Sodium in PDB 1xfg: Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate

All present enzymatic activity of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate:
2.6.1.16;

The structure of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate, PDB code: 1xfg was solved by M.N.Isupov, A.Teplyakov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 1.85
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.250, 82.400, 85.830, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / n/a

The binding sites of Sodium atom in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate (pdb code 1xfg). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate, PDB code: 1xfg:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate within 5.0Å range: probe atom residue distance (Å) B Occ A:Na5301 b:34.1 occ:1.00 O A:GLY154 2.2 21.0 1.0 O A:HOH5397 2.3 41.5 1.0 OG A:SER174 2.4 23.8 1.0 O A:SER174 2.5 18.3 1.0 O A:HOH5356 2.8 31.4 1.0 C A:SER174 3.3 19.1 1.0 CB A:SER174 3.3 24.4 1.0 C A:GLY154 3.4 17.7 1.0 CA A:SER174 3.8 18.8 1.0 CE2 A:TYR156 3.8 18.6 1.0 CD2 A:TYR156 4.0 20.5 1.0 CA A:ALA155 4.1 18.7 1.0 N A:ALA155 4.2 20.2 1.0 N A:GLY154 4.2 17.1 1.0 OE2 A:GLU208 4.2 24.9 1.0 O A:HOH5348 4.3 29.6 1.0 N A:GLY175 4.4 16.0 1.0 CA A:GLY154 4.4 20.3 1.0 N A:TYR156 4.5 17.4 1.0 CZ A:TYR156 4.6 25.4 1.0 C A:ALA155 4.8 22.5 1.0 CA A:GLY175 4.8 21.4 1.0 O A:HOH5439 5.0 52.5 1.0

Sodium binding site 2 out of 2 in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with L-Glu Hydroxamate within 5.0Å range: probe atom residue distance (Å) B Occ B:Na9301 b:42.2 occ:1.00 O B:HOH9359 2.1 35.7 1.0 O B:GLY154 2.2 28.9 1.0 OG B:SER174 2.4 33.7 1.0 O B:SER174 2.6 21.6 1.0 O B:HOH9385 2.8 42.7 1.0 C B:SER174 3.4 22.9 1.0 C B:GLY154 3.4 27.6 1.0 CB B:SER174 3.4 30.9 1.0 CE2 B:TYR156 3.7 22.2 1.0 CA B:SER174 3.9 20.8 1.0 CD2 B:TYR156 4.0 23.3 1.0 O B:HOH9421 4.0 50.6 1.0 N B:GLY154 4.1 21.0 1.0 CA B:ALA155 4.2 23.1 1.0 O B:HOH9367 4.2 38.8 1.0 N B:ALA155 4.3 24.7 1.0 CA B:GLY154 4.4 30.7 1.0 N B:GLY175 4.4 19.5 1.0 OE2 B:GLU208 4.5 33.1 1.0 CZ B:TYR156 4.5 34.3 1.0 N B:TYR156 4.6 26.9 1.0 O B:HOH9379 4.7 41.3 1.0 OH B:TYR156 4.8 29.0 1.0 CA B:GLY175 4.9 29.7 1.0 O B:HOH9400 4.9 46.2 1.0 C B:ALA155 4.9 26.1 1.0

M.N.Isupov, G.Obmolova, S.Butterworth, M.-A.Badet-Denisot, B.Badet, I.Polikarpov, J.A.Littlechild, A.Teplyakov. Substrate Binding Is Required For Assembly of the Active Conformation of the Catalytic Site in Ntn Amidotransferases: Evidence From the 1.8 Angstrom Crystal Structure of the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Structure V. 4 801 1996.
ISSN: ISSN 0969-2126
PubMed: 8805567
DOI: 10.1016/S0969-2126(96)00087-1