Sodium in PDB 7m45: Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
Enzymatic activity of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
All present enzymatic activity of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec:
2.7.7.7;
Protein crystallography data
The structure of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec, PDB code: 7m45
was solved by
J.A.Jamsen,
S.H.Wilson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
24.02 /
1.89
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
56.018,
62.547,
140.01,
90,
90,
90
|
R / Rfree (%)
|
20.5 /
24.2
|
Other elements in 7m45:
The structure of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec also contains other interesting chemical elements:
Sodium Binding Sites:
The binding sites of Sodium atom in the Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
(pdb code 7m45). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 4 binding sites of Sodium where determined in the
Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec, PDB code: 7m45:
Jump to Sodium binding site number:
1;
2;
3;
4;
Sodium binding site 1 out
of 4 in 7m45
Go back to
Sodium Binding Sites List in 7m45
Sodium binding site 1 out
of 4 in the Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na604
b:21.0
occ:1.00
|
O
|
A:ALA344
|
2.3
|
20.1
|
1.0
|
O
|
A:SER339
|
2.3
|
19.9
|
0.4
|
OP1
|
P:DA5
|
2.4
|
21.8
|
1.0
|
O
|
A:ILE341
|
2.4
|
22.3
|
1.0
|
O
|
A:SER339
|
2.5
|
19.5
|
0.6
|
O
|
P:HOH128
|
2.5
|
28.3
|
1.0
|
O
|
P:HOH111
|
2.6
|
26.1
|
1.0
|
C
|
A:ILE341
|
3.3
|
21.7
|
1.0
|
C
|
A:ALA344
|
3.4
|
20.5
|
1.0
|
C
|
A:SER339
|
3.4
|
21.9
|
0.4
|
P
|
P:DA5
|
3.4
|
31.1
|
1.0
|
C
|
A:SER339
|
3.4
|
22.4
|
0.6
|
OP2
|
P:DA5
|
3.6
|
18.3
|
1.0
|
O
|
A:HOH800
|
3.8
|
38.4
|
1.0
|
N
|
A:ALA344
|
3.8
|
17.9
|
1.0
|
CA
|
A:TRP342
|
3.9
|
19.7
|
1.0
|
N
|
A:GLY343
|
4.0
|
24.2
|
1.0
|
N
|
A:TRP342
|
4.0
|
20.8
|
1.0
|
N
|
A:ILE341
|
4.1
|
17.7
|
1.0
|
CA
|
A:ALA344
|
4.1
|
23.8
|
1.0
|
CA
|
A:SER339
|
4.1
|
20.0
|
0.6
|
CA
|
A:SER339
|
4.2
|
21.0
|
0.4
|
C
|
A:ASN340
|
4.3
|
19.8
|
1.0
|
C
|
A:TRP342
|
4.3
|
22.4
|
1.0
|
N
|
A:ASN340
|
4.3
|
19.0
|
1.0
|
O3'
|
P:DT4
|
4.4
|
24.6
|
1.0
|
CA
|
A:ILE341
|
4.4
|
17.5
|
