Sodium in PDB 8pej: CJGH35 with A Galactosidase Activity-Based Probe
Enzymatic activity of CJGH35 with A Galactosidase Activity-Based Probe
All present enzymatic activity of CJGH35 with A Galactosidase Activity-Based Probe:
3.2.1.23;
Protein crystallography data
The structure of CJGH35 with A Galactosidase Activity-Based Probe, PDB code: 8pej
was solved by
W.A.Offen,
G.J.Davies,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
82.54 /
1.50
|
Space group
|
P 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
99.316,
116.212,
116.396,
90.24,
89.89,
90.09
|
R / Rfree (%)
|
15.7 /
18.1
|
Sodium Binding Sites:
Sodium binding site 1 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 1 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na601
b:29.8
occ:1.00
|
O
|
A:GLY466
|
2.3
|
25.6
|
1.0
|
O
|
A:HOH1097
|
2.3
|
42.9
|
1.0
|
OD1
|
A:ASP464
|
2.5
|
30.1
|
1.0
|
OG
|
A:SER518
|
2.5
|
31.0
|
1.0
|
OE1
|
A:GLN519
|
2.5
|
36.6
|
1.0
|
O
|
A:HOH744
|
2.6
|
35.6
|
1.0
|
HG
|
A:SER518
|
2.9
|
30.8
|
0.0
|
HB3
|
A:SER518
|
3.1
|
29.8
|
1.0
|
CG
|
A:ASP464
|
3.3
|
31.4
|
1.0
|
CB
|
A:SER518
|
3.3
|
29.8
|
1.0
|
C
|
A:GLY466
|
3.3
|
23.6
|
1.0
|
HE22
|
A:GLN519
|
3.4
|
40.7
|
1.0
|
NA
|
A:NA605
|
3.4
|
34.3
|
1.0
|
H
|
A:GLY466
|
3.4
|
24.9
|
1.0
|
OD2
|
A:ASP464
|
3.4
|
37.8
|
1.0
|
HA2
|
A:GLY466
|
3.5
|
25.0
|
1.0
|
CD
|
A:GLN519
|
3.5
|
44.2
|
1.0
|
HB2
|
A:SER518
|
3.7
|
29.8
|
1.0
|
CA
|
A:GLY466
|
3.7
|
25.7
|
1.0
|
NE2
|
A:GLN519
|
3.8
|
39.4
|
1.0
|
N
|
A:GLY466
|
3.9
|
24.4
|
1.0
|
OD1
|
A:ASP469
|
4.4
|
27.4
|
1.0
|
O
|
A:HOH1120
|
4.4
|
46.7
|
1.0
|
N
|
A:LEU467
|
4.5
|
25.6
|
1.0
|
HA
|
A:LEU467
|
4.6
|
26.6
|
1.0
|
CB
|
A:ASP464
|
4.6
|
27.1
|
1.0
|
CA
|
A:SER518
|
4.6
|
28.9
|
1.0
|
O
|
A:HOH850
|
4.7
|
35.5
|
1.0
|
HE21
|
A:GLN519
|
4.7
|
40.8
|
1.0
|
HA3
|
A:GLY466
|
4.7
|
25.0
|
1.0
|
HA
|
A:ASP464
|
4.8
|
25.5
|
1.0
|
CG
|
A:GLN519
|
4.9
|
43.7
|
1.0
|
C
|
A:SER518
|
5.0
|
34.0
|
1.0
|
HB2
|
A:ASP464
|
5.0
|
27.5
|
1.0
|
HG3
|
A:GLN519
|
5.0
|
42.7
|
1.0
|
CA
|
A:LEU467
|
5.0
|
26.8
|
1.0
|
|
Sodium binding site 2 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 2 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na604
b:27.3
occ:1.00
|
OE1
|
