Atomistry » Sodium » PDB 3r9b-3rn1 » 3rjk
Atomistry »
  Sodium »
    PDB 3r9b-3rn1 »
      3rjk »

Sodium in PDB 3rjk: Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site

Enzymatic activity of Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site

All present enzymatic activity of Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site:
2.7.7.7;

Protein crystallography data

The structure of Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site, PDB code: 3rjk was solved by V.K.Batra, W.A.Beard, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 22.32 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 50.650, 79.970, 55.510, 90.00, 107.75, 90.00
R / Rfree (%) 20 / 25.8

Other elements in 3rjk:

The structure of Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site also contains other interesting chemical elements:

Fluorine (F) 2 atoms
Magnesium (Mg) 2 atoms
Chlorine (Cl) 3 atoms

Sodium Binding Sites:

The binding sites of Sodium atom in the Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site (pdb code 3rjk). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site, PDB code: 3rjk:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 3rjk

Go back to Sodium Binding Sites List in 3rjk
Sodium binding site 1 out of 2 in the Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na341

b:21.5
occ:1.00
O A:HOH644 2.3 37.3 1.0
OP1 P:DG9 2.3 15.0 1.0
O A:THR101 2.4 16.4 1.0
O A:VAL103 2.5 13.4 1.0
O A:HOH390 2.6 20.1 1.0
O A:ILE106 2.6 12.0 1.0
C A:THR101 3.4 17.0 1.0
C A:VAL103 3.4 14.0 1.0
P P:DG9 3.5 15.9 1.0
C A:ILE106 3.6 13.2 1.0
N A:ILE106 3.7 13.2 1.0
OP2 P:DG9 3.7 17.7 1.0
N A:GLY105 3.8 13.6 1.0
CA A:SER104 3.8 14.5 1.0
N A:SER104 4.0 14.9 1.0
N A:VAL103 4.1 14.7 1.0
CA A:THR101 4.1 17.4 1.0
CA A:ILE106 4.2 12.2 1.0
C A:SER104 4.2 13.2 1.0
C A:ARG102 4.3 13.6 1.0
N A:ARG102 4.3 14.4 1.0
O3' P:DC8 4.4 15.2 1.0
CA A:VAL103 4.4 14.6 1.0
N A:GLY107 4.5 13.1 1.0
CA A:ARG102 4.5 13.6 1.0
CB A:ILE106 4.6 13.1 1.0
C A:GLY105 4.6 13.1 1.0
O5' P:DG9 4.7 17.5 1.0
CB A:THR101 4.7 17.8 1.0
O A:ARG102 4.7 14.8 1.0
CA A:GLY107 4.8 13.6 1.0
CA A:GLY105 4.8 14.5 1.0
O A:LEU100 4.9 16.8 1.0

Sodium binding site 2 out of 2 in 3rjk

Go back to Sodium Binding Sites List in 3rjk
Sodium binding site 2 out of 2 in the Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Ternary Complex of Dna Polymerase Beta with A Gapped Dna Containing 8ODG:Dc Base Pair at Primer Terminus and Dg:Dcmp(CF2)Pp in the Active Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na342

b:31.8
occ:1.00
O A:VAL65 2.2 23.8 1.0
O A:HOH672 2.4 27.7 1.0
O A:LYS60 2.5 24.5 1.0
OP1 D:DC3 2.5 26.3 1.0
O A:LEU62 2.6 23.8 1.0
O A:HOH573 2.9 33.3 1.0
C A:VAL65 3.4 22.1 1.0
C A:LYS60 3.5 26.3 1.0
P D:DC3 3.5 24.8 1.0
OP2 D:DC3 3.7 24.1 1.0
C A:LEU62 3.7 25.1 1.0
N A:VAL65 3.9 20.8 1.0
CA A:VAL65 4.1 20.1 1.0
CA A:LYS60 4.1 25.1 1.0
CG A:LYS60 4.2 26.9 1.0
CA A:PRO63 4.3 25.3 1.0
N A:GLY64 4.3 23.2 1.0
C A:LYS61 4.3 26.6 1.0
CB A:VAL65 4.4 20.0 1.0
N A:LEU62 4.4 25.7 1.0
N A:PRO63 4.4 25.2 1.0
N A:GLY66 4.4 22.4 1.0
N A:LYS61 4.4 26.7 1.0
O A:LYS61 4.5 26.2 1.0
O3' D:DT2 4.5 25.1 1.0
CA A:GLY66 4.6 22.6 1.0
CA A:LYS61 4.7 27.1 1.0
CA A:LEU62 4.7 25.5 1.0
C A:PRO63 4.7 23.8 1.0
O5' D:DC3 4.8 28.0 1.0
NZ A:LYS60 4.8 30.8 1.0
CB A:LYS60 4.8 25.9 1.0
C A:GLY64 4.9 21.7 1.0
O A:ALA59 5.0 22.3 1.0

Reference:

V.K.Batra, D.D.Shock, W.A.Beard, C.E.Mckenna, S.H.Wilson. Binary Complex Crystal Structure of Dna Polymerase Beta Reveals Multiple Conformations of the Templating 8-Oxoguanine Lesion. Proc.Natl.Acad.Sci.Usa V. 109 113 2012.
ISSN: ISSN 0027-8424
PubMed: 22178760
DOI: 10.1073/PNAS.1112235108
Page generated: Mon Oct 7 12:49:16 2024

Last articles

Cl in 5GS8
Cl in 5GQQ
Cl in 5G6U
Cl in 5GQN
Cl in 5GQM
Cl in 5GQL
Cl in 5GQK
Cl in 5GQJ
Cl in 5GNK
Cl in 5GQI
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy