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Sodium in PDB 5iio: Crystal Structure of the Dna Polymerase Lambda Binary Complex

Enzymatic activity of Crystal Structure of the Dna Polymerase Lambda Binary Complex

All present enzymatic activity of Crystal Structure of the Dna Polymerase Lambda Binary Complex:
2.7.7.7;

Protein crystallography data

The structure of Crystal Structure of the Dna Polymerase Lambda Binary Complex, PDB code: 5iio was solved by M.J.Burak, K.E.Guja, M.Garcia-Diaz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.09 / 2.08
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 191.423, 98.980, 105.034, 90.00, 90.00, 90.00
R / Rfree (%) 19.1 / 23.5

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of the Dna Polymerase Lambda Binary Complex (pdb code 5iio). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 6 binding sites of Sodium where determined in the Crystal Structure of the Dna Polymerase Lambda Binary Complex, PDB code: 5iio:
Jump to Sodium binding site number: 1; 2; 3; 4; 5; 6;

Sodium binding site 1 out of 6 in 5iio

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Sodium binding site 1 out of 6 in the Crystal Structure of the Dna Polymerase Lambda Binary Complex


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of the Dna Polymerase Lambda Binary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na601

b:35.6
occ:1.00
O A:SER339 2.3 36.8 1.0
O A:ALA344 2.3 31.0 1.0
O C:HOH115 2.4 36.5 1.0
OP1 C:DA5 2.5 25.8 1.0
O C:HOH107 2.5 36.4 1.0
O A:ILE341 2.6 31.4 1.0
C A:SER339 3.3 36.7 1.0
C A:ALA344 3.4 32.2 1.0
C A:ILE341 3.4 32.0 1.0
O A:HOH788 3.6 36.0 1.0
P C:DA5 3.6 29.1 1.0
N A:ALA344 3.8 29.8 1.0
O C:HOH118 3.8 43.1 1.0
OP2 C:DA5 3.8 36.5 1.0
CA A:SER339 3.9 33.4 1.0
N A:ILE341 4.0 33.4 1.0
N A:GLY343 4.1 27.2 1.0
CA A:ALA344 4.1 33.9 1.0
CA A:TRP342 4.1 33.9 1.0
N A:TRP342 4.1 32.2 1.0
N A:ASN340 4.2 31.1 1.0
C A:ASN340 4.3 35.2 1.0
N A:GLY345 4.3 32.4 1.0
CA A:ILE341 4.3 34.9 1.0
CB A:SER339 4.4 37.4 1.0
C A:TRP342 4.5 29.1 1.0
CA A:ASN340 4.5 36.1 1.0
O3' C:DT4 4.6 32.2 1.0
CA A:GLY345 4.6 32.4 1.0
CB A:ALA344 4.7 31.9 1.0
C A:GLY343 4.7 35.9 1.0
O5' C:DA5 4.7 34.0 1.0
O A:ASN340 4.9 38.9 1.0
O A:HOH902 5.0 38.3 1.0
CA A:GLY343 5.0 33.5 1.0
O A:PHE338 5.0 32.8 1.0

Sodium binding site 2 out of 6 in 5iio

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Sodium binding site 2 out of 6 in the Crystal Structure of the Dna Polymerase Lambda Binary Complex


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of the Dna Polymerase Lambda Binary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na602

b:46.5
occ:1.00
O A:HOH931 2.3 53.6 1.0
O A:ILE302 2.5 47.8 1.0
O A:ILE305 2.8 42.2 1.0
O A:CYS300 2.8 43.6 1.0
OP1 D:DC3 2.8 50.5 1.0
C A:ILE302 3.3 48.7 1.0
C A:ILE305 3.7 45.1 1.0
P D:DC3 3.8 50.9 1.0
OP2 D:DC3 3.8 44.8 1.0
CA A:PRO303 3.8 50.1 1.0
C A:CYS300 3.8 45.5 1.0
N A:PRO303 3.8 49.9 1.0
N A:ILE305 3.8 43.7 1.0
N A:GLY304 4.0 47.4 1.0
N A:ILE302 4.2 46.3 1.0
CA A:ILE305 4.3 44.0 1.0
C A:PRO303 4.3 50.2 1.0
C A:SER301 4.3 49.9 1.0
CA A:ILE302 4.3 45.5 1.0
O A:SER301 4.5 52.0 1.0
CA A:CYS300 4.5 44.4 1.0
N A:GLY306 4.6 39.1 1.0
N A:SER301 4.7 44.9 1.0
O3' D:DC2 4.7 48.6 1.0
CB A:ILE305 4.8 42.4 1.0
CA A:SER301 4.8 44.9 1.0
C A:GLY304 4.9 47.8 1.0
CA A:GLY306 4.9 41.3 1.0
O5' D:DC3 5.0 47.9 1.0

