Sodium in PDB 1z45: Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose

Enzymatic activity of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose

All present enzymatic activity of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose:
5.1.3.2; 5.1.3.3;

Protein crystallography data

The structure of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose, PDB code: 1z45 was solved by J.B.Thoden, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	30.00 / 1.85
*Space group*	I 2 2 2
*Cell size a, b, c (Å), α, β, γ (°)*	120.900, 125.200, 142.300, 90.00, 90.00, 90.00
*R / R_free (%)*	n/a / n/a

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose (pdb code 1z45). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose, PDB code: 1z45:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 1z45

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Sodium Binding Sites List in 1z45

Sodium binding site 1 out of 2 in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose

Mono view

Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Na701 b:38.0 occ:1.00	O	A:LEU357	2.3	16.7	1.0
	O	A:VAL360	2.4	17.8	1.0
	O	A:HOH1054	2.5	38.7	1.0
	O	A:HOH1053	2.5	32.9	1.0
	O	A:HOH1223	2.5	47.7	1.0
	O	A:HOH1055	2.5	43.7	1.0
	C	A:LEU357	3.4	17.0	1.0
	C	A:VAL360	3.5	32.0	1.0
	N	A:LEU357	3.9	21.4	1.0
	N	A:VAL360	4.0	16.4	1.0
	CA	A:VAL360	4.1	19.3	1.0
	CB	A:VAL360	4.2	19.8	1.0
	CA	A:LEU357	4.2	19.8	1.0
	OE2	A:GLU361	4.2	53.2	1.0
	OE1	A:GLN356	4.3	62.7	1.0
	N	A:ARG358	4.3	15.2	1.0
	CA	A:ARG358	4.4	18.7	1.0
	O	A:HOH1405	4.5	52.3	1.0
	CD	A:GLU361	4.5	0.0	1.0
	O	A:HOH1276	4.5	56.9	1.0
	N	A:GLU361	4.6	19.2	1.0
	OE1	A:GLU361	4.6	59.8	1.0
	N	A:GLY359	4.7	23.3	1.0
	CG1	A:VAL360	4.7	15.6	1.0
	C	A:ARG358	4.8	28.6	1.0
	O	A:HOH882	4.8	33.3	1.0
	CA	A:GLU361	4.8	17.0	1.0
	C	A:GLN356	4.9	22.2	1.0
	CB	A:LEU357	4.9	19.7	1.0

Sodium binding site 2 out of 2 in 1z45

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Sodium Binding Sites List in 1z45

Sodium binding site 2 out of 2 in the Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose

Mono view

Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of the GAL10 Fusion Protein Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae Complexed with Nad, Udp-Glucose, and Galactose within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Na702 b:51.5 occ:1.00	OE1	A:GLN475	2.3	23.1	1.0
	O	A:HOH817	2.3	16.7	1.0
	O	A:GLU350	2.4	23.7	1.0
	O	A:HOH816	2.4	26.5	1.0
	OD1	A:ASN351	2.8	20.3	1.0
	O	A:HOH1106	3.0	31.9	1.0
	C	A:GLU350	3.4	25.7	1.0
	O	A:HOH819	3.4	22.6	1.0
	CD	A:GLN475	3.4	25.3	1.0
	CG	A:ASN351	3.5	21.5	1.0
	O	A:HOH815	3.8	45.5	1.0
	CB	A:GLN475	3.9	12.3	1.0
	CA	A:ASN351	4.0	22.2	1.0
	N	A:ASN351	4.1	21.1	1.0
	CB	A:ASN351	4.2	14.2	1.0
	ND2	A:ASN351	4.2	13.7	1.0
	O	A:HOH786	4.2	23.7	1.0
	CG	A:GLN475	4.3	28.1	1.0
	NE2	A:GLN475	4.3	20.2	1.0
	CA	A:GLU350	4.3	15.9	1.0
	CA	A:GLN475	4.4	10.2	1.0
	O	A:HOH822	4.4	29.9	1.0
	N	A:ASN476	4.5	22.0	1.0
	NH2	A:ARG54	4.5	20.7	1.0
	CB	A:GLU350	4.5	17.0	1.0
	O	A:HOH814	4.6	32.8	1.0
	O	A:HOH1105	4.7	29.6	1.0
	O	A:HOH821	4.9	17.9	1.0
	OE1	A:GLU350	5.0	41.3	1.0

Reference:

J.B.Thoden, H.M.Holden. The Molecular Architecture of Galactose Mutarotase/Udp-Galactose 4-Epimerase From Saccharomyces Cerevisiae. J.Biol.Chem. V. 280 21900 2005.
ISSN: ISSN 0021-9258
PubMed: 15795221
DOI: 10.1074/JBC.M502411200

Page generated: Mon Oct 7 01:40:50 2024

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