Sodium in PDB 1xsl: Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
All present enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap:
2.7.7.7;
Protein crystallography data
The structure of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap, PDB code: 1xsl
was solved by
M.Garcia-Diaz,
K.Bebenek,
J.M.Krahn,
T.A.Kunkel,
L.C.Pedersen,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
28.47 /
2.30
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
191.504,
98.812,
104.519,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
20.7 /
25.2
|
Other elements in 1xsl:
The structure of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap also contains other interesting chemical elements:
Sodium Binding Sites:
The binding sites of Sodium atom in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
(pdb code 1xsl). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 6 binding sites of Sodium where determined in the
Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap, PDB code: 1xsl:
Jump to Sodium binding site number:
1;
2;
3;
4;
5;
6;
Sodium binding site 1 out
of 6 in 1xsl
Go back to
Sodium Binding Sites List in 1xsl
Sodium binding site 1 out
of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Na965
b:52.8
occ:1.00
|
O
|
J:HOH642
|
2.2
|
36.7
|
1.0
|
O
|
L:HOH645
|
2.3
|
46.0
|
1.0
|
O
|
J:HOH641
|
2.4
|
57.5
|
1.0
|
O
|
J:HOH643
|
2.4
|
59.8
|
1.0
|
O
|
J:HOH644
|
2.6
|
45.3
|
1.0
|
O
|
L:HOH640
|
2.6
|
52.5
|
1.0
|
N7
|
J:DG2
|
4.1
|
50.3
|
1.0
|
O
|
J:HOH428
|
4.2
|
38.3
|
1.0
|
O6
|
J:DG3
|
4.4
|
39.8
|
1.0
|
O6
|
J:DG2
|
4.4
|
47.3
|
1.0
|
N4
|
L:DC2
|
4.9
|
39.7
|
1.0
|
C5
|
J:DG2
|
4.9
|
48.9
|
1.0
|
O6
|
L:DG1
|
5.0
|
46.7
|
1.0
|
|
Sodium binding site 2 out
of 6 in 1xsl
Go back to
Sodium Binding Sites List in 1xsl
Sodium binding site 2 out
of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Na966
b:53.3
occ:1.00
|
O
|
N:HOH943
|
2.0
|
36.6
|
1.0
|
O
|
N:HOH945
|
2.2
|
43.7
|
1.0
|
O
|
P:HOH940
|
2.3
|
53.8
|
1.0
|
O
|
N:HOH944
|
2.4
|
56.2
|
1.0
|
O6
|
N:DG3
|
4.1
|
29.1
|
1.0
|
O6
|
P:DG1
|
4.2
|
33.8
|
1.0
|
O
|
N:HOH379
|
4.3
|
37.5
|
1.0
|
N4
|
P:DC2
|
4.4
|
29.9
|
1.0
|
O6
|
N:DG2
|
4.5
|
38.4
|
1.0
|
N7
|
N:DG2
|
4.5
|
38.0
|
1.0
|
|
Sodium binding site 3 out
of 6 in 1xsl
Go back to
Sodium Binding Sites List in 1xsl
Sodium binding site 3 out
of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na961
b:30.8
occ:1.00
|
OP1
|
C:DG5
|
2.5
|
24.3
|
1.0
|
O
|
A:SER339
|
2.5
|
30.4
|
1.0
|
O
|
C:HOH8
|
2.6
|
30.5
|
1.0
|
O
|
A:ALA344
|
2.6
|
26.0
|
1.0
|
O
|
A:ILE341
|
2.7
|
28.2
|
1.0
|
O
|
C:HOH156
|
2.8
|
29.3
|
1.0
|
O
|
A:HOH1014
|
3.4
|
43.3
|
1.0
|
C
|
A:SER339
|
3.4
|
30.5
|
1.0
|
P
|
C:DG5
|
3.5
|
25.1
|
1.0
|
C
|
A:ILE341
|
3.6
|
30.2
|
1.0
|
C
|
A:ALA344
|
3.6
|
25.7
|
1.0
|
OP2
|
C:DG5
|
3.7
|
23.8
|
1.0
|
N
|
A:ALA344
|
3.9
|
26.2
|
1.0
|
CA
|
A:SER339
|
4.0
|
31.7
|
1.0
|
N
|
A:GLY343
|
4.1
|
27.4
|
1.0
|
N
|
A:ILE341
|
4.2
|
30.8
|
1.0
|
O
|
C:HOH905
|
4.2
|
62.9
|
1.0
|
CA
|
A:TRP342
|
4.2
|
29.8
|
1.0
|
N
|
A:TRP342
|
4.3
|
30.6
|
1.0
|
CA
|
A:ALA344
|
4.3
|
26.3
|
1.0
|
C
|
A:ASN340
|
4.4
|
31.9
|
1.0
|
CB
|
A:SER339
|
4.4
|
29.4
|
1.0
|
N
|
A:ASN340
|
4.5
|
31.1
|
1.0
|
O3'
|
C:DC4
|
4.5
|
23.4
|
1.0
|
CA
|
A:ILE341
|
4.5
|
30.4
|
1.0
|
N
|
A:GLY345
|
4.6
|
25.7
|
1.0
|
C
|
A:TRP342
|
4.6
|
29.8
|
1.0
|
O5'
|
C:DG5
|
4.7
|
24.8
|
1.0
|
CA
|
A:ASN340
|
4.7
|
31.4
|
1.0
|
CA
|
A:GLY345
|
4.7
|
27.5
|
1.0
|
CB
|
A:ALA344
|
4.8
|
23.6
|
1.0
|
C
|
A:GLY343
|
4.9
|
26.3
|
1.0
|
O
|
C:HOH53
|
4.9
|
23.9
|
1.0
|
O
|
A:ASN340
|
4.9
|
33.8
|
1.0
|
|
Sodium binding site 4 out
of 6 in 1xsl
Go back to
Sodium Binding Sites List in 1xsl
Sodium binding site 4 out
of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 4 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Na962
b:55.3
occ:1.00
|
OP1
|
G:DG5
|
2.6
|
39.0
|
1.0
|
O
|
E:SER339
|
2.6
|
47.7
|
1.0
|
O
|
E:ILE341
|
2.7
|
44.6
|
1.0
|
O
|
E:ALA344
|
2.8
|
40.4
|
1.0
|
O
|
E:HOH1016
|
2.8
|
51.6
|
1.0
|
O
|
G:HOH508
|
2.9
|
42.6
|
1.0
|
C
|
E:ILE341
|
3.4
|
45.4
|
1.0
|
C
|
E:SER339
|
3.4
|
48.1
|
1.0
|
P
|
G:DG5
|
3.8
|
41.8
|
1.0
|
C
|
E:ALA344
|
3.8
|
40.7
|
1.0
|
N
|
E:ILE341
|
3.9
|
45.9
|
1.0
|
N
|
E:TRP342
|
3.9
|
43.6
|
1.0
|
CA
|
E:TRP342
|
3.9
|
43.6
|
1.0
|
N
|
E:ALA344
|
3.9
|
40.7
|
1.0
|
C
|
E:ASN340
|
4.0
|
48.0
|
1.0
|
N
|
E:GLY343
|
4.1
|
41.3
|
1.0
|
OP2
|
G:DG5
|
4.1
|
38.2
|
1.0
|
CA
|
E:SER339
|
4.1
|
49.1
|
1.0
|
N
|
E:ASN340
|
4.2
|
47.9
|
1.0
|
CA
|
E:ILE341
|
4.3
|
45.0
|
1.0
|
CA
|
E:ALA344
|
4.3
|
41.4
|
1.0
|
C
|
E:TRP342
|
4.4
|
43.6
|
1.0
|
CA
|
E:ASN340
|
4.4
|
47.9
|
1.0
|
O
|
E:ASN340
|
4.5
|
49.5
|
1.0
|
O5'
|
G:DG5
|
4.6
|
39.8
|
1.0
|
CB
|
E:ALA344
|
4.7
|
40.8
|
1.0
|
CB
|
E:SER339
|
4.7
|
47.5
|
1.0
|
N
|
E:GLY345
|
4.8
|
40.0
|
1.0
|
C
|
E:GLY343
|
4.9
|
41.9
|
1.0
|
O3'
|
G:DC4
|
5.0
|
41.2
|
1.0
|
|
Sodium binding site 5 out
of 6 in 1xsl
Go back to
Sodium Binding Sites List in 1xsl
Sodium binding site 5 out
of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 5 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Na963
b:53.3
occ:1.00
|
O
|
K:HOH303
|
2.5
|
39.4
|
1.0
|
O
|
I:SER339
|
2.5
|
34.4
|
1.0
|
OP1
|
K:DG5
|
2.5
|
36.1
|
1.0
|
O
|
K:HOH875
|
2.7
|
49.8
|
1.0
|
O
|
I:ALA344
|
2.8
|
33.8
|
1.0
|
O
|
I:ILE341
|
2.8
|
39.9
|
1.0
|
C
|
I:ILE341
|
3.6
|
38.7
|
1.0
|
P
|
K:DG5
|
3.6
|
33.6
|
1.0
|
C
|
I:SER339
|
3.6
|
35.6
|
1.0
|
OP2
|
K:DG5
|
3.8
|
35.3
|
1.0
|
C
|
I:ALA344
|
3.8
|
34.9
|
1.0
|
CA
|
I:TRP342
|
4.0
|
37.8
|
1.0
|
N
|
I:ALA344
|
4.1
|
33.8
|
1.0
|
N
|
I:TRP342
|
4.1
|
38.3
|
1.0
|
N
|
I:GLY343
|
4.1
|
34.0
|
1.0
|
N
|
I:ILE341
|
4.2
|
36.7
|
1.0
|
CA
|
I:SER339
|
4.3
|
36.6
|
1.0
|
C
|
I:ASN340
|
4.4
|
36.4
|
1.0
|
O5'
|
K:DG5
|
4.4
|
35.1
|
1.0
|
C
|
I:TRP342
|
4.5
|
36.4
|
1.0
|
CA
|
I:ALA344
|
4.5
|
34.9
|
1.0
|
CA
|
I:ILE341
|
4.5
|
37.6
|
1.0
|
N
|
I:ASN340
|
4.6
|
34.9
|
1.0
|
CA
|
I:ASN340
|
4.7
|
36.0
|
1.0
|
O
|
I:ASN340
|
4.7
|
37.5
|
1.0
|
N
|
I:GLY345
|
4.8
|
35.0
|
1.0
|
CB
|
I:SER339
|
4.8
|
36.6
|
1.0
|
O3'
|
K:DC4
|
4.9
|
36.5
|
1.0
|
CA
|
I:GLY345
|
4.9
|
35.2
|
1.0
|
CB
|
I:ALA344
|
5.0
|
36.0
|
1.0
|
|
Sodium binding site 6 out
of 6 in 1xsl
Go back to
Sodium Binding Sites List in 1xsl
Sodium binding site 6 out
of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 6 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
M:Na964
b:29.6
occ:1.00
|
OP1
|
O:DG5
|
2.3
|
20.6
|
1.0
|
O
|
M:SER339
|
2.4
|
26.9
|
1.0
|
O
|
M:ALA344
|
2.4
|
20.4
|
1.0
|
O
|
M:ILE341
|
2.6
|
27.8
|
1.0
|
O
|
O:HOH39
|
2.6
|
21.9
|
1.0
|
O
|
M:HOH978
|
2.7
|
26.6
|
1.0
|
C
|
M:ALA344
|
3.4
|
22.5
|
1.0
|
C
|
M:SER339
|
3.4
|
25.8
|
1.0
|
O
|
M:HOH1071
|
3.5
|
36.6
|
1.0
|
C
|
M:ILE341
|
3.5
|
26.4
|
1.0
|
P
|
O:DG5
|
3.5
|
22.6
|
1.0
|
N
|
M:ALA344
|
3.6
|
22.7
|
1.0
|
OP2
|
O:DG5
|
3.8
|
23.5
|
1.0
|
N
|
M:GLY343
|
3.9
|
24.4
|
1.0
|
CA
|
M:TRP342
|
4.0
|
25.8
|
1.0
|
CA
|
M:ALA344
|
4.1
|
23.2
|
1.0
|
CA
|
M:SER339
|
4.1
|
27.0
|
1.0
|
N
|
M:ILE341
|
4.1
|
24.2
|
1.0
|
N
|
M:TRP342
|
4.1
|
26.4
|
1.0
|
C
|
M:ASN340
|
4.3
|
25.6
|
1.0
|
C
|
M:TRP342
|
4.3
|
24.7
|
1.0
|
N
|
M:GLY345
|
4.4
|
23.0
|
1.0
|
N
|
M:ASN340
|
4.4
|
26.1
|
1.0
|
CA
|
M:ILE341
|
4.5
|
25.6
|
1.0
|
CB
|
M:ALA344
|
4.5
|
24.3
|
1.0
|
O5'
|
O:DG5
|
4.5
|
23.2
|
1.0
|
O3'
|
O:DC4
|
4.6
|
22.7
|
1.0
|
CB
|
M:SER339
|
4.6
|
24.4
|
1.0
|
CA
|
M:ASN340
|
4.6
|
25.3
|
1.0
|
C
|
M:GLY343
|
4.6
|
24.6
|
1.0
|
CA
|
M:GLY345
|
4.7
|
22.5
|
1.0
|
O
|
M:ASN340
|
4.8
|
27.1
|
1.0
|
CA
|
M:GLY343
|
4.9
|
22.3
|
1.0
|
|
Reference:
M.Garcia-Diaz,
K.Bebenek,
J.M.Krahn,
T.A.Kunkel,
L.C.Pedersen.
A Closed Conformation For the Pol Lambda Catalytic Cycle. Nat.Struct.Mol.Biol. V. 12 97 2005.
ISSN: ISSN 1545-9993
PubMed: 15608652
DOI: 10.1038/NSMB876
Page generated: Mon Oct 7 00:34:21 2024
|