Sodium binding site 3 out of 6 in 5iio

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Sodium binding site 3 out of 6 in the Crystal Structure of the Dna Polymerase Lambda Binary Complex


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Crystal Structure of the Dna Polymerase Lambda Binary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Na601

b:40.0
occ:1.00
OP1 G:DA5 2.4 34.1 1.0
O E:ILE341 2.4 35.8 1.0
O E:ALA344 2.5 35.2 1.0
O E:SER339 2.5 44.7 1.0
O G:HOH101 2.6 42.3 1.0
O G:HOH107 2.6 47.3 1.0
C E:ALA344 3.4 37.9 1.0
C E:ILE341 3.4 44.5 1.0
C E:SER339 3.4 45.0 1.0
P G:DA5 3.5 39.7 1.0
N E:ALA344 3.6 36.9 1.0
OP2 G:DA5 3.8 38.7 1.0
N E:GLY343 3.8 37.0 1.0
O E:HOH773 3.8 43.7 1.0
CA E:TRP342 4.0 41.9 1.0
CA E:SER339 4.0 45.9 1.0
CA E:ALA344 4.0 42.4 1.0
N E:TRP342 4.1 44.4 1.0
N E:ILE341 4.1 41.4 1.0
C E:TRP342 4.3 42.0 1.0
N E:GLY345 4.3 36.0 1.0
N E:ASN340 4.4 44.0 1.0
CA E:ILE341 4.4 41.3 1.0
C E:ASN340 4.4 44.5 1.0
O3' G:DT4 4.5 39.8 1.0
CB E:SER339 4.5 39.6 1.0
CB E:ALA344 4.6 32.8 1.0
C E:GLY343 4.6 38.0 1.0
CA E:ASN340 4.6 48.6 1.0
O5' G:DA5 4.6 41.7 1.0
CA E:GLY345 4.7 35.5 1.0
CA E:GLY343 4.8 41.1 1.0

Sodium binding site 4 out of 6 in 5iio

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Sodium binding site 4 out of 6 in the Crystal Structure of the Dna Polymerase Lambda Binary Complex


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 4 of Crystal Structure of the Dna Polymerase Lambda Binary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Na601

b:33.6
occ:1.00
O I:SER339 2.3 33.6 1.0
OP1 K:DA5 2.4 33.2 1.0
O I:ALA344 2.4 31.4 1.0
O K:HOH110 2.5 36.2 1.0
O I:ILE341 2.6 31.9 1.0
O K:HOH113 2.6 40.1 1.0
C I:SER339 3.3 36.9 1.0
O I:HOH747 3.4 31.0 1.0
C I:ALA344 3.5 33.5 1.0
P K:DA5 3.5 34.3 1.0
C I:ILE341 3.5 33.9 1.0
OP2 K:DA5 3.7 35.4 1.0
CA I:SER339 3.8 35.2 1.0
N I:ALA344 3.8 34.2 1.0
N I:GLY343 4.1 33.6 1.0
N I:ILE341 4.1 36.5 1.0
CA I:ALA344 4.2 34.0 1.0
CA I:TRP342 4.2 34.1 1.0
O K:HOH119 4.2 49.9 1.0
N I:TRP342 4.2 32.4 1.0
CB I:SER339 4.3 36.8 1.0
N I:ASN340 4.3 36.4 1.0
C I:ASN340 4.4 36.4 1.0
N I:GLY345 4.4 33.3 1.0
CA I:ILE341 4.5 37.4 1.0
O3' K:DT4 4.5 33.3 1.0
C I:TRP342 4.5 37.7 1.0
CA I:GLY345 4.6 33.2 1.0
O5' K:DA5 4.6 37.6 1.0
CA I:ASN340 4.6 32.8 1.0
C I:GLY343 4.7 33.8 1.0
CB I:ALA344 4.7 32.8 1.0
O I:ASN340 4.9 37.7 1.0
O I:HOH840 4.9 47.0 1.0
O I:PHE338 4.9 32.3 1.0
CA I:GLY343 5.0 32.9 1.0

Sodium binding site 5 out of 6 in 5iio

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Sodium binding site 5 out of 6 in the Crystal Structure of the Dna Polymerase Lambda Binary Complex


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 5 of Crystal Structure of the Dna Polymerase Lambda Binary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
M:Na601

b:24.6
occ:1.00
O M:ALA344 2.4 24.9 1.0
OP1 O:DA5 2.4 24.4 1.0
O M:SER339 2.4 25.4 1.0
O M:HOH962 2.5 28.4 1.0
O M:ILE341 2.5 24.3 1.0
O O:HOH108 2.6 27.6 1.0
C M:ALA344 3.4 25.5 1.0
C M:SER339 3.4 25.8 1.0
C M:ILE341 3.5 23.2 1.0
P O:DA5 3.5 23.8 1.0
O M:HOH801 3.6 20.0 1.0
N M:ALA344 3.7 24.0 1.0
OP2 O:DA5 3.7 27.8 1.0
CA M:SER339 4.0 24.0 1.0
N M:GLY343 4.0 24.2 1.0
O O:HOH121 4.0 40.4 1.0
CA M:TRP342 4.1 25.4 1.0
CA M:ALA344 4.1 25.0 1.0
N M:ILE341 4.1 21.0 1.0
N M:TRP342 4.2 24.9 1.0
N M:GLY345 4.3 22.8 1.0
C M:ASN340 4.3 24.0 1.0
C M:TRP342 4.4 26.3 1.0
CA M:ILE341 4.4 21.8 1.0
N M:ASN340 4.4 26.6 1.0
CB M:SER339 4.5 23.9 1.0
O3' O:DT4 4.5 23.1 1.0
CA M:GLY345 4.5 25.0 1.0
CB M:ALA344 4.6 18.1 1.0
CA M:ASN340 4.6 25.7 1.0
O5' O:DA5 4.6 24.9 1.0
C M:GLY343 4.7 29.4 1.0
O M:ASN340 4.8 25.5 1.0
CA M:GLY343 4.9 21.7 1.0
O M:HOH899 4.9 33.8 1.0
O M:PHE338 5.0 22.9 1.0

Sodium binding site 6 out of 6 in 5iio

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Sodium binding site 6 out of 6 in the Crystal Structure of the Dna Polymerase Lambda Binary Complex


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 6 of Crystal Structure of the Dna Polymerase Lambda Binary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
M:Na602

b:34.9
occ:1.00
O M:HOH948 2.2 37.0 1.0
O M:ILE305 2.3 29.3 1.0
O P:HOH110 2.5 46.2 1.0
O M:ILE302 2.5 29.7 1.0
O M:CYS300 2.8 39.2 1.0
OP1 P:DC3 2.8 35.5 1.0
C M:ILE305 3.4 32.2 1.0
C M:ILE302 3.5 32.7 1.0
P P:DC3 3.6 38.5 1.0
OP2 P:DC3 3.7 38.5 1.0
C M:CYS300 3.7 37.1 1.0
N M:ILE305 3.8 30.8 1.0
O P:HOH104 3.9 45.0 1.0
CA M:PRO303 4.1 30.6 1.0
CA M:ILE305 4.1 32.7 1.0
N M:PRO303 4.1 34.8 1.0
N M:ILE302 4.1 34.5 1.0
N M:GLY304 4.2 30.6 1.0
CA M:CYS300 4.3 36.1 1.0
N M:GLY306 4.4 34.9 1.0
C M:SER301 4.4 34.8 1.0
CA M:ILE302 4.4 32.6 1.0
O3' P:DC2 4.5 32.8 1.0
C M:PRO303 4.5 32.7 1.0
CB M:ILE305 4.6 31.0 1.0
N M:SER301 4.6 34.9 1.0
CA M:GLY306 4.7 37.6 1.0
O M:ALA299 4.7 35.5 1.0
CA M:SER301 4.8 34.6 1.0
C M:GLY304 4.8 35.3 1.0
O M:HOH1005 4.9 41.8 1.0
O M:SER301 4.9 32.8 1.0
O5' P:DC3 5.0 35.5 1.0

Reference:

M.J.Burak, K.E.Guja, E.Hambardjieva, B.Derkunt, M.Garcia-Diaz. A Fidelity Mechanism in Dna Polymerase Lambda Promotes Error-Free Bypass of 8-Oxo-Dg. Embo J. V. 35 2045 2016.
ISSN: ESSN 1460-2075
PubMed: 27481934
DOI: 10.15252/EMBJ.201694332
Page generated: Mon Oct 7 21:37:28 2024

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