Sodium in PDB 2zal: Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate

All present enzymatic activity of Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate:
3.4.19.5; 3.5.1.1;

The structure of Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate, PDB code: 2zal was solved by K.Michalska, K.Brzezinski, M.Jaskolski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	49.890, 77.280, 147.530, 90.00, 90.00, 90.00
R / Rfree (%)	15.7 / 18.8

The structure of Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Calcium	(Ca)	5 atoms

The binding sites of Sodium atom in the Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate (pdb code 2zal). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate, PDB code: 2zal:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate within 5.0Å range: probe atom residue distance (Å) B Occ A:Na800 b:17.3 occ:1.00 O A:ILE70 2.2 10.4 1.0 O A:CYS63 2.3 10.2 1.0 O A:ALA68 2.3 10.5 1.0 O A:GLU61 2.5 13.7 1.0 O A:PHE66 2.6 11.6 1.0 O A:LEU60 2.7 11.8 1.0 C A:GLU61 3.2 12.9 1.0 C A:CYS63 3.3 12.2 1.0 C A:ILE70 3.4 12.2 1.0 C A:ALA68 3.5 11.5 1.0 CA A:GLU61 3.5 12.6 1.0 C A:PHE66 3.7 11.9 1.0 C A:LEU60 3.7 11.4 1.0 N A:CYS63 3.9 13.3 1.0 N A:ILE70 3.9 11.9 1.0 N A:ALA68 4.0 11.9 1.0 N A:GLU61 4.1 11.5 1.0 N A:PHE66 4.1 11.7 1.0 CA A:CYS63 4.2 13.1 1.0 N A:PRO64 4.2 12.4 1.0 CA A:ILE70 4.2 12.6 1.0 CA A:PHE66 4.2 12.0 1.0 CB A:PHE66 4.2 12.6 1.0 C A:GLY69 4.2 11.6 1.0 N A:GLU62 4.2 13.8 1.0 CA A:PRO64 4.3 12.9 1.0 C A:GLU62 4.3 13.9 1.0 CA A:ALA68 4.3 12.1 1.0 N A:GLY71 4.4 11.4 1.0 CA A:GLY71 4.5 11.2 1.0 N A:GLY69 4.5 11.5 1.0 CA A:GLY69 4.6 11.1 1.0 C A:PRO64 4.6 12.7 1.0 CB A:ALA68 4.7 12.4 1.0 O A:GLY69 4.7 11.8 1.0 CB A:ILE70 4.7 13.2 1.0 O A:GLU62 4.7 13.7 1.0 N A:ASN67 4.7 11.5 1.0 CA A:GLU62 4.8 13.9 1.0 CB A:CYS63 4.8 13.4 1.0 CB A:GLU61 4.9 13.2 1.0 C A:GLY71 4.9 11.2 1.0 N A:LEU65 5.0 12.3 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of E. Coli Isoaspartyl Aminopeptidase/L-Asparaginase in Complex with L-Aspartate within 5.0Å range: probe atom residue distance (Å) B Occ C:Na801 b:20.3 occ:1.00 O C:ILE70 2.2 9.9 1.0 O C:CYS63 2.3 9.6 1.0 O C:ALA68 2.4 11.0 1.0 O C:GLU61 2.4 11.8 1.0 O C:PHE66 2.7 9.8 1.0 O C:LEU60 2.7 12.1 1.0 C C:GLU61 3.2 11.6 1.0 C C:CYS63 3.3 11.0 1.0 C C:ILE70 3.4 10.6 1.0 C C:ALA68 3.6 11.2 1.0 CA C:GLU61 3.6 11.6 1.0 C C:PHE66 3.7 10.3 1.0 C C:LEU60 3.8 12.4 1.0 N C:CYS63 3.8 11.1 1.0 N C:ILE70 3.9 10.4 1.0 N C:ALA68 4.1 11.1 1.0 CB C:PHE66 4.1 10.6 1.0 N C:PHE66 4.1 9.9 1.0 N C:PRO64 4.1 11.4 1.0 CA C:ILE70 4.1 11.0 1.0 CA C:CYS63 4.2 11.6 1.0 N C:GLU61 4.2 11.5 1.0 CA C:PRO64 4.2 11.7 1.0 C C:GLY69 4.2 10.3 1.0 CA C:PHE66 4.2 9.8 1.0 N C:GLU62 4.2 11.5 1.0 C C:GLU62 4.3 12.2 1.0 CA C:ALA68 4.4 11.3 1.0 N C:GLY71 4.4 10.6 1.0 CB C:ILE70 4.5 11.0 1.0 N C:GLY69 4.5 10.4 1.0 C C:PRO64 4.6 11.2 1.0 CA C:GLY71 4.6 10.7 1.0 CA C:GLY69 4.6 10.3 1.0 O C:GLY69 4.6 9.4 1.0 CB C:ALA68 4.7 11.5 1.0 CA C:GLU62 4.8 12.0 1.0 O C:GLU62 4.8 12.3 1.0 N C:ASN67 4.8 10.3 1.0 CB C:CYS63 4.8 11.8 1.0 N C:LEU65 4.9 10.9 1.0 C C:GLY71 5.0 11.0 1.0 CB C:GLU61 5.0 11.2 1.0 O C:PRO64 5.0 11.6 1.0

K.Michalska, K.Brzezinski, M.Jaskolski. Crystal Structure of Isoaspartyl Aminopeptidase in Complex with L-Aspartate J.Biol.Chem. V. 280 28484 2005.
ISSN: ISSN 0021-9258
PubMed: 15946951
DOI: 10.1074/JBC.M504501200