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Sodium in PDB 5vs2: Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation

Enzymatic activity of Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation

All present enzymatic activity of Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation:
2.7.7.7;

Protein crystallography data

The structure of Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation, PDB code: 5vs2 was solved by A.J.Reed, Z.Suo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.64 / 2.33
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 50.940, 80.490, 55.660, 90.00, 107.28, 90.00
R / Rfree (%) 18.7 / 25

Other elements in 5vs2:

The structure of Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation also contains other interesting chemical elements:

Calcium (Ca) 3 atoms

Sodium Binding Sites:

The binding sites of Sodium atom in the Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation (pdb code 5vs2). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation, PDB code: 5vs2:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 5vs2

Go back to Sodium Binding Sites List in 5vs2
Sodium binding site 1 out of 2 in the Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Na101

b:32.9
occ:1.00
O A:VAL65 2.5 27.1 1.0
O A:LYS60 2.5 32.1 1.0
O A:LEU62 2.6 30.0 1.0
OP1 D:DC3 2.6 30.7 1.0
OP2 D:DC3 3.5 26.4 1.0
P D:DC3 3.5 34.5 1.0
C A:LEU62 3.6 28.3 1.0
C A:LYS60 3.6 33.8 1.0
C A:VAL65 3.6 28.2 1.0
N A:VAL65 4.0 25.8 1.0
C A:LYS61 4.1 31.2 1.0
N A:LEU62 4.2 29.6 1.0
CA A:PRO63 4.2 29.1 1.0
O A:LYS61 4.3 29.1 1.0
N A:GLY64 4.3 27.2 1.0
N A:PRO63 4.3 28.4 1.0
CA A:VAL65 4.3 26.5 1.0
CA A:LYS60 4.5 32.1 1.0
CA A:LEU62 4.5 28.5 1.0
N A:LYS61 4.5 30.8 1.0
CB A:VAL65 4.6 28.9 1.0
CA A:LYS61 4.6 32.2 1.0
O3' D:DT2 4.6 29.0 1.0
C A:PRO63 4.7 27.6 1.0
N A:GLY66 4.7 30.4 1.0
O5' D:DC3 4.7 32.2 1.0
CG A:LYS60 4.9 39.0 1.0
CA A:GLY66 4.9 29.8 1.0
C A:GLY64 4.9 25.5 1.0

Sodium binding site 2 out of 2 in 5vs2

Go back to Sodium Binding Sites List in 5vs2
Sodium binding site 2 out of 2 in the Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Human Dna Polymerase Beta Pre-Catalytic 8-Oxog:Da Extension Complex with Dttp Bound in Watson-Crick Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na403

b:14.1
occ:1.00
O A:HOH565 2.1 22.1 1.0
OE2 A:GLU249 2.3 28.3 1.0
O A:HOH584 2.4 23.1 1.0
OE1 A:GLU249 2.5 27.8 1.0
CD A:GLU249 2.8 28.6 1.0
O A:HOH502 4.1 24.4 1.0
CG A:GLU249 4.3 26.4 1.0
OE1 A:GLN240 4.6 19.1 1.0
O A:HOH549 4.8 17.9 1.0
NE2 A:GLN240 4.9 18.8 1.0

Reference:

A.J.Reed, Z.Suo. Time-Dependent Extension From An 8-Oxoguanine Lesion By Human Dna Polymerase Beta. J. Am. Chem. Soc. V. 139 9684 2017.
ISSN: ESSN 1520-5126
PubMed: 28682600
DOI: 10.1021/JACS.7B05048
Page generated: Tue Oct 8 00:55:31 2024

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