A:GLU544
|
2.5
|
28.1
|
1.0
|
O
|
A:HOH916
|
2.5
|
23.9
|
1.0
|
OE2
|
A:GLU533
|
2.6
|
22.1
|
1.0
|
O
|
A:HOH837
|
2.6
|
36.2
|
1.0
|
O
|
A:HOH812
|
2.8
|
34.7
|
1.0
|
O
|
A:HOH1140
|
3.1
|
55.0
|
1.0
|
HG2
|
A:GLU533
|
3.1
|
19.7
|
1.0
|
CD
|
A:GLU533
|
3.5
|
21.4
|
1.0
|
CD
|
A:GLU544
|
3.5
|
27.4
|
1.0
|
CG
|
A:GLU533
|
3.6
|
19.7
|
1.0
|
HG3
|
A:GLU533
|
3.7
|
19.7
|
1.0
|
OE2
|
A:GLU544
|
3.8
|
31.0
|
1.0
|
O
|
A:HOH988
|
3.8
|
26.0
|
1.0
|
HA
|
A:HIS563
|
3.8
|
19.9
|
1.0
|
HD1
|
A:HIS563
|
3.9
|
20.3
|
0.0
|
O
|
A:HOH1020
|
4.0
|
36.6
|
1.0
|
O
|
B:HOH798
|
4.1
|
44.6
|
1.0
|
H
|
A:LEU564
|
4.1
|
18.9
|
1.0
|
O
|
A:PRO562
|
4.4
|
21.5
|
1.0
|
O
|
A:GLY534
|
4.4
|
22.4
|
1.0
|
OE1
|
A:GLU533
|
4.6
|
20.6
|
1.0
|
ND1
|
A:HIS563
|
4.7
|
20.2
|
1.0
|
O
|
A:HOH1108
|
4.7
|
28.7
|
1.0
|
O
|
A:HOH1152
|
4.7
|
28.2
|
1.0
|
CA
|
A:HIS563
|
4.8
|
19.8
|
1.0
|
O
|
A:HOH1157
|
4.8
|
30.1
|
1.0
|
H
|
A:GLY534
|
4.8
|
19.9
|
1.0
|
HB2
|
A:GLU544
|
4.8
|
22.4
|
1.0
|
HA2
|
A:GLY534
|
4.9
|
21.4
|
1.0
|
CG
|
A:GLU544
|
4.9
|
24.2
|
1.0
|
N
|
A:GLY534
|
5.0
|
19.6
|
1.0
|
O
|
A:HOH950
|
5.0
|
32.4
|
1.0
|
|
Sodium binding site 3 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 3 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na605
b:34.3
occ:1.00
|
O
|
A:HOH850
|
2.3
|
35.5
|
1.0
|
O
|
A:HOH1102
|
2.3
|
48.5
|
1.0
|
OG
|
A:SER518
|
2.4
|
31.0
|
1.0
|
O
|
A:HOH744
|
2.4
|
35.6
|
1.0
|
O
|
A:SER518
|
2.5
|
33.5
|
1.0
|
OE1
|
A:GLN519
|
2.6
|
36.6
|
1.0
|
HG
|
A:SER518
|
2.8
|
30.8
|
0.0
|
C
|
A:SER518
|
3.2
|
34.0
|
1.0
|
NA
|
A:NA601
|
3.4
|
29.8
|
1.0
|
HG3
|
A:GLN519
|
3.4
|
42.7
|
1.0
|
CB
|
A:SER518
|
3.4
|
29.8
|
1.0
|
CD
|
A:GLN519
|
3.5
|
44.2
|
1.0
|
CA
|
A:SER518
|
3.7
|
28.9
|
1.0
|
HB3
|
A:SER518
|
3.7
|
29.8
|
1.0
|
HA
|
A:SER518
|
3.7
|
29.2
|
1.0
|
CG
|
A:GLN519
|
4.0
|
43.7
|
1.0
|
N
|
A:GLN519
|
4.1
|
36.9
|
1.0
|
HA
|
A:GLN519
|
4.1
|
39.0
|
1.0
|
HB2
|
A:SER518
|
4.2
|
29.8
|
1.0
|
OD1
|
A:ASP469
|
4.3
|
27.4
|
1.0
|
OD2
|
A:ASP469
|
4.3
|
31.2
|
1.0
|
CA
|
A:GLN519
|
4.6
|
39.0
|
1.0
|
OD2
|
A:ASP464
|
4.6
|
37.8
|
1.0
|
NE2
|
A:GLN519
|
4.6
|
39.4
|
1.0
|
OD1
|
A:ASP464
|
4.6
|
30.1
|
1.0
|
HG2
|
A:GLN519
|
4.7
|
42.8
|
1.0
|
CG
|
A:ASP464
|
4.7
|
31.4
|
1.0
|
CG
|
A:ASP469
|
4.7
|
27.1
|
1.0
|
HE22
|
A:GLN519
|
4.8
|
40.7
|
1.0
|
H
|
A:GLN519
|
4.8
|
36.5
|
1.0
|
CB
|
A:GLN519
|
4.8
|
39.4
|
1.0
|
O
|
A:HOH754
|
4.9
|
35.5
|
1.0
|
O
|
A:GLY466
|
5.0
|
25.6
|
1.0
|
|
Sodium binding site 4 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 4 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 4 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na606
b:32.0
occ:1.00
|
O
|
A:HIS49
|
2.5
|
22.8
|
1.0
|
O
|
A:GLY367
|
2.6
|
22.6
|
1.0
|
O
|
A:HOH1031
|
2.9
|
31.0
|
1.0
|
OG
|
A:SER417
|
2.9
|
34.5
|
1.0
|
O
|
A:HOH930
|
3.2
|
27.7
|
1.0
|
HA3
|
A:GLY367
|
3.4
|
22.0
|
1.0
|
HA2
|
A:GLY367
|
3.4
|
22.0
|
1.0
|
C
|
A:GLY367
|
3.5
|
22.2
|
1.0
|
HB3
|
A:SER417
|
3.5
|
27.9
|
1.0
|
HG
|
A:SER417
|
3.6
|
34.1
|
0.0
|
H
|
A:HIS49
|
3.6
|
25.8
|
1.0
|
HA
|
A:TYR418
|
3.6
|
26.6
|
1.0
|
C
|
A:HIS49
|
3.6
|
22.2
|
1.0
|
CA
|
A:GLY367
|
3.7
|
22.2
|
1.0
|
CB
|
A:SER417
|
3.7
|
26.6
|
1.0
|
HA
|
A:ALA50
|
3.9
|
24.4
|
1.0
|
C
|
A:SER417
|
3.9
|
30.2
|
1.0
|
HG22
|
A:ILE369
|
3.9
|
24.3
|
1.0
|
HB2
|
A:TYR418
|
4.0
|
24.9
|
1.0
|
N
|
A:TYR418
|
4.0
|
25.2
|
1.0
|
O
|
A:SER417
|
4.1
|
31.1
|
1.0
|
HB2
|
A:HIS49
|
4.1
|
23.8
|
1.0
|
CA
|
A:TYR418
|
4.2
|
26.5
|
1.0
|
O
|
A:HOH924
|
4.3
|
36.1
|
1.0
|
N
|
A:HIS49
|
4.3
|
25.0
|
1.0
|
H
|
A:TYR418
|
4.4
|
26.6
|
1.0
|
CA
|
A:SER417
|
4.4
|
24.9
|
1.0
|
HD1
|
A:TYR418
|
4.5
|
25.8
|
1.0
|
CA
|
A:HIS49
|
4.5
|
24.8
|
1.0
|
HB2
|
A:SER417
|
4.5
|
27.9
|
1.0
|
HB2
|
A:ALA50
|
4.6
|
25.1
|
1.0
|
O
|
A:HOH962
|
4.6
|
25.2
|
1.0
|
HG21
|
A:ILE369
|
4.6
|
24.3
|
1.0
|
N
|
A:ALA50
|
4.6
|
22.7
|
1.0
|
O
|
A:HOH910
|
4.6
|
21.3
|
1.0
|
CB
|
A:TYR418
|
4.6
|
24.1
|
1.0
|
CA
|
A:ALA50
|
4.6
|
24.9
|
1.0
|
O
|
A:GLY368
|
4.7
|
23.3
|
1.0
|
N
|
A:GLY368
|
4.7
|
20.6
|
1.0
|
CG2
|
A:ILE369
|
4.7
|
25.5
|
1.0
|
O
|
A:HOH848
|
4.8
|
26.9
|
1.0
|
HB3
|
A:LYS48
|
4.8
|
34.4
|
1.0
|
O
|
A:HOH846
|
4.8
|
29.5
|
1.0
|
CB
|
A:HIS49
|
4.8
|
24.0
|
1.0
|
C
|
A:GLY368
|
4.8
|
21.2
|
1.0
|
HA
|
A:SER417
|
5.0
|
25.6
|
1.0
|
HA
|
A:ILE369
|
5.0
|
20.0
|
1.0
|
|
Sodium binding site 5 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 5 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 5 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Na601
b:31.5
occ:1.00
|
O
|
B:GLY466
|
2.3
|
25.1
|
1.0
|
O
|
B:HOH1067
|
2.4
|
35.8
|
1.0
|
OD1
|
B:ASP464
|
2.4
|
28.5
|
1.0
|
O
|
B:HOH1170
|
2.5
|
46.0
|
1.0
|
OG
|
B:SER518
|
2.5
|
31.0
|
1.0
|
O
|
B:HOH1127
|
2.6
|
36.0
|
1.0
|
HG
|
B:SER518
|
2.9
|
31.0
|
0.0
|
HB3
|
B:SER518
|
3.2
|
29.9
|
1.0
|
CG
|
B:ASP464
|
3.2
|
34.0
|
1.0
|
CB
|
B:SER518
|
3.3
|
29.7
|
1.0
|
O
|
B:HOH705
|
3.4
|
27.9
|
1.0
|
C
|
B:GLY466
|
3.4
|
25.2
|
1.0
|
H
|
B:GLY466
|
3.4
|
25.8
|
1.0
|
OD2
|
B:ASP464
|
3.4
|
36.3
|
1.0
|
HA2
|
B:GLY466
|
3.5
|
26.7
|
1.0
|
HB2
|
B:SER518
|
3.7
|
29.9
|
1.0
|
CA
|
B:GLY466
|
3.8
|
27.6
|
1.0
|
N
|
B:GLY466
|
3.9
|
26.1
|
1.0
|
O
|
B:HOH1175
|
4.0
|
43.1
|
1.0
|
OD1
|
B:ASP469
|
4.3
|
27.1
|
1.0
|
N
|
B:LEU467
|
4.6
|
26.0
|
1.0
|
CB
|
B:ASP464
|
4.6
|
25.9
|
1.0
|
HA
|
B:LEU467
|
4.6
|
25.1
|
1.0
|
CA
|
B:SER518
|
4.6
|
29.4
|
1.0
|
HA3
|
B:GLY466
|
4.7
|
26.7
|
1.0
|
HA
|
B:ASP464
|
4.8
|
25.6
|
1.0
|
O
|
B:HOH718
|
4.8
|
41.5
|
1.0
|
HB2
|
B:ASP464
|
4.9
|
27.5
|
1.0
|
C
|
B:SER518
|
4.9
|
32.9
|
1.0
|
O
|
B:SER518
|
5.0
|
33.7
|
1.0
|
H
|
B:LEU465
|
5.0
|
24.2
|
1.0
|
HA
|
B:SER518
|
5.0
|
29.4
|
1.0
|
|
Sodium binding site 6 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 6 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 6 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Na603
b:27.3
occ:1.00
|
O
|
B:HOH759
|
2.5
|
34.0
|
1.0
|
O
|
B:HOH1001
|
2.6
|
23.9
|
1.0
|
OE2
|
B:GLU533
|
2.6
|
22.7
|
1.0
|
OE1
|
B:GLU544
|
2.6
|
26.5
|
1.0
|
O
|
B:HOH753
|
2.8
|
37.4
|
1.0
|
HG2
|
B:GLU533
|
3.2
|
20.0
|
1.0
|
CD
|
B:GLU533
|
3.5
|
22.4
|
1.0
|
CD
|
B:GLU544
|
3.6
|
26.9
|
1.0
|
CG
|
B:GLU533
|
3.7
|
19.7
|
1.0
|
HA
|
B:HIS563
|
3.7
|
19.0
|
1.0
|
O
|
B:HOH1033
|
3.8
|
24.6
|
1.0
|
HG3
|
B:GLU533
|
3.8
|
19.9
|
1.0
|
OE2
|
B:GLU544
|
3.8
|
29.7
|
1.0
|
HD1
|
B:HIS563
|
3.9
|
21.9
|
0.0
|
O
|
B:HOH1147
|
4.0
|
40.4
|
1.0
|
O
|
H:HOH749
|
4.1
|
47.1
|
1.0
|
H
|
B:LEU564
|
4.1
|
18.8
|
1.0
|
O
|
B:PRO562
|
4.3
|
22.2
|
1.0
|
O
|
B:GLY534
|
4.5
|
21.3
|
1.0
|
OE1
|
B:GLU533
|
4.6
|
21.4
|
1.0
|
O
|
B:HOH1169
|
4.6
|
28.9
|
1.0
|
ND1
|
B:HIS563
|
4.7
|
21.9
|
1.0
|
O
|
B:HOH1231
|
4.7
|
29.1
|
1.0
|
CA
