Sodium in PDB 1xff: Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate

All present enzymatic activity of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate:
2.6.1.16;

The structure of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate, PDB code: 1xff was solved by M.N.Isupov, A.Teplyakov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.380, 82.520, 86.050, 90.00, 90.00, 90.00
R / Rfree (%)	16.1 / n/a

The binding sites of Sodium atom in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate (pdb code 1xff). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate, PDB code: 1xff:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate within 5.0Å range: probe atom residue distance (Å) B Occ A:Na5301 b:30.2 occ:1.00 O A:GLY154 2.1 20.8 1.0 O A:HOH5363 2.3 32.1 1.0 OG A:SER174 2.4 21.6 1.0 O A:SER174 2.5 17.0 1.0 O A:HOH5344 2.7 27.8 1.0 C A:SER174 3.3 16.1 1.0 C A:GLY154 3.3 15.1 1.0 CB A:SER174 3.4 22.2 1.0 CA A:SER174 3.8 14.8 1.0 CE2 A:TYR156 3.9 17.9 1.0 CA A:ALA155 4.1 17.9 1.0 CD2 A:TYR156 4.1 16.7 1.0 N A:GLY154 4.1 17.6 1.0 N A:ALA155 4.2 16.4 1.0 O A:HOH5357 4.2 30.6 1.0 OE2 A:GLU208 4.3 22.5 1.0 N A:GLY175 4.3 14.4 1.0 CA A:GLY154 4.4 16.2 1.0 N A:TYR156 4.5 16.6 1.0 CZ A:TYR156 4.7 26.2 1.0 CA A:GLY175 4.7 18.6 1.0 O A:HOH5429 4.8 46.8 1.0 C A:ALA155 4.9 20.8 1.0

Sodium binding site 2 out of 2 in the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Glutaminase Domain of Glucosamine 6-Phosphate Synthase Complexed with Glutamate within 5.0Å range: probe atom residue distance (Å) B Occ B:Na9301 b:38.9 occ:1.00 O B:HOH9348 2.1 32.5 1.0 O B:GLY154 2.2 28.9 1.0 OG B:SER174 2.5 30.5 1.0 O B:SER174 2.7 21.4 1.0 O B:HOH9379 2.9 41.3 1.0 C B:GLY154 3.4 21.7 1.0 CB B:SER174 3.5 28.0 1.0 C B:SER174 3.5 21.4 1.0 CE2 B:TYR156 3.6 21.3 1.0 CD2 B:TYR156 4.0 22.2 1.0 O B:HOH9426 4.0 52.9 1.0 CA B:SER174 4.0 20.5 1.0 N B:GLY154 4.0 20.9 1.0 O B:HOH9371 4.1 39.1 1.0 CA B:ALA155 4.2 23.2 1.0 N B:ALA155 4.2 22.5 1.0 CA B:GLY154 4.3 27.4 1.0 CZ B:TYR156 4.5 32.3 1.0 N B:GLY175 4.5 18.9 1.0 N B:TYR156 4.6 18.9 1.0 OE2 B:GLU208 4.6 30.1 1.0 O B:HOH9389 4.7 43.0 1.0 OH B:TYR156 4.8 31.1 1.0 C B:ALA155 5.0 28.2 1.0 C B:ARG153 5.0 28.7 1.0 O B:HOH9381 5.0 41.8 1.0 CG B:TYR156 5.0 19.1 1.0 CA B:GLY175 5.0 26.2 1.0

M.N.Isupov, G.Obmolova, S.Butterworth, M.-A.Badet-Denisot, B.Badet, I.Polikarpov, J.A.Littlechild, A.Teplyakov. Substrate Binding Is Required For Assembly of the Active Conformation of the Catalytic Site in Ntn Amidotransferases: Evidence From the 1.8 Angstrom Crystal Structure of the Glutaminase Domain of Glucosamine 6-Phosphate Synthase Structure V. 4 801 1996.
ISSN: ISSN 0969-2126
PubMed: 8805567
DOI: 10.1016/S0969-2126(96)00087-1