1.0
|
CA
|
A:ASN340
|
4.4
|
20.2
|
1.0
|
N
|
A:GLY345
|
4.4
|
18.4
|
1.0
|
O
|
P:HOH126
|
4.5
|
24.3
|
0.5
|
CB
|
A:ALA344
|
4.6
|
23.1
|
1.0
|
O
|
P:HOH126
|
4.6
|
22.2
|
0.5
|
O5'
|
P:DA5
|
4.6
|
18.1
|
1.0
|
CB
|
A:SER339
|
4.7
|
23.4
|
0.6
|
CA
|
A:GLY345
|
4.7
|
20.0
|
1.0
|
C
|
A:GLY343
|
4.7
|
22.3
|
1.0
|
O
|
A:ASN340
|
4.8
|
23.1
|
1.0
|
CA
|
A:GLY343
|
4.9
|
22.2
|
1.0
|
CB
|
A:SER339
|
4.9
|
22.9
|
0.4
|
O
|
A:HOH830
|
5.0
|
45.4
|
1.0
|
|
Sodium binding site 2 out
of 4 in 7m45
Go back to
Sodium Binding Sites List in 7m45
Sodium binding site 2 out
of 4 in the Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na605
b:53.3
occ:1.00
|
O
|
D:HOH102
|
2.2
|
50.2
|
1.0
|
O
|
A:ILE302
|
2.3
|
64.0
|
1.0
|
O
|
A:CYS300
|
2.6
|
82.8
|
1.0
|
O
|
A:ILE305
|
2.9
|
59.8
|
1.0
|
C
|
A:ILE302
|
3.1
|
66.3
|
1.0
|
OP1
|
D:DC3
|
3.1
|
55.4
|
1.0
|
C
|
A:CYS300
|
3.6
|
84.5
|
1.0
|
N
|
A:PRO303
|
3.6
|
70.8
|
1.0
|
CA
|
A:PRO303
|
3.6
|
66.2
|
1.0
|
N
|
A:ILE305
|
3.9
|
50.3
|
1.0
|
C
|
A:ILE305
|
3.9
|
55.6
|
1.0
|
N
|
A:ILE302
|
3.9
|
67.9
|
1.0
|
OP2
|
D:DC3
|
4.0
|
55.1
|
1.0
|
C
|
A:SER301
|
4.0
|
68.8
|
1.0
|
P
|
D:DC3
|
4.0
|
53.6
|
1.0
|
N
|
A:GLY304
|
4.1
|
49.6
|
1.0
|
O
|
A:SER301
|
4.1
|
68.2
|
1.0
|
CA
|
A:ILE302
|
4.2
|
66.3
|
1.0
|
C
|
A:PRO303
|
4.2
|
63.0
|
1.0
|
CA
|
A:CYS300
|
4.3
|
85.2
|
1.0
|
CA
|
A:ILE305
|
4.4
|
53.1
|
1.0
|
N
|
A:SER301
|
4.5
|
78.0
|
1.0
|
CB
|
A:ILE305
|
4.6
|
56.7
|
1.0
|
CA
|
A:SER301
|
4.7
|
75.4
|
1.0
|
O
|
A:ALA299
|
4.8
|
68.6
|
1.0
|
CD
|
A:PRO303
|
4.8
|
72.1
|
1.0
|
C
|
A:GLY304
|
4.9
|
53.4
|
1.0
|
CB
|
A:PRO303
|
5.0
|
65.9
|
1.0
|
|
Sodium binding site 3 out
of 4 in 7m45
Go back to
Sodium Binding Sites List in 7m45
Sodium binding site 3 out
of 4 in the Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na606
b:30.5
occ:1.00
|
OD1
|
A:ASP429
|
2.1
|
28.4
|
1.0
|
OD2
|
A:ASP427
|
2.2
|
35.1
|
0.4
|
OD2
|
A:ASP490
|
2.3
|
24.5
|
1.0
|
OP1
|
P:DT7
|
2.4
|
22.7
|
0.6
|
O
|
A:HOH818
|
2.4
|
26.2
|
0.6
|
O
|
A:HOH775
|
2.5
|
28.1
|
0.6
|
O3'
|
P:DC6
|
2.6
|
30.7
|
0.6
|
O3'
|
P:DC6
|
2.6
|
29.2
|
0.4
|
O1A
|
A:TTP602
|
2.7
|
23.3
|
0.4
|
CG
|
A:ASP427
|
3.0
|
34.2
|
0.4
|
P
|
P:DT7
|
3.1
|
26.5
|
0.6
|
OD1
|
A:ASP427