|
B:HIS563
|
4.7
|
18.3
|
1.0
|
O
|
B:HOH1240
|
4.8
|
28.9
|
1.0
|
O
|
B:HOH866
|
4.8
|
31.1
|
1.0
|
HB2
|
B:GLU544
|
4.9
|
23.1
|
1.0
|
HA2
|
B:GLY534
|
4.9
|
21.1
|
1.0
|
CG
|
B:GLU544
|
5.0
|
22.1
|
1.0
|
H
|
B:GLY534
|
5.0
|
20.4
|
1.0
|
N
|
B:LEU564
|
5.0
|
18.1
|
1.0
|
|
Sodium binding site 7 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 7 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 7 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Na601
b:45.8
occ:1.00
|
O
|
C:HOH1107
|
2.1
|
31.8
|
0.5
|
O
|
C:HOH982
|
2.2
|
42.1
|
1.0
|
O
|
C:HOH818
|
2.3
|
44.7
|
1.0
|
OG
|
C:SER518
|
2.6
|
40.7
|
1.0
|
O
|
C:SER518
|
2.6
|
39.9
|
1.0
|
O
|
C:HOH1067
|
2.7
|
45.5
|
1.0
|
HG
|
C:SER518
|
3.0
|
40.2
|
0.0
|
C
|
C:SER518
|
3.3
|
36.6
|
1.0
|
NA
|
C:NA602
|
3.4
|
38.7
|
1.0
|
CB
|
C:SER518
|
3.6
|
35.4
|
1.0
|
HB3
|
C:SER518
|
3.7
|
36.2
|
1.0
|
CA
|
C:SER518
|
3.8
|
34.0
|
1.0
|
HA
|
C:SER518
|
3.9
|
34.0
|
1.0
|
N
|
C:GLN519
|
4.2
|
39.8
|
1.0
|
HB3
|
C:GLN519
|
4.2
|
42.6
|
0.0
|
HA
|
C:GLN519
|
4.3
|
45.7
|
1.0
|
HB2
|
C:SER518
|
4.4
|
36.2
|
1.0
|
OD1
|
C:ASP469
|
4.5
|
31.0
|
1.0
|
OD2
|
C:ASP469
|
4.5
|
37.5
|
1.0
|
OD2
|
C:ASP464
|
4.6
|
42.1
|
1.0
|
CA
|
C:GLN519
|
4.7
|
48.1
|
1.0
|
CG
|
C:ASP464
|
4.8
|
34.5
|
1.0
|
OD1
|
C:ASP464
|
4.8
|
30.7
|
1.0
|
H
|
C:GLN519
|
4.9
|
40.5
|
1.0
|
CG
|
C:ASP469
|
4.9
|
32.9
|
1.0
|
|
Sodium binding site 8 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 8 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 8 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Na602
b:38.7
occ:1.00
|
O
|
C:HOH818
|
2.3
|
44.7
|
1.0
|
O
|
C:GLY466
|
2.4
|
30.2
|
1.0
|
OD1
|
C:ASP464
|
2.5
|
30.7
|
1.0
|
OG
|
C:SER518
|
2.5
|
40.7
|
1.0
|
O
|
C:HOH1067
|
2.5
|
45.5
|
1.0
|
HG
|
C:SER518
|
2.9
|
40.2
|
0.0
|
CG
|
C:ASP464
|
3.2
|
34.5
|
1.0
|
HB3
|
C:SER518
|
3.2
|
36.2
|
1.0
|
OD2
|
C:ASP464
|
3.3
|
42.1
|
1.0
|
CB
|
C:SER518
|
3.4
|
35.4
|
1.0
|
NA
|
C:NA601
|
3.4
|
45.8
|
1.0
|
C
|
C:GLY466
|
3.4
|
28.1
|
1.0
|
H
|
C:GLY466
|
3.4
|
28.1
|
1.0
|
HA2
|
C:GLY466
|
3.5
|
28.1
|
1.0
|
HB2
|
C:SER518
|
3.7
|
36.2
|
1.0
|
CA
|
C:GLY466
|
3.8
|
27.8
|
1.0
|
N
|
C:GLY466
|
4.0
|
28.7
|
1.0
|
OD1
|
C:ASP469
|
4.3
|
31.0
|
1.0
|
