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Sodium in PDB 3po4: Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp

Enzymatic activity of Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp

All present enzymatic activity of Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp:
2.7.7.7;

Protein crystallography data

The structure of Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp, PDB code: 3po4 was solved by A.Marx, K.Diederichs, S.Obeid, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.20 / 1.80
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 113.525, 113.525, 91.238, 90.00, 90.00, 120.00
R / Rfree (%) 16.4 / 19.4

Other elements in 3po4:

The structure of Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms

Sodium Binding Sites:

The binding sites of Sodium atom in the Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp (pdb code 3po4). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total only one binding site of Sodium was determined in the Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp, PDB code: 3po4:

Sodium binding site 1 out of 1 in 3po4

Go back to Sodium Binding Sites List in 3po4
Sodium binding site 1 out of 1 in the Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Structure of A Mutant of the Large Fragment of Dna Polymerase I From Thermus Aquaticus in Complex with A Blunt-Ended Dna and Ddatp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na833

b:50.7
occ:1.00
OE2 A:GLU507 2.5 35.4 1.0
CD A:GLU507 3.3 36.9 1.0
OE1 A:GLU507 3.4 38.5 1.0
CG A:GLU507 4.8 34.3 1.0

Reference:

S.Obeid, A.Schnur, C.Gloeckner, N.Blatter, W.Welte, K.Diederichs, A.Marx. Learning From Directed Evolution: Thermus Aquaticus Dna Polymerase Mutants with Translesion Synthesis Activity. Chembiochem V. 12 1574 2011.
ISSN: ISSN 1439-4227
PubMed: 21480455
DOI: 10.1002/CBIC.201000783
Page generated: Tue Dec 15 06:21:53 2020

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