Sodium in PDB 7awm: Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101

The structure of Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101, PDB code: 7awm was solved by J.C.Canul-Tec, P.Legrand, N.Reyes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.31 / 3.25
Space group	P 63
Cell size a, b, c (Å), α, β, γ (°)	122.882, 122.882, 90.032, 90, 90, 120
R / Rfree (%)	21.9 / 23.8

The structure of Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101 also contains other interesting chemical elements:

Barium

(Ba)

1 atom

The binding sites of Sodium atom in the Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101 (pdb code 7awm). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101, PDB code: 7awm:
Jump to Sodium binding site number: 1; 2; 3;

Sodium binding site 1 out of 3 in the Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101 within 5.0Å range: probe atom residue distance (Å) B Occ A:Na601 b:125.5 occ:1.00 O A:GLY374 2.7 108.3 1.0 CA A:ALA375 3.1 103.4 1.0 O A:ALA375 3.1 108.1 1.0 OD2 A:ASP467 3.2 126.0 1.0 O A:ASN378 3.2 120.8 1.0 OG A:SER345 3.2 113.1 1.0 C A:ALA375 3.2 108.2 1.0 O A:ASN463 3.3 107.3 1.0 CB A:SER345 3.5 102.6 1.0 C A:GLY374 3.5 107.8 1.0 N A:ALA375 3.7 103.8 1.0 OD1 A:ASP467 3.8 124.3 1.0 C A:ASN463 3.8 108.5 1.0 CB A:ASN463 3.9 102.6 1.0 CG A:ASP467 3.9 121.0 1.0 CE A:MET379 4.0 118.9 1.0 C A:ASN378 4.1 122.2 1.0 N A:THR376 4.2 105.5 1.0 CB A:ALA375 4.3 102.2 1.0 CA A:ASN463 4.4 105.3 1.0 CG A:MET379 4.5 122.7 1.0 N A:VAL464 4.6 105.3 1.0 SD A:MET379 4.6 125.1 1.0 CG A:ASN463 4.8 125.8 1.0 N A:MET379 4.9 120.2 1.0 CA A:GLY374 4.9 103.8 1.0 CA A:SER345 4.9 98.5 1.0 N A:ASN378 4.9 115.2 1.0 CA A:ASN378 4.9 117.8 1.0 CA A:MET379 5.0 120.6 1.0 CA A:VAL464 5.0 104.0 1.0

Sodium binding site 2 out of 3 in the Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101 within 5.0Å range: probe atom residue distance (Å) B Occ A:Na602 b:139.9 occ:1.00 OD1 A:ASN378 2.2 143.6 1.0 O A:TYR127 2.3 132.1 1.0 OG1 A:THR130 2.4 132.9 1.0 OG1 A:THR131 2.5 131.2 1.0 OD1 A:ASP380 2.6 144.8 1.0 C A:TYR127 3.1 131.7 1.0 N A:THR131 3.2 131.3 1.0 CA A:TYR127 3.3 124.8 1.0 CG A:ASP380 3.4 142.8 1.0 CG A:ASN378 3.4 147.5 1.0 CB A:THR131 3.5 135.0 1.0 CB A:THR130 3.5 136.7 1.0 OD2 A:ASP380 3.7 147.9 1.0 C A:THR130 3.8 136.4 1.0 CA A:THR131 3.8 130.6 1.0 CA A:THR130 4.0 133.0 1.0 O A:TYR126 4.2 134.2 1.0 CB A:TYR127 4.2 123.0 1.0 ND2 A:ASN378 4.3 139.4 1.0 N A:THR130 4.4 134.1 1.0 N A:MET128 4.4 130.3 1.0 CB A:ASN378 4.4 119.9 1.0 N A:TYR127 4.5 126.8 1.0 CA A:ASN378 4.5 117.8 1.0 O A:THR130 4.7 137.5 1.0 CD1 A:TYR127 4.7 122.9 1.0 CB A:ASP380 4.7 132.6 1.0 C A:TYR126 4.7 133.0 1.0 CG2 A:THR130 4.8 136.3 1.0 CG2 A:THR131 4.8 133.3 1.0 N A:ASP380 4.9 126.3 1.0 C A:ASN378 4.9 122.2 1.0 CG A:TYR127 5.0 121.8 1.0 N A:MET379 5.0 120.2 1.0 CA A:ASP380 5.0 129.5 1.0

Sodium binding site 3 out of 3 in the Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Structure of the Thermostabilized EAAT1 Cryst Mutant in Complex with L-Asp, Three Sodium Ions and the Allosteric Inhibitor UCPH101 within 5.0Å range: probe atom residue distance (Å) B Occ A:Na603 b:90.1 occ:1.00 O A:ALA420 2.2 115.0 1.0 O A:THR376 2.2 111.3 1.0 O A:SER417 2.3 117.2 1.0 CG A:MET379 2.8 122.7 1.0 O A:ILE418 3.1 115.0 1.0 CE A:MET379 3.2 118.9 1.0 SD A:MET379 3.2 125.1 1.0 C A:THR376 3.4 111.4 1.0 C A:ALA420 3.4 115.1 1.0 C A:SER417 3.4 119.8 1.0 C A:ILE418 3.5 117.6 1.0 N A:ALA420 3.8 114.8 1.0 CA A:ILE418 3.9 114.6 1.0 CB A:MET379 3.9 121.8 1.0 CA A:ILE377 3.9 110.6 1.0 CA A:ALA420 4.0 113.4 1.0 N A:ILE418 4.1 116.9 1.0 N A:ILE377 4.1 108.7 1.0 C A:GLY419 4.1 116.8 1.0 O A:ILE377 4.1 120.4 1.0 N A:GLY419 4.2 115.8 1.0 C A:ILE377 4.2 118.2 1.0 CB A:ALA420 4.3 116.1 1.0 O A:GLY419 4.3 114.2 1.0 CA A:THR376 4.4 105.6 1.0 N A:ALA421 4.5 109.2 1.0 CA A:SER417 4.6 118.4 1.0 CA A:GLY419 4.7 114.4 1.0 O A:ALA416 4.8 119.1 1.0 CA A:ALA421 4.9 106.5 1.0 OG1 A:THR376 4.9 116.8 1.0 N A:MET379 5.0 120.2 1.0

J.C.Canul-Tec, J.Dhenin, R.Assal, P.Legrand, R.Martial, J.Chamot-Rooke, N.Reyes. Ion-Coupling Mechanism of Excitatory Amino Acid Transporters To Be Published.