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Sodium in PDB 3wdr: Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide

Enzymatic activity of Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide

All present enzymatic activity of Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide:
3.2.1.78;

Protein crystallography data

The structure of Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide, PDB code: 3wdr was solved by H.Tsukagoshi, T.Ishida, K.K.Touhara, K.Igarashi, M.Samejima, S.Fushinobu, K.Kitamoto, M.Arioka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 36.51 / 1.40
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 45.522, 61.644, 58.068, 90.00, 92.27, 90.00
R / Rfree (%) 13.7 / 16

Other elements in 3wdr:

The structure of Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide (pdb code 3wdr). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide, PDB code: 3wdr:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 3wdr

Go back to Sodium Binding Sites List in 3wdr
Sodium binding site 1 out of 2 in the Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na409

b:9.6
occ:1.00
OH A:TYR270 2.9 12.5 1.0
O A:HOH830 2.9 26.6 1.0
O A:HOH550 3.0 14.6 1.0
N A:ILE237 3.3 7.9 1.0
CA A:PRO236 3.6 7.4 1.0
CE1 A:TYR270 3.7 9.8 1.0
CZ A:TYR270 3.7 9.3 1.0
CG2 A:THR235 3.7 6.9 1.0
CB A:THR235 3.8 7.3 1.0
CG2 A:ILE237 3.9 8.4 1.0
C A:PRO236 4.0 7.8 1.0
CB A:PRO236 4.2 7.7 1.0
CA A:THR235 4.3 7.0 1.0
CB A:ILE237 4.3 8.4 1.0
CA A:ILE237 4.4 8.2 1.0
N A:PRO236 4.6 7.4 1.0
O A:HOH695 4.9 29.4 1.0
N A:GLU238 4.9 10.5 1.0
CD1 A:TYR270 4.9 8.9 1.0
O A:HOH725 5.0 27.7 1.0
OG1 A:THR235 5.0 7.4 1.0
CE2 A:TYR270 5.0 9.0 1.0
C A:THR235 5.0 7.4 1.0

Sodium binding site 2 out of 2 in 3wdr

Go back to Sodium Binding Sites List in 3wdr
Sodium binding site 2 out of 2 in the Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus Complexed with Gluco-Manno- Oligosaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na410

b:6.3
occ:1.00
O A:HOH632 3.2 12.9 1.0
O A:HOH780 3.3 27.7 1.0
O A:HOH634 3.3 15.1 1.0
N A:LEU323 3.3 7.4 1.0
CB A:SER322 3.8 7.0 1.0
CA A:SER322 3.8 6.9 1.0
CB A:PRO314 3.8 7.8 1.0
CG A:PRO319 4.0 9.6 1.0
CB A:LEU323 4.0 8.5 1.0
CG A:PRO314 4.0 7.4 1.0
C A:SER322 4.1 7.0 1.0
CA A:LEU323 4.3 8.0 1.0
CB A:PRO319 4.8 9.4 1.0
CD A:PRO319 4.9 9.6 1.0
OG A:SER322 4.9 8.3 1.0
O A:HOH633 4.9 24.5 1.0

Reference:

H.Tsukagoshi, A.Nakamura, T.Ishida, K.K.Touhara, M.Otagiri, S.Moriya, M.Samejima, K.Igarashi, S.Fushinobu, K.Kitamoto, M.Arioka. Structural and Biochemical Analyses of Glycoside Hydrolase Family 26 Beta-Mannanase From A Symbiotic Protist of the Termite Reticulitermes Speratus J.Biol.Chem. V. 289 10843 2014.
ISSN: ISSN 0021-9258
PubMed: 24570006
DOI: 10.1074/JBC.M114.555383
Page generated: Mon Oct 7 13:56:40 2024

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