|
3.1
|
32.8
|
0.6
|
CG
|
A:ASP490
|
3.1
|
26.2
|
1.0
|
OD1
|
A:ASP427
|
3.2
|
33.7
|
0.4
|
CG
|
A:ASP429
|
3.2
|
32.9
|
1.0
|
CG
|
A:ASP427
|
3.5
|
34.4
|
0.6
|
MG
|
A:MG603
|
3.5
|
25.8
|
1.0
|
OD2
|
A:ASP429
|
3.6
|
30.2
|
1.0
|
C3'
|
P:DC6
|
3.7
|
27.4
|
0.4
|
PA
|
A:TTP602
|
3.7
|
27.4
|
0.4
|
CB
|
A:ASP490
|
3.8
|
21.0
|
1.0
|
C3'
|
P:DC6
|
3.8
|
26.8
|
0.6
|
OD2
|
A:ASP427
|
3.9
|
34.4
|
0.6
|
O5'
|
A:TTP602
|
3.9
|
26.2
|
0.4
|
OD1
|
A:ASP490
|
4.0
|
26.9
|
1.0
|
C4'
|
P:DC6
|
4.1
|
26.1
|
0.6
|
O2A
|
A:TTP602
|
4.1
|
27.2
|
0.4
|
OP2
|
P:DT7
|
4.2
|
27.2
|
0.6
|
O5'
|
P:DT7
|
4.2
|
25.0
|
0.6
|
CB
|
A:ASP427
|
4.3
|
31.0
|
0.6
|
C5'
|
A:TTP602
|
4.3
|
26.6
|
0.4
|
C5'
|
P:DC6
|
4.3
|
23.9
|
0.6
|
C5'
|
P:DC6
|
4.3
|
24.3
|
0.4
|
CB
|
A:ASP427
|
4.3
|
30.7
|
0.4
|
C4'
|
P:DC6
|
4.3
|
26.4
|
0.4
|
C5'
|
P:DT7
|
4.4
|
27.1
|
0.6
|
CB
|
A:ASP429
|
4.5
|
28.6
|
1.0
|
O2G
|
A:TTP602
|
4.5
|
39.8
|
0.4
|
NH2
|
A:ARG488
|
4.6
|
26.9
|
1.0
|
O
|
A:VAL428
|
4.7
|
23.3
|
1.0
|
O5'
|
P:DC6
|
4.8
|
22.4
|
0.4
|
O5'
|
P:DC6
|
4.8
|
21.2
|
0.6
|
C2'
|
P:DC6
|
4.9
|
26.6
|
0.6
|
CA
|
A:ASP429
|
4.9
|
23.5
|
1.0
|
CE1
|
A:PHE506
|
5.0
|
28.5
|
1.0
|
OP1
|
P:DC6
|
5.0
|
21.2
|
0.4
|
O1B
|
A:TTP602
|
5.0
|
32.3
|
0.4
|
N
|
A:ASP490
|
5.0
|
21.4
|
1.0
|
|
Sodium binding site 4 out
of 4 in 7m45
Go back to
Sodium Binding Sites List in 7m45
Sodium binding site 4 out
of 4 in the Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 4 of Dna Polymerase Lambda, Ttp:at MG2+ Reaction State Ternary Complex, 120 Sec within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na607
b:72.7
occ:0.97
|
NE2
|
A:GLN366
|
2.4
|
58.5
|
1.0
|
O
|
A:HOH848
|
3.0
|
75.0
|
1.0
|
O
|
A:HOH811
|
3.0
|
61.8
|
1.0
|
O
|
A:HOH829
|
3.2
|
50.0
|
1.0
|
O
|
A:HOH705
|
3.4
|
68.3
|
1.0
|
CD
|
A:GLN366
|
3.5
|
55.6
|
1.0
|
OE1
|
A:GLN366
|
3.8
|
55.7
|
1.0
|
CG
|
A:GLN366
|
4.8
|
49.1
|
1.0
|
CE1
|
A:PHE357
|
4.9
|
42.0
|
1.0
|
|
Reference:
J.A.Jamsen,
D.D.Shock,
S.H.Wilson.
Watching Right and Wrong Nucleotide Insertion Captures Hidden Polymerase Fidelity Checkpoints. Nat Commun V. 13 3193 2022.
ISSN: ESSN 2041-1723
PubMed: 35680862
DOI: 10.1038/S41467-022-30141-W
Page generated: Tue Oct 8 17:49:49 2024
|