O
|
C:HOH1118
|
4.4
|
50.0
|
1.0
|
CB
|
C:ASP464
|
4.5
|
28.3
|
1.0
|
O
|
C:HOH982
|
4.5
|
42.1
|
1.0
|
N
|
C:LEU467
|
4.6
|
30.3
|
1.0
|
CA
|
C:SER518
|
4.7
|
34.0
|
1.0
|
HA
|
C:LEU467
|
4.8
|
30.7
|
1.0
|
HA3
|
C:GLY466
|
4.8
|
28.1
|
1.0
|
HB2
|
C:ASP464
|
4.8
|
29.3
|
1.0
|
HA
|
C:ASP464
|
4.8
|
27.0
|
1.0
|
O
|
C:HOH1107
|
5.0
|
31.8
|
0.5
|
|
Sodium binding site 9 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 9 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 9 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Na604
b:28.3
occ:1.00
|
O
|
C:HOH1105
|
2.2
|
51.8
|
1.0
|
O
|
C:HOH913
|
2.6
|
21.2
|
1.0
|
OE2
|
C:GLU533
|
2.6
|
21.2
|
1.0
|
O
|
C:HOH815
|
2.6
|
36.2
|
1.0
|
OE1
|
C:GLU544
|
2.6
|
26.9
|
1.0
|
O
|
C:HOH735
|
3.0
|
34.1
|
1.0
|
HG2
|
C:GLU533
|
3.1
|
20.6
|
1.0
|
CD
|
C:GLU533
|
3.5
|
22.7
|
1.0
|
CD
|
C:GLU544
|
3.6
|
24.9
|
1.0
|
O
|
C:HOH1133
|
3.7
|
40.7
|
1.0
|
O
|
C:HOH1153
|
3.7
|
49.7
|
1.0
|
CG
|
C:GLU533
|
3.7
|
20.3
|
1.0
|
HA
|
C:HIS563
|
3.8
|
18.7
|
1.0
|
OE2
|
C:GLU544
|
3.8
|
28.9
|
1.0
|
O
|
C:HOH990
|
3.8
|
25.4
|
1.0
|
HG3
|
C:GLU533
|
3.8
|
20.5
|
1.0
|
HD1
|
C:HIS563
|
3.8
|
21.8
|
0.0
|
O
|
C:HOH1088
|
4.0
|
34.5
|
1.0
|
H
|
C:LEU564
|
4.1
|
18.7
|
1.0
|
O
|
A:HOH813
|
4.2
|
52.7
|
1.0
|
O
|
C:PRO562
|
4.4
|
21.0
|
1.0
|
OE1
|
C:GLU533
|
4.6
|
22.1
|
1.0
|
O
|
C:GLY534
|
4.6
|
21.8
|
1.0
|
O
|
C:HOH1109
|
4.6
|
30.5
|
1.0
|
ND1
|
C:HIS563
|
4.7
|
21.8
|
1.0
|
O
|
C:HOH1148
|
4.7
|
28.4
|
1.0
|
O
|
C:HOH1158
|
4.7
|
29.7
|
1.0
|
CA
|
C:HIS563
|
4.8
|
18.1
|
1.0
|
HB2
|
C:GLU544
|
4.8
|
21.5
|
1.0
|
HA2
|
C:GLY534
|
4.9
|
20.0
|
1.0
|
O
|
C:HOH870
|
4.9
|
30.4
|
1.0
|
CG
|
C:GLU544
|
5.0
|
22.5
|
1.0
|
|
Sodium binding site 10 out
of 35 in 8pej
Go back to
Sodium Binding Sites List in 8pej
Sodium binding site 10 out
of 35 in the CJGH35 with A Galactosidase Activity-Based Probe
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 10 of CJGH35 with A Galactosidase Activity-Based Probe within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Na605
b:30.5
occ:1.00
|
O
|
C:HIS49
|
2.4
|
24.5
|
1.0
|
O
|
C:SER417
|
2.4
|
28.4
|
0.5
|
O
|
C:GLY367
|
2.5
|
23.1
|
1.0
|
O
|
C:HOH1047
|
2.8
|
28.0
|
1.0
|
OG
|
C:SER417
|
2.9
