Atomistry » Sodium » PDB 3h0n-3hwd » 3hw8
Atomistry »
  Sodium »
    PDB 3h0n-3hwd »
      3hw8 »

Sodium in PDB 3hw8: Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site

Enzymatic activity of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site

All present enzymatic activity of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site:
2.7.7.7;

Protein crystallography data

The structure of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site, PDB code: 3hw8 was solved by M.Garcia-Diaz, K.Bebenek, A.A.Larrea, J.M.Havener, L.Perera, J.M.Krahn, L.C.Pedersen, D.A.Ramsden, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.32 / 1.95
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 56.249, 68.386, 137.108, 90.00, 90.00, 90.00
R / Rfree (%) 21.2 / 23.3

Other elements in 3hw8:

The structure of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Sodium Binding Sites:

The binding sites of Sodium atom in the Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site (pdb code 3hw8). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 4 binding sites of Sodium where determined in the Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site, PDB code: 3hw8:
Jump to Sodium binding site number: 1; 2; 3; 4;

Sodium binding site 1 out of 4 in 3hw8

Go back to Sodium Binding Sites List in 3hw8
Sodium binding site 1 out of 4 in the Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na577

b:25.9
occ:1.00
OD1 A:ASP429 2.1 25.0 1.0
OD1 A:ASP427 2.2 30.1 1.0
O1A A:D3T581 2.5 19.3 1.0
O C:HOH17 2.5 22.2 1.0
OD2 A:ASP490 2.7 36.5 1.0
CG A:ASP427 3.1 28.1 1.0
CG A:ASP429 3.2 25.3 1.0
OD2 A:ASP427 3.4 24.8 1.0
CB A:ASP490 3.4 27.0 1.0
PA A:D3T581 3.5 23.1 1.0
CG A:ASP490 3.5 33.6 1.0
O5' A:D3T581 3.6 23.6 1.0
MG A:MG576 3.6 24.5 1.0
OD2 A:ASP429 3.6 24.7 1.0
C5' A:D3T581 3.9 23.1 1.0
O A:HOH93 3.9 33.4 1.0
O2A A:D3T581 4.1 25.9 1.0
C3' C:DT6 4.1 20.6 1.0
C5' C:DT6 4.5 21.6 1.0
CB A:ASP429 4.5 22.3 1.0
C4' C:DT6 4.5 21.8 1.0
CB A:ASP427 4.5 26.3 1.0
OD1 A:ASP490 4.6 36.0 1.0
O3G A:D3T581 4.8 21.6 1.0
O3A A:D3T581 4.8 21.5 1.0
OP1 C:DT6 4.8 21.4 1.0
CA A:ASP490 4.9 25.2 1.0

Sodium binding site 2 out of 4 in 3hw8

Go back to Sodium Binding Sites List in 3hw8
Sodium binding site 2 out of 4 in the Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na578

b:22.2
occ:1.00
O A:ILE341 2.4 17.8 1.0
OP1 C:DA5 2.4 16.9 1.0
O A:HOH14 2.4 23.3 1.0
O A:SER339 2.4 21.2 1.0
O A:ALA344 2.5 20.9 1.0
O C:HOH40 2.5 29.6 1.0
C A:ILE341 3.4 19.9 1.0
O C:HOH82 3.4 39.9 1.0
C A:SER339 3.4 22.1 1.0
P C:DA5 3.5 19.9 1.0
C A:ALA344 3.5 19.8 1.0
N A:ALA344 3.7 17.2 1.0
OP2 C:DA5 3.7 17.4 1.0
O A:HOH181 3.8 46.8 1.0
N A:GLY343 3.9 18.0 1.0
CA A:TRP342 3.9 20.2 1.0
N A:ILE341 3.9 19.0 1.0
N A:TRP342 4.0 19.2 1.0
C A:ASN340 4.2 19.9 1.0
CA A:ALA344 4.2 19.1 1.0
C A:TRP342 4.3 19.6 1.0
CA A:SER339 4.3 22.0 1.0
N A:ASN340 4.3 21.2 1.0
O3' C:DT4 4.3 17.7 1.0
CA A:ILE341 4.4 18.9 1.0
CA A:ASN340 4.4 23.5 1.0
N A:GLY345 4.6 19.1 1.0
C A:GLY343 4.6 18.8 1.0
CB A:ALA344 4.7 17.8 1.0
O A:ASN340 4.7 22.4 1.0
O5' C:DA5 4.8 19.7 1.0
CA A:GLY343 4.8 19.5 1.0
O A:HOH60 4.9 49.2 1.0
CA A:GLY345 4.9 18.6 1.0
O C:HOH179 4.9 54.9 1.0
CB A:SER339 4.9 21.4 1.0

Sodium binding site 3 out of 4 in 3hw8

Go back to Sodium Binding Sites List in 3hw8
Sodium binding site 3 out of 4 in the Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na579

b:39.8
occ:1.00
O B:HOH62 2.4 40.5 1.0
O B:HOH168 2.4 44.7 1.0
O A:SER463 2.4 27.7 1.0
O A:HOH48 2.5 30.1 1.0
O A:HOH136 2.5 49.3 1.0
O B:HOH78 2.7 38.2 1.0
C A:SER463 3.6 28.2 1.0
O B:HOH216 3.6 50.1 1.0
OP1 B:DT11 4.0 23.7 1.0
N A:GLU465 4.3 33.0 1.0
OE2 A:GLU466 4.3 58.1 1.0
N A:GLN464 4.4 28.2 1.0
CA A:GLN464 4.4 31.1 1.0
CA A:SER463 4.5 27.7 1.0
OE1 A:GLU466 4.5 60.1 1.0
OP2 B:DT11 4.6 27.8 1.0
CB A:SER463 4.6 28.1 1.0
N A:SER463 4.7 26.0 1.0
P B:DT11 4.8 25.4 1.0
OE2 A:GLU465 4.8 38.0 1.0
CD A:GLU466 4.9 56.9 1.0
C A:GLN464 4.9 32.5 1.0

Sodium binding site 4 out of 4 in 3hw8

Go back to Sodium Binding Sites List in 3hw8
Sodium binding site 4 out of 4 in the Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 4 of Ternary Complex of Dna Polymerase Lambda of A Two Nucleotide Gapped Dna Substrate with A C in the Scrunch Site within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na580

b:51.2
occ:1.00
O A:ILE302 2.3 52.6 1.0
O A:HOH648 2.3 47.7 1.0
O A:CYS300 2.4 59.2 1.0
O A:ILE305 2.5 53.5 1.0
O A:HOH90 2.6 38.0 1.0
C A:ILE302 3.0 52.9 1.0
OP1 D:DC3 3.4 40.9 1.0
C A:CYS300 3.4 59.1 1.0
C A:ILE305 3.6 53.0 1.0
N A:PRO303 3.6 51.8 1.0
N A:ILE302 3.7 55.0 1.0
CA A:PRO303 3.7 51.0 1.0
OP2 D:DC3 3.7 41.0 1.0
N A:ILE305 3.8 49.6 1.0
CA A:ILE302 3.9 54.0 1.0
C A:SER301 4.0 55.7 1.0
P D:DC3 4.0 42.8 1.0
N A:GLY304 4.1 48.7 1.0
CA A:ILE305 4.1 51.8 1.0
CA A:CYS300 4.2 60.8 1.0
C A:PRO303 4.3 50.0 1.0
N A:SER301 4.3 58.0 1.0
O A:ALA299 4.3 63.4 1.0
O A:SER301 4.4 54.8 1.0
CB A:ILE305 4.5 51.8 1.0
CA A:SER301 4.5 56.4 1.0
N A:GLY306 4.6 54.0 1.0
CD A:PRO303 4.9 52.1 1.0
CA A:GLY306 4.9 56.3 1.0
CB A:ILE302 4.9 54.3 1.0
C A:GLY304 4.9 48.9 1.0
CB A:PRO303 5.0 51.5 1.0

Reference:

M.Garcia-Diaz, K.Bebenek, A.A.Larrea, J.M.Havener, L.Perera, J.M.Krahn, L.C.Pedersen, D.A.Ramsden, T.A.Kunkel. Scrunching Durning Dna Repair Synthesis To Be Published.
Page generated: Sun Aug 17 15:22:42 2025

Last articles

Mn in 9LJU
Mn in 9LJW
Mn in 9LJS
Mn in 9LJR
Mn in 9LJT
Mn in 9LJV
Mg in 9UA2
Mg in 9R96
Mg in 9VM1
Mg in 9P01
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy