Atomistry » Sodium » PDB 3b2n-3biu » 3b35
Atomistry »
  Sodium »
    PDB 3b2n-3biu »
      3b35 »

Sodium in PDB 3b35: Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus

Enzymatic activity of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus

All present enzymatic activity of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus:
3.4.11.10;

Protein crystallography data

The structure of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus, PDB code: 3b35 was solved by N.J.Ataie, Q.Q.Hoang, G.A.Petsko, D.Ringe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 41.00 / 1.10
Space group P 61 2 2
Cell size a, b, c (Å), α, β, γ (°) 109.148, 109.148, 90.990, 90.00, 90.00, 120.00
R / Rfree (%) 14.9 / 16.6

Other elements in 3b35:

The structure of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus also contains other interesting chemical elements:

Zinc (Zn) 2 atoms

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus (pdb code 3b35). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 7 binding sites of Sodium where determined in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus, PDB code: 3b35:
Jump to Sodium binding site number: 1; 2; 3; 4; 5; 6; 7;

Sodium binding site 1 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 1 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na294

b:14.4
occ:1.00
 OG1 A:THR9 3.0 13.2 1.0
O A:HOH524 3.3 27.0 1.0
N A:THR9 3.4 11.1 1.0
CA A:GLN6 3.6 12.4 1.0
O A:HOH354 3.6 23.9 1.0
C A:GLN6 3.7 11.1 1.0
O A:GLN6 3.7 11.5 1.0
CB A:ALA8 3.7 12.4 1.0
OE1 A:GLN6 3.7 19.3 1.0
CB A:THR9 3.7 11.7 1.0
N A:ALA8 3.8 11.2 1.0
CB A:GLN6 4.1 12.9 1.0
O A:HOH608 4.1 27.5 1.0
CA A:ALA8 4.1 11.4 1.0
CA A:THR9 4.2 11.2 1.0
C A:ALA8 4.2 10.7 1.0
N A:GLN7 4.3 12.2 1.0
CD A:GLN6 4.3 17.4 1.0
O A:THR5 4.4 15.2 1.0
C A:GLN7 4.7 11.5 1.0
N A:GLN6 4.8 12.7 1.0
CG A:GLN6 4.8 15.4 1.0
O A:HOH530 4.8 28.2 1.0

Sodium binding site 2 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 2 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na295

b:14.3
occ:1.00
 OG A:SER27 2.6 11.3 1.0
O A:GLY24 2.7 10.9 1.0
OG A:SER28 2.8 12.9 1.0
N A:SER28 3.3 10.2 1.0
C A:GLY24 3.6 10.2 1.0
CB A:SER27 3.8 10.6 1.0
CB A:SER28 3.8 11.6 1.0
CA A:SER28 3.8 10.7 1.0
CA A:GLY24 3.9 10.9 1.0
C A:SER27 3.9 10.1 1.0
O A:HOH545 4.1 35.8 1.0
CA A:SER27 4.3 9.9 1.0
O A:HOH328 4.3 15.6 1.0
O A:SER27 4.7 11.0 1.0
N A:SER27 4.7 9.6 1.0
O A:THR23 4.8 10.7 1.0
N A:THR25 4.9 9.6 1.0

Sodium binding site 3 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 3 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na296

b:14.2
occ:1.00
 O A:HOH636 2.7 36.7 1.0
O A:HOH396 3.1 27.6 1.0
N A:ASN74 3.2 13.9 1.0
O A:HOH557 3.3 32.3 1.0
CA A:TYR73 3.6 15.2 1.0
CA A:THR38 3.6 13.1 1.0
ND2 A:ASN74 3.6 16.2 1.0
CG A:ASN74 3.7 14.6 1.0
CB A:THR38 3.8 13.7 1.0
O A:THR38 3.8 15.7 1.0
CB A:ASN74 3.9 14.0 1.0
CG2 A:THR38 3.9 14.0 1.0
C A:TYR73 3.9 14.1 1.0
O A:HOH359 3.9 20.0 1.0
CB A:TYR73 4.0 14.7 1.0
C A:THR38 4.1 12.8 1.0
O A:HOH326 4.1 13.6 1.0
CA A:ASN74 4.2 13.1 1.0
O A:TYR37 4.3 11.8 1.0
OD1 A:ASN74 4.3 15.2 1.0
O A:HOH602 4.6 24.2 1.0
O A:GLY72 4.6 22.0 1.0
N A:THR38 4.7 12.3 1.0
O A:HOH617 4.8 25.7 1.0
N A:TYR73 4.8 16.2 1.0
O A:HOH619 4.9 22.2 1.0
C A:TYR37 4.9 11.8 1.0

Sodium binding site 4 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 4 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 4 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na297

b:16.6
occ:1.00
 O A:HOH375 3.2 19.0 1.0
CG A:GLN54 3.8 14.2 1.0
CA A:ALA55 3.9 13.5 1.0
N A:ALA55 4.0 12.2 1.0
C A:GLN54 4.2 12.7 1.0
O A:GLN54 4.2 13.8 1.0
CB A:ALA55 4.3 14.4 1.0
CB A:GLN54 4.6 11.9 1.0
CB A:ALA58 4.7 23.9 1.0
O A:HOH473 4.8 27.2 1.0
NE2 A:GLN54 4.9 18.6 1.0
CD A:GLN54 4.9 15.4 1.0

Sodium binding site 5 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 5 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 5 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na298

b:9.9
occ:1.00
 O A:HOH411 3.2 18.6 1.0
N A:THR258 3.4 10.7 1.0
CA A:THR257 3.5 10.9 1.0
CB A:THR257 3.7 11.6 1.0
CG2 A:THR258 3.8 12.2 1.0
C A:THR257 3.9 10.2 1.0
O A:HOH657 4.1 16.5 0.5
CB A:ILE102 4.2 12.5 1.0
OG1 A:THR258 4.2 12.8 1.0
CG2 A:ILE102 4.3 12.8 1.0
CG2 A:THR257 4.3 12.2 1.0
CB A:THR258 4.3 11.0 1.0
CD1 A:ILE102 4.4 15.3 1.0
CA A:THR258 4.4 10.7 1.0
O A:HIS256 4.4 12.3 1.0
N A:THR257 4.8 10.7 1.0
CG1 A:ILE102 4.8 13.1 1.0
OG1 A:THR257 4.9 11.3 1.0

Sodium binding site 6 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 6 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 6 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na299

b:15.8
occ:1.00
 OG A:SER215 3.0 19.3 1.0
O A:HOH390 3.2 25.6 1.0
N A:SER215 3.3 14.3 1.0
C A:LEU213 3.4 12.1 1.0
CA A:LEU213 3.5 12.3 1.0
O A:HOH516 3.6 24.8 1.0
N A:PRO214 3.6 13.1 1.0
CB A:SER215 3.6 16.8 1.0
CD A:PRO214 3.8 13.9 1.0
O A:LEU213 3.9 12.1 1.0
CA A:SER215 3.9 15.4 1.0
CB A:LEU213 4.2 12.0 1.0
CD2 A:LEU213 4.2 14.4 1.0
N A:LEU216 4.3 12.9 1.0
C A:PRO214 4.3 14.0 1.0
CG A:LEU216 4.4 12.5 1.0
CG A:PRO214 4.4 15.0 1.0
C A:SER215 4.5 14.2 1.0
CA A:PRO214 4.5 14.1 1.0
O A:TYR212 4.6 12.3 1.0
N A:LEU213 4.7 11.3 1.0
CD2 A:LEU216 4.8 14.4 1.0
CG A:LEU213 4.9 12.7 1.0

Sodium binding site 7 out of 7 in 3b35

Go back to Sodium Binding Sites List in 3b35
Sodium binding site 7 out of 7 in the Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 7 of Crystal Structure of the M180A Mutant of the Aminopeptidase From Vibrio Proteolyticus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na300

b:14.8
occ:1.00
 NZ A:LYS273 3.1 18.9 1.0
O A:HOH459 3.1 23.0 1.0
N A:ALA19 3.2 11.0 1.0
O A:HOH431 3.6 25.1 1.0
CB A:ALA19 3.8 11.7 1.0
O A:VAL17 3.9 12.1 1.0
CE A:LYS273 3.9 13.4 1.0
CA A:ASP18 4.0 11.2 1.0
CD A:LYS273 4.0 13.4 1.0
C A:ASP18 4.1 10.7 1.0
CA A:ALA19 4.1 10.8 1.0
OD1 A:ASP18 4.2 16.1 1.0
C A:VAL17 4.8 10.4 1.0
O A:HOH590 4.8 23.1 1.0
N A:ASP18 4.8 11.0 1.0
CG A:ASP18 4.9 14.2 1.0
N A:SER20 4.9 10.3 1.0
CB A:ASP18 5.0 12.5 1.0

Reference:

N.J.Ataie, Q.Q.Hoang, M.P.Zahniser, Y.Tu, A.Milne, G.A.Petsko, D.Ringe. Zinc Coordination Geometry and Ligand Binding Affinity: the Structural and Kinetic Analysis of the Second-Shell Serine 228 Residue and the Methionine 180 Residue of the Aminopeptidase From Vibrio Proteolyticus. Biochemistry V. 47 7673 2008.
ISSN: ISSN 0006-2960
PubMed: 18576673
DOI: 10.1021/BI702188E
Page generated: Mon Oct 7 05:57:59 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy