Sodium in PDB 7za4: Gstf SH155 Mutant

All present enzymatic activity of Gstf SH155 Mutant:
2.5.1.18;

The structure of Gstf SH155 Mutant, PDB code: 7za4 was solved by A.C.Papageorgiou, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.31 / 2.05
Space group	I 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	46.478, 99.003, 153.212, 90, 93.73, 90
R / Rfree (%)	21.6 / 25.2

The binding sites of Sodium atom in the Gstf SH155 Mutant (pdb code 7za4). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Gstf SH155 Mutant, PDB code: 7za4:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Gstf SH155 Mutant


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Gstf SH155 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Na301 b:38.1 occ:1.00 O A:HOH321 2.3 31.8 1.0 OE1 B:GLU103 2.6 34.3 1.0 O A:HOH352 2.7 38.3 1.0 NE2 B:HIS107 2.9 42.4 1.0 CD B:GLU103 3.4 38.5 1.0 OE2 B:GLU103 3.5 38.1 1.0 CD2 B:HIS107 3.5 34.1 1.0 CB A:ALA106 3.5 29.8 1.0 CE1 B:HIS107 4.0 41.1 1.0 CD A:ARG69 4.0 33.8 1.0 NE2 A:HIS107 4.1 38.6 1.0 O B:HOH433 4.1 40.7 1.0 OE1 A:GLU103 4.2 34.3 1.0 CB B:ALA106 4.3 34.3 1.0 NE A:ARG69 4.3 30.3 1.0 O A:HOH319 4.4 33.5 1.0 OE2 A:GLU103 4.4 30.4 1.0 NH1 A:ARG69 4.5 33.9 1.0 CZ A:ARG69 4.6 34.6 1.0 CD A:GLU103 4.7 36.8 1.0 CE1 A:HIS107 4.7 41.2 1.0 CG B:HIS107 4.8 36.0 1.0 CG B:GLU103 4.8 36.9 1.0 CD2 A:HIS107 4.8 35.7 1.0 ND1 B:HIS107 5.0 38.7 1.0

Sodium binding site 2 out of 2 in the Gstf SH155 Mutant


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Gstf SH155 Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Na302 b:55.5 occ:1.00 O B:HOH443 2.1 58.9 1.0 ND1 B:HIS107 2.6 38.7 1.0 O A:HOH343 2.8 51.5 1.0 CE1 B:HIS107 3.5 41.1 1.0 CG B:HIS107 3.7 36.0 1.0 CB B:HIS107 4.0 34.5 1.0 CA B:HIS107 4.2 32.3 1.0 O A:HOH315 4.2 41.8 1.0 O B:HIS107 4.6 40.5 1.0 NE2 B:HIS107 4.7 42.4 1.0 CD2 B:HIS107 4.8 34.1 1.0 O A:ALA106 4.8 34.9 1.0 O B:ALA106 4.9 39.2 1.0 C B:HIS107 5.0 37.4 1.0 ND1 A:HIS107 5.0 38.0 1.0

E.Ioannou, A.C.Papageorgiou, N.E.Labrou. Directed Evolution of Phi Class Glutathione Transferases Involved in Multiple-Herbicide Resistance of Grass Weeds and Crops. Int J Mol Sci V. 23 2022.
ISSN: ESSN 1422-0067
PubMed: 35806486
DOI: 10.3390/IJMS23137469