|
26.6
|
0.5
|
HG
|
C:SER417
|
3.0
|
31.8
|
0.0
|
O
|
C:HOH889
|
3.3
|
28.8
|
1.0
|
C
|
C:SER417
|
3.4
|
27.1
|
0.5
|
HA3
|
C:GLY367
|
3.4
|
21.4
|
1.0
|
C
|
C:GLY367
|
3.4
|
22.0
|
1.0
|
HG
|
C:SER417
|
3.5
|
26.1
|
0.0
|
HA2
|
C:GLY367
|
3.5
|
21.4
|
1.0
|
H
|
C:HIS49
|
3.5
|
25.4
|
1.0
|
HB3
|
C:SER417
|
3.5
|
26.1
|
0.5
|
C
|
C:HIS49
|
3.6
|
23.1
|
1.0
|
HA
|
C:TYR418
|
3.6
|
27.4
|
1.0
|
CA
|
C:GLY367
|
3.7
|
20.7
|
1.0
|
CB
|
C:SER417
|
3.7
|
26.1
|
0.5
|
OG
|
C:SER417
|
3.8
|
31.7
|
0.5
|
HB2
|
C:SER417
|
3.9
|
26.7
|
0.5
|
HA
|
C:ALA50
|
3.9
|
22.9
|
1.0
|
HG22
|
C:ILE369
|
4.0
|
25.2
|
1.0
|
C
|
C:SER417
|
4.0
|
27.3
|
0.5
|
N
|
C:TYR418
|
4.1
|
25.3
|
1.0
|
HB2
|
C:HIS49
|
4.2
|
24.1
|
1.0
|
HB2
|
C:TYR418
|
4.2
|
25.9
|
1.0
|
CB
|
C:SER417
|
4.2
|
25.3
|
0.5
|
O
|
C:HOH1076
|
4.2
|
31.6
|
1.0
|
N
|
C:HIS49
|
4.2
|
25.2
|
1.0
|
O
|
C:SER417
|
4.3
|
26.7
|
0.5
|
CA
|
C:TYR418
|
4.3
|
27.8
|
1.0
|
CA
|
C:SER417
|
4.4
|
25.4
|
0.5
|
CA
|
C:HIS49
|
4.4
|
24.1
|
1.0
|
HD1
|
C:TYR418
|
4.5
|
27.9
|
1.0
|
CA
|
C:SER417
|
4.5
|
25.7
|
0.5
|
O
|
C:HOH967
|
4.5
|
23.9
|
1.0
|
HB2
|
C:SER417
|
4.5
|
26.1
|
0.5
|
HG21
|
C:ILE369
|
4.5
|
25.2
|
1.0
|
N
|
C:ALA50
|
4.6
|
21.6
|
1.0
|
O
|
C:HOH986
|
4.6
|
21.6
|
1.0
|
O
|
C:GLY368
|
4.6
|
21.5
|
1.0
|
CA
|
C:ALA50
|
4.6
|
23.0
|
1.0
|
N
|
C:GLY368
|
4.6
|
20.1
|
1.0
|
H
|
C:TYR418
|
4.7
|
26.4
|
1.0
|
HB3
|
C:LYS48
|
4.7
|
33.7
|
1.0
|
CG2
|
C:ILE369
|
4.7
|
25.9
|
1.0
|
CB
|
C:TYR418
|
4.8
|
24.9
|
1.0
|
HB2
|
C:ALA50
|
4.8
|
23.5
|
1.0
|
O
|
C:HOH809
|
4.8
|
25.1
|
1.0
|
CB
|
C:HIS49
|
4.8
|
23.7
|
1.0
|
C
|
C:GLY368
|
4.8
|
22.0
|
1.0
|
HA
|
C:SER417
|
4.8
|
25.5
|
0.5
|
O
|
C:HOH866
|
4.8
|
28.5
|
1.0
|
HA
|
C:SER417
|
5.0
|
25.6
|
0.5
|
|
Reference:
C.L.Kuo,
Q.Su,
A.M.C.H.Van Den Nieuwendijk,
T.J.M.Beenakker,
W.A.Offen,
L.I.Willems,
R.G.Boot,
A.J.Sarris,
A.R.A.Marques,
J.D.C.Codee,
G.A.Van Der Marel,
B.I.Florea,
G.J.Davies,
H.S.Overkleeft,
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The Development of A Broad-Spectrum Retaining Beta-Exo-Galactosidase Activity-Based Probe. Org.Biomol.Chem. 2023.
ISSN: ESSN 1477-0539
PubMed: 37724332
DOI: 10.1039/D3OB01261A
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