Sodium in PDB 3cx4: Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides

All present enzymatic activity of Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides:
2.4.1.21;

The structure of Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides, PDB code: 3cx4 was solved by F.Sheng, J.H.Geiger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	9.31 / 2.29
Space group	I 41
Cell size a, b, c (Å), α, β, γ (°)	126.324, 126.324, 152.334, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 22.1

The binding sites of Sodium atom in the Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides (pdb code 3cx4). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides, PDB code: 3cx4:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides within 5.0Å range: probe atom residue distance (Å) B Occ A:Na518 b:58.8 occ:1.00 O2B A:ADP500 3.0 49.2 1.0 N A:ARG300 3.0 60.6 1.0 O A:HOH629 3.1 57.6 1.0 CG A:ARG300 3.5 65.2 1.0 C8 A:ADP500 3.5 48.1 1.0 C5' A:ADP500 3.6 45.8 1.0 O3A A:ADP500 3.6 47.5 1.0 CB A:ARG300 3.6 63.9 1.0 NZ A:LYS305 3.7 47.0 1.0 CA A:SER299 3.8 54.4 1.0 PB A:ADP500 3.8 47.6 1.0 NE A:ARG300 3.8 66.8 1.0 C A:SER299 3.8 57.1 1.0 CA A:ARG300 3.9 63.8 1.0 CE A:LYS305 3.9 48.0 1.0 CD A:ARG300 4.2 65.7 1.0 OG A:SER299 4.2 53.0 1.0 N7 A:ADP500 4.2 48.1 1.0 O1B A:ADP500 4.4 47.6 1.0 O5' A:ADP500 4.5 47.2 1.0 O4' A:ADP500 4.5 46.4 1.0 CB A:SER299 4.5 53.4 1.0 PA A:ADP500 4.6 46.0 1.0 O2A A:ADP500 4.6 48.6 1.0 N9 A:ADP500 4.7 47.9 1.0 O A:VAL298 4.7 47.9 1.0 C4' A:ADP500 4.7 45.8 1.0 O A:ARG300 4.7 66.1 1.0 CD A:LYS305 4.8 47.2 1.0 C A:ARG300 4.8 65.2 1.0 N A:SER299 4.9 51.2 1.0 CZ A:ARG300 4.9 67.0 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of E.Coli Gs Mutant E377A in Complex with Adp and Oligosaccharides within 5.0Å range: probe atom residue distance (Å) B Occ A:Na519 b:58.4 occ:1.00 OH A:TYR469 2.5 42.9 1.0 OG1 A:THR209 2.6 45.2 1.0 O A:ALA210 2.9 44.8 1.0 CB A:THR209 3.3 44.3 1.0 C A:ALA210 3.4 45.3 1.0 O A:VAL244 3.5 45.6 1.0 CZ A:TYR469 3.5 42.1 1.0 CE2 A:TYR469 3.7 41.6 1.0 CB A:VAL244 3.8 45.8 1.0 N A:ALA210 3.9 44.9 1.0 O A:HOH545 3.9 42.0 1.0 CA A:VAL211 3.9 46.4 1.0 N A:VAL244 3.9 46.8 1.0 N A:VAL211 4.0 45.8 1.0 CB A:THR159 4.0 42.0 1.0 C A:THR209 4.0 44.4 1.0 OG1 A:THR159 4.1 43.1 1.0 CG1 A:VAL211 4.1 44.9 1.0 CA A:ALA210 4.2 44.3 1.0 CA A:VAL244 4.2 45.5 1.0 C A:VAL244 4.3 45.8 1.0 CA A:THR209 4.3 44.3 1.0 CG2 A:THR209 4.3 42.6 1.0 CG2 A:THR159 4.4 38.5 1.0 CG2 A:VAL244 4.4 45.2 1.0 O A:THR209 4.5 44.7 1.0 CB A:VAL211 4.7 46.5 1.0 CE1 A:TYR469 4.7 41.4 1.0 CG1 A:VAL244 4.9 44.4 1.0 C A:VAL211 4.9 47.2 1.0

F.Sheng, A.Yep, L.Feng, J.Preiss, J.H.Geiger. Oligosaccharide Binding in Escherichia Coli Glycogen Synthase. Biochemistry V. 48 10089 2009.
ISSN: ISSN 0006-2960
PubMed: 19761218
DOI: 10.1021/BI900916T