6 43.1 1.0 C B:ALA21 3.6 41.7 1.0 C B:HIS23 3.6 46.1 1.0 C B:LEU20 3.7 39.8 1.0 C B:PHE26 3.8 41.9 1.0 O B:HOH577 3.9 41.8 1.0 CA B:HIS23 4.0 40.2 1.0 CA B:ALA21 4.0 40.1 1.0 CB B:HIS23 4.1 39.1 1.0 C B:ASN22 4.2 44.5 1.0 N B:ASN22 4.3 42.0 1.0 N B:ALA21 4.3 36.5 1.0 O B:HOH508 4.5 60.8 1.0 CA B:GLU27 4.5 43.0 1.0 N B:GLU27 4.6 40.2 1.0 CA B:ASN22 4.6 44.5 1.0 N B:PHE26 4.8 43.0 1.0 CA B:PHE26 4.8 43.4 1.0 N B:PRO24 4.8 42.7 1.0 O B:HOH580 4.8 51.1 1.0 O B:ASN22 4.9 45.1 1.0 CA B:LEU20 4.9 37.5 1.0

M.Nattermann, S.Wenk, P.Pfister, N.Guntermann, L.Nickel, J.Zarzycki, G.Francio, W.Leitner, A.Bar-Even, T.J.Erb. Engineering A Two-Enzyme Cascade For in Vivo Formate Assimilation. To Be Published.