Sodium in PDB 6yu7: Crystal Structure of Mhst in Complex with L-Tyrosine

The structure of Crystal Structure of Mhst in Complex with L-Tyrosine, PDB code: 6yu7 was solved by D.Focht, C.Neumann, J.Lyons, A.Eguskiza Bilbao, R.Blunck, L.Malinauskaite, I.O.Schwarz, J.A.Javitch, M.Quick, P.Nissen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.83 / 2.30
Space group	P 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	44.100, 49.900, 110.300, 90.00, 96.80, 90.00
R / Rfree (%)	22.3 / 26.3

The binding sites of Sodium atom in the Crystal Structure of Mhst in Complex with L-Tyrosine (pdb code 6yu7). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Mhst in Complex with L-Tyrosine, PDB code: 6yu7:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Mhst in Complex with L-Tyrosine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Mhst in Complex with L-Tyrosine within 5.0Å range: probe atom residue distance (Å) B Occ A:Na504 b:28.5 occ:1.00 O A:GLY24 2.1 25.9 1.0 O A:ALA320 2.2 26.4 1.0 O A:VAL27 2.2 29.3 1.0 OG A:SER323 2.4 31.1 1.0 OG A:SER324 2.4 30.9 1.0 O A:HOH609 2.6 33.1 1.0 N A:SER324 3.1 25.9 1.0 C A:ALA320 3.2 27.5 1.0 C A:GLY24 3.3 30.2 1.0 C A:VAL27 3.4 25.2 1.0 CB A:SER324 3.4 25.1 1.0 CB A:SER323 3.6 26.3 1.0 CA A:SER324 3.6 27.7 1.0 C A:SER323 3.6 19.4 1.0 CA A:ALA320 3.7 27.2 1.0 CA A:GLY24 4.0 29.9 1.0 CA A:SER323 4.0 23.8 1.0 CA A:GLY28 4.1 25.1 1.0 N A:GLY28 4.2 31.9 1.0 CB A:ALA320 4.2 26.4 1.0 N A:SER323 4.2 19.9 1.0 N A:SER25 4.4 25.9 1.0 N A:ALA321 4.4 26.4 1.0 O A:SER25 4.4 22.5 1.0 O A:SER323 4.4 28.5 1.0 CA A:VAL27 4.4 30.3 1.0 N A:VAL27 4.4 25.8 1.0 C A:SER25 4.5 26.3 1.0 CA A:SER25 4.6 27.1 1.0 O A:HOH646 4.6 39.8 1.0 CA A:ALA321 4.8 23.9 1.0 C A:ALA321 4.9 24.2 1.0 O A:ALA319 4.9 30.9 1.0 CB A:VAL27 4.9 33.3 1.0 O A:MET23 4.9 27.0 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Mhst in Complex with L-Tyrosine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Mhst in Complex with L-Tyrosine within 5.0Å range: probe atom residue distance (Å) B Occ A:Na505 b:29.1 occ:1.00 O A:ALA26 2.2 27.6 1.0 OXT A:TYR506 2.3 25.2 1.0 O A:THR231 2.4 29.1 1.0 OD1 A:ASN31 2.4 32.6 1.0 OG1 A:THR231 2.4 27.4 1.0 OD1 A:ASP263 2.4 29.8 1.0 C A:THR231 3.1 27.6 1.0 CA A:THR231 3.1 26.7 1.0 CG A:ASN31 3.2 27.5 1.0 C A:TYR506 3.2 21.8 1.0 C A:ALA26 3.2 27.2 1.0 CG A:ASP263 3.3 31.4 1.0 CB A:THR231 3.3 33.5 1.0 OD2 A:ASP263 3.3 27.7 1.0 ND2 A:ASN31 3.5 24.7 1.0 N A:TYR506 3.7 25.4 1.0 O A:TYR506 4.0 24.5 1.0 CA A:TYR506 4.0 23.4 1.0 N A:VAL27 4.1 25.8 1.0 CA A:VAL27 4.1 30.3 1.0 CA A:ALA26 4.1 27.6 1.0 N A:ASN31 4.1 27.8 1.0 N A:GLY28 4.2 31.9 1.0 CG2 A:THR231 4.4 27.3 1.0 N A:LEU232 4.4 24.2 1.0 CB A:ASN31 4.5 25.8 1.0 N A:THR231 4.5 27.9 1.0 C A:GLY30 4.5 29.7 1.0 CB A:ALA26 4.5 23.3 1.0 C A:VAL27 4.6 25.2 1.0 CA A:GLY30 4.6 28.5 1.0 CA A:ASN31 4.7 27.4 1.0 CB A:ASP263 4.7 31.1 1.0 O A:PHE230 4.8 27.9 1.0 O A:HOH633 4.9 32.7 1.0 N A:GLY30 4.9 23.9 1.0

D.Focht, C.Neumann, J.Lyons, A.Eguskiza Bilbao, R.Blunck, L.Malinauskaite, I.O.Schwarz, J.A.Javitch, M.Quick, P.Nissen. Role of A Non-Helical Region of Transmembrane Helix 6 in the Substrate Recognition Mechanism of the Hydrophobic Amino Acid Transporter Mhst. To Be Published.