Sodium in PDB 3kw8: Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution

All present enzymatic activity of Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution:
1.10.3.2;

The structure of Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution, PDB code: 3kw8 was solved by T.Skalova, J.Dohnalek, P.Kolenko, J.Duskova, A.Stepankova, J.Hasek, L.H.Ostergaard, P.R.Ostergaard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.56 / 2.29
Space group	P 43 3 2
Cell size a, b, c (Å), α, β, γ (°)	176.723, 176.723, 176.723, 90.00, 90.00, 90.00
R / Rfree (%)	16.1 / 17.8

The structure of Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution also contains other interesting chemical elements:

Iron	(Fe)	1 atom
Copper	(Cu)	4 atoms

The binding sites of Sodium atom in the Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution (pdb code 3kw8). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution, PDB code: 3kw8:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Na512 b:49.8 occ:1.00 O A:GLU80 2.8 29.7 1.0 O1 A:PGE612 3.0 53.3 1.0 O3 A:PGE612 3.0 49.8 1.0 O A:HOH783 3.0 52.0 1.0 O4 A:PGE612 3.0 58.7 1.0 O2 A:PGE612 3.2 45.5 1.0 C1 A:PGE612 3.7 49.0 1.0 C A:GLU80 3.8 28.9 1.0 C3 A:PGE612 3.8 44.6 1.0 C5 A:PGE612 3.9 52.6 1.0 C2 A:PGE612 3.9 46.9 1.0 C6 A:PGE612 3.9 55.5 1.0 C4 A:PGE612 3.9 46.0 1.0 CB A:GLU80 4.1 29.3 1.0 CA A:GLU80 4.3 29.3 1.0 N A:GLU80 4.5 28.8 1.0 CG A:GLU80 4.5 31.9 1.0 N A:VAL81 4.9 28.0 1.0

Sodium binding site 2 out of 2 in the Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Two-Domain Laccase From Streptomyces Coelicolor at 2.3 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Na513 b:68.8 occ:1.00 O2 A:PGE613 3.2 59.7 1.0 O1 A:PGE613 3.3 64.1 1.0 O3 A:PGE613 3.3 63.8 1.0 O A:HOH888 3.4 68.2 1.0 O4 A:PGE613 3.7 69.6 1.0 C2 A:PGE613 3.7 59.8 1.0 C4 A:PGE613 3.7 61.3 1.0 C5 A:PGE613 3.7 65.7 1.0 C6 A:PGE613 3.8 66.6 1.0 C1 A:PGE613 3.8 61.3 1.0 C3 A:PGE613 3.8 60.6 1.0 CD1 A:TYR314 4.4 45.0 1.0 CE1 A:TYR314 4.8 46.2 1.0

T.Skalova, J.Duskova, J.Hasek, A.Stepankova, T.Koval, L.H.Ostergaard, J.Dohnalek. Structure of Laccase From Streptomyces Coelicolor After Soaking with Potassium Hexacyanoferrate and at An Improved Resolution of 2.3 A Acta Crystallogr.,Sect.F V. 67 27 2011.
ISSN: ESSN 1744-3091
PubMed: 21206017
DOI: 10.1107/S1744309110046099