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Sodium in PDB 1z45: Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose

Enzymatic activity of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose

All present enzymatic activity of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose:
5.1.3.2; 5.1.3.3;

Protein crystallography data

The structure of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose, PDB code: 1z45 was solved by J.B.Thoden, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.85
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 120.900, 125.200, 142.300, 90.00, 90.00, 90.00
R / Rfree (%) n/a / n/a

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose (pdb code 1z45). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose, PDB code: 1z45:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 1z45

Go back to Sodium Binding Sites List in 1z45
Sodium binding site 1 out of 2 in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na701

b:38.0
occ:1.00
 O A:LEU357 2.3 16.7 1.0
O A:VAL360 2.4 17.8 1.0
O A:HOH1054 2.5 38.7 1.0
O A:HOH1053 2.5 32.9 1.0
O A:HOH1223 2.5 47.7 1.0
O A:HOH1055 2.5 43.7 1.0
C A:LEU357 3.4 17.0 1.0
C A:VAL360 3.5 32.0 1.0
N A:LEU357 3.9 21.4 1.0
N A:VAL360 4.0 16.4 1.0
CA A:VAL360 4.1 19.3 1.0
CB A:VAL360 4.2 19.8 1.0
CA A:LEU357 4.2 19.8 1.0
OE2 A:GLU361 4.2 53.2 1.0
OE1 A:GLN356 4.3 62.7 1.0
N A:ARG358 4.3 15.2 1.0
CA A:ARG358 4.4 18.7 1.0
O A:HOH1405 4.5 52.3 1.0
CD A:GLU361 4.5 0.0 1.0
O A:HOH1276 4.5 56.9 1.0
N A:GLU361 4.6 19.2 1.0
OE1 A:GLU361 4.6 59.8 1.0
N A:GLY359 4.7 23.3 1.0
CG1 A:VAL360 4.7 15.6 1.0
C A:ARG358 4.8 28.6 1.0
O A:HOH882 4.8 33.3 1.0
CA A:GLU361 4.8 17.0 1.0
C A:GLN356 4.9 22.2 1.0
CB A:LEU357 4.9 19.7 1.0

Sodium binding site 2 out of 2 in 1z45

Go back to Sodium Binding Sites List in 1z45
Sodium binding site 2 out of 2 in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na702

b:51.5
occ:1.00
 OE1 A:GLN475 2.3 23.1 1.0
O A:HOH817 2.3 16.7 1.0
O A:GLU350 2.4 23.7 1.0
O A:HOH816 2.4 26.5 1.0
OD1 A:ASN351 2.8 20.3 1.0
O A:HOH1106 3.0 31.9 1.0
C A:GLU350 3.4 25.7 1.0
O A:HOH819 3.4 22.6 1.0
CD A:GLN475 3.4 25.3 1.0
CG A:ASN351 3.5 21.5 1.0
O A:HOH815 3.8 45.5 1.0
CB A:GLN475 3.9 12.3 1.0
CA A:ASN351 4.0 22.2 1.0
N A:ASN351 4.1 21.1 1.0
CB A:ASN351 4.2 14.2 1.0
ND2 A:ASN351 4.2 13.7 1.0
O A:HOH786 4.2 23.7 1.0
CG A:GLN475 4.3 28.1 1.0
NE2 A:GLN475 4.3 20.2 1.0
CA A:GLU350 4.3 15.9 1.0
CA A:GLN475 4.4 10.2 1.0
O A:HOH822 4.4 29.9 1.0
N A:ASN476 4.5 22.0 1.0
NH2 A:ARG54 4.5 20.7 1.0
CB A:GLU350 4.5 17.0 1.0
O A:HOH814 4.6 32.8 1.0
O A:HOH1105 4.7 29.6 1.0
O A:HOH821 4.9 17.9 1.0
OE1 A:GLU350 5.0 41.3 1.0

Reference:

J.B.Thoden, H.M.Holden. The Molecular Architecture of Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae. J.Biol.Chem. V. 280 21900 2005.
ISSN: ISSN 0021-9258
PubMed: 15795221
DOI: 10.1074/JBC.M502411200
Page generated: Tue Dec 15 05:44:18 2020

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