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Sodium in PDB 4z6c: Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp

Enzymatic activity of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp

All present enzymatic activity of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp:
2.7.7.7;

Protein crystallography data

The structure of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp, PDB code: 4z6c was solved by M.-C.Koag, S.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.68
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 48.180, 79.274, 54.372, 90.00, 105.96, 90.00
R / Rfree (%) 20.1 / 27.2

Other elements in 4z6c:

The structure of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Sodium Binding Sites:

The binding sites of Sodium atom in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp (pdb code 4z6c). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp, PDB code: 4z6c:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 4z6c

Go back to Sodium Binding Sites List in 4z6c
Sodium binding site 1 out of 2 in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Na102

b:25.9
occ:1.00
O A:THR101 2.3 24.4 1.0
OP1 P:DG9 2.3 27.5 1.0
O A:HOH522 2.4 29.4 1.0
O A:ILE106 2.6 26.8 1.0
OP2 P:DG9 2.8 24.3 1.0
O A:VAL103 2.8 23.1 1.0
P P:DG9 2.9 22.1 1.0
C A:THR101 3.5 23.9 1.0
C A:ILE106 3.6 25.0 1.0
C A:VAL103 3.7 24.7 1.0
O5' P:DG9 4.0 28.2 1.0
N A:ILE106 4.2 24.1 1.0
O3' P:DC8 4.2 31.4 1.0
CA A:SER104 4.2 19.8 1.0
N A:GLY105 4.2 22.4 1.0
CA A:THR101 4.3 24.8 1.0
N A:SER104 4.3 21.9 1.0
C A:ARG102 4.4 20.7 1.0
N A:VAL103 4.4 21.0 1.0
N A:ARG102 4.4 19.5 1.0
CA A:ILE106 4.4 22.9 1.0
N A:GLY107 4.5 25.4 1.0
CA A:ARG102 4.6 19.6 1.0
CA A:GLY107 4.6 17.6 1.0
C A:SER104 4.7 23.2 1.0
CA A:VAL103 4.7 20.9 1.0
O A:ARG102 4.7 18.6 1.0
CB A:THR101 4.8 25.1 1.0
C5' P:DG9 4.9 29.1 1.0
C A:GLY105 5.0 24.5 1.0

Sodium binding site 2 out of 2 in 4z6c

Go back to Sodium Binding Sites List in 4z6c
Sodium binding site 2 out of 2 in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Na101

b:30.3
occ:1.00
OP1 D:DC3 2.4 31.7 1.0
O A:LYS60 2.6 35.2 1.0
O A:VAL65 2.7 32.1 1.0
O D:HOH201 2.9 25.1 1.0
O A:LEU62 3.0 30.6 1.0
P D:DC3 3.3 34.8 1.0
OP2 D:DC3 3.4 38.3 1.0
C A:VAL65 3.8 27.4 1.0
C A:LYS60 3.8 30.4 1.0
C A:LEU62 3.9 32.2 1.0
CA A:PRO63 4.2 34.1 1.0
N A:VAL65 4.3 32.4 1.0
N A:GLY64 4.4 32.3 1.0
O5' D:DC3 4.4 31.4 1.0
C A:LYS61 4.4 32.8 1.0
N A:PRO63 4.5 30.3 1.0
O3' D:DT2 4.5 36.0 1.0
CA A:VAL65 4.5 26.9 1.0
O A:LYS61 4.6 33.8 1.0
N A:LEU62 4.6 32.4 1.0
CA A:LYS60 4.6 28.2 1.0
N A:LYS61 4.7 33.7 1.0
N A:GLY66 4.7 24.7 1.0
CA A:LYS61 4.7 33.4 1.0
C A:PRO63 4.7 34.1 1.0
CG A:LYS60 4.7 26.2 1.0
CA A:GLY66 4.8 28.4 1.0
NZ A:LYS60 4.9 31.9 1.0
CA A:LEU62 4.9 30.5 1.0
CB A:VAL65 5.0 29.7 1.0

Reference:

M.C.Koag, S.Lee. Insights Into the Effect of Minor Groove Interactions and Metal Cofactors on Mutagenic Replication By Human Dna Polymerase Beta. Biochem. J. V. 475 571 2018.
ISSN: ESSN 1470-8728
PubMed: 29301983
DOI: 10.1042/BCJ20170787
Page generated: Tue Dec 15 10:05:39 2020

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