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Sodium in PDB 1xsl: Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap

Enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap

All present enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap:
2.7.7.7;

Protein crystallography data

The structure of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap, PDB code: 1xsl was solved by M.Garcia-Diaz, K.Bebenek, J.M.Krahn, T.A.Kunkel, L.C.Pedersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 28.47 / 2.30
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 191.504, 98.812, 104.519, 90.00, 90.00, 90.00
R / Rfree (%) 20.7 / 25.2

Other elements in 1xsl:

The structure of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap also contains other interesting chemical elements:

Magnesium (Mg) 3 atoms
Arsenic (As) 1 atom

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap (pdb code 1xsl). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 6 binding sites of Sodium where determined in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap, PDB code: 1xsl:
Jump to Sodium binding site number: 1; 2; 3; 4; 5; 6;

Sodium binding site 1 out of 6 in 1xsl

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Sodium binding site 1 out of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Na965

b:52.8
occ:1.00
O J:HOH642 2.2 36.7 1.0
O L:HOH645 2.3 46.0 1.0
O J:HOH641 2.4 57.5 1.0
O J:HOH643 2.4 59.8 1.0
O J:HOH644 2.6 45.3 1.0
O L:HOH640 2.6 52.5 1.0
N7 J:DG2 4.1 50.3 1.0
O J:HOH428 4.2 38.3 1.0
O6 J:DG3 4.4 39.8 1.0
O6 J:DG2 4.4 47.3 1.0
N4 L:DC2 4.9 39.7 1.0
C5 J:DG2 4.9 48.9 1.0
O6 L:DG1 5.0 46.7 1.0

Sodium binding site 2 out of 6 in 1xsl

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Sodium binding site 2 out of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Na966

b:53.3
occ:1.00
O N:HOH943 2.0 36.6 1.0
O N:HOH945 2.2 43.7 1.0
O P:HOH940 2.3 53.8 1.0
O N:HOH944 2.4 56.2 1.0
O6 N:DG3 4.1 29.1 1.0
O6 P:DG1 4.2 33.8 1.0
O N:HOH379 4.3 37.5 1.0
N4 P:DC2 4.4 29.9 1.0
O6 N:DG2 4.5 38.4 1.0
N7 N:DG2 4.5 38.0 1.0

Sodium binding site 3 out of 6 in 1xsl

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Sodium binding site 3 out of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na961

b:30.8
occ:1.00
OP1 C:DG5 2.5 24.3 1.0
O A:SER339 2.5 30.4 1.0
O C:HOH8 2.6 30.5 1.0
O A:ALA344 2.6 26.0 1.0
O A:ILE341 2.7 28.2 1.0
O C:HOH156 2.8 29.3 1.0
O A:HOH1014 3.4 43.3 1.0
C A:SER339 3.4 30.5 1.0
P C:DG5 3.5 25.1 1.0
C A:ILE341 3.6 30.2 1.0
C A:ALA344 3.6 25.7 1.0
OP2 C:DG5 3.7 23.8 1.0
N A:ALA344 3.9 26.2 1.0
CA A:SER339 4.0 31.7 1.0
N A:GLY343 4.1 27.4 1.0
N A:ILE341 4.2 30.8 1.0
O C:HOH905 4.2 62.9 1.0
CA A:TRP342 4.2 29.8 1.0
N A:TRP342 4.3 30.6 1.0
CA A:ALA344 4.3 26.3 1.0
C A:ASN340 4.4 31.9 1.0
CB A:SER339 4.4 29.4 1.0
N A:ASN340 4.5 31.1 1.0
O3' C:DC4 4.5 23.4 1.0
CA A:ILE341 4.5 30.4 1.0
N A:GLY345 4.6 25.7 1.0
C A:TRP342 4.6 29.8 1.0
O5' C:DG5 4.7 24.8 1.0
CA A:ASN340 4.7 31.4 1.0
CA A:GLY345 4.7 27.5 1.0
CB A:ALA344 4.8 23.6 1.0
C A:GLY343 4.9 26.3 1.0
O C:HOH53 4.9 23.9 1.0
O A:ASN340 4.9 33.8 1.0

Sodium binding site 4 out of 6 in 1xsl

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Sodium binding site 4 out of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 4 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Na962

b:55.3
occ:1.00
OP1 G:DG5 2.6 39.0 1.0
O E:SER339 2.6 47.7 1.0
O E:ILE341 2.7 44.6 1.0
O E:ALA344 2.8 40.4 1.0
O E:HOH1016 2.8 51.6 1.0
O G:HOH508 2.9 42.6 1.0
C E:ILE341 3.4 45.4 1.0
C E:SER339 3.4 48.1 1.0
P G:DG5 3.8 41.8 1.0
C E:ALA344 3.8 40.7 1.0
N E:ILE341 3.9 45.9 1.0
N E:TRP342 3.9 43.6 1.0
CA E:TRP342 3.9 43.6 1.0
N E:ALA344 3.9 40.7 1.0
C E:ASN340 4.0 48.0 1.0
N E:GLY343 4.1 41.3 1.0
OP2 G:DG5 4.1 38.2 1.0
CA E:SER339 4.1 49.1 1.0
N E:ASN340 4.2 47.9 1.0
CA E:ILE341 4.3 45.0 1.0
CA E:ALA344 4.3 41.4 1.0
C E:TRP342 4.4 43.6 1.0
CA E:ASN340 4.4 47.9 1.0
O E:ASN340 4.5 49.5 1.0
O5' G:DG5 4.6 39.8 1.0
CB E:ALA344 4.7 40.8 1.0
CB E:SER339 4.7 47.5 1.0
N E:GLY345 4.8 40.0 1.0
C E:GLY343 4.9 41.9 1.0
O3' G:DC4 5.0 41.2 1.0

Sodium binding site 5 out of 6 in 1xsl

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Sodium binding site 5 out of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 5 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Na963

b:53.3
occ:1.00
O K:HOH303 2.5 39.4 1.0
O I:SER339 2.5 34.4 1.0
OP1 K:DG5 2.5 36.1 1.0
O K:HOH875 2.7 49.8 1.0
O I:ALA344 2.8 33.8 1.0
O I:ILE341 2.8 39.9 1.0
C I:ILE341 3.6 38.7 1.0
P K:DG5 3.6 33.6 1.0
C I:SER339 3.6 35.6 1.0
OP2 K:DG5 3.8 35.3 1.0
C I:ALA344 3.8 34.9 1.0
CA I:TRP342 4.0 37.8 1.0
N I:ALA344 4.1 33.8 1.0
N I:TRP342 4.1 38.3 1.0
N I:GLY343 4.1 34.0 1.0
N I:ILE341 4.2 36.7 1.0
CA I:SER339 4.3 36.6 1.0
C I:ASN340 4.4 36.4 1.0
O5' K:DG5 4.4 35.1 1.0
C I:TRP342 4.5 36.4 1.0
CA I:ALA344 4.5 34.9 1.0
CA I:ILE341 4.5 37.6 1.0
N I:ASN340 4.6 34.9 1.0
CA I:ASN340 4.7 36.0 1.0
O I:ASN340 4.7 37.5 1.0
N I:GLY345 4.8 35.0 1.0
CB I:SER339 4.8 36.6 1.0
O3' K:DC4 4.9 36.5 1.0
CA I:GLY345 4.9 35.2 1.0
CB I:ALA344 5.0 36.0 1.0

Sodium binding site 6 out of 6 in 1xsl

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Sodium binding site 6 out of 6 in the Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 6 of Crystal Structure of Human Dna Polymerase Lambda in Complex with A One Nucleotide Dna Gap within 5.0Å range:
probe atom residue distance (Å) B Occ
M:Na964

b:29.6
occ:1.00
OP1 O:DG5 2.3 20.6 1.0
O M:SER339 2.4 26.9 1.0
O M:ALA344 2.4 20.4 1.0
O M:ILE341 2.6 27.8 1.0
O O:HOH39 2.6 21.9 1.0
O M:HOH978 2.7 26.6 1.0
C M:ALA344 3.4 22.5 1.0
C M:SER339 3.4 25.8 1.0
O M:HOH1071 3.5 36.6 1.0
C M:ILE341 3.5 26.4 1.0
P O:DG5 3.5 22.6 1.0
N M:ALA344 3.6 22.7 1.0
OP2 O:DG5 3.8 23.5 1.0
N M:GLY343 3.9 24.4 1.0
CA M:TRP342 4.0 25.8 1.0
CA M:ALA344 4.1 23.2 1.0
CA M:SER339 4.1 27.0 1.0
N M:ILE341 4.1 24.2 1.0
N M:TRP342 4.1 26.4 1.0
C M:ASN340 4.3 25.6 1.0
C M:TRP342 4.3 24.7 1.0
N M:GLY345 4.4 23.0 1.0
N M:ASN340 4.4 26.1 1.0
CA M:ILE341 4.5 25.6 1.0
CB M:ALA344 4.5 24.3 1.0
O5' O:DG5 4.5 23.2 1.0
O3' O:DC4 4.6 22.7 1.0
CB M:SER339 4.6 24.4 1.0
CA M:ASN340 4.6 25.3 1.0
C M:GLY343 4.6 24.6 1.0
CA M:GLY345 4.7 22.5 1.0
O M:ASN340 4.8 27.1 1.0
CA M:GLY343 4.9 22.3 1.0

Reference:

M.Garcia-Diaz, K.Bebenek, J.M.Krahn, T.A.Kunkel, L.C.Pedersen. A Closed Conformation For the Pol Lambda Catalytic Cycle. Nat.Struct.Mol.Biol. V. 12 97 2005.
ISSN: ISSN 1545-9993
PubMed: 15608652
DOI: 10.1038/NSMB876
Page generated: Mon Oct 7 00:34:21 2024

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