Sodium in PDB 6yu3: Crystal Structure of Mhst in Complex with L-Phenylalanine

The structure of Crystal Structure of Mhst in Complex with L-Phenylalanine, PDB code: 6yu3 was solved by D.Focht, C.Neumann, J.Lyons, A.Eguskiza Bilbao, R.Blunck, L.Malinauskaite, I.O.Schwarz, J.A.Javitch, M.Quick, P.Nissen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.38 / 2.25
Space group	P 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	44.280, 49.890, 110.050, 90.00, 96.76, 90.00
R / Rfree (%)	22.4 / 25.5

The binding sites of Sodium atom in the Crystal Structure of Mhst in Complex with L-Phenylalanine (pdb code 6yu3). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Mhst in Complex with L-Phenylalanine, PDB code: 6yu3:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Mhst in Complex with L-Phenylalanine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Mhst in Complex with L-Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Na701 b:35.7 occ:1.00 O A:GLY24 2.2 34.6 1.0 O A:ALA320 2.2 28.6 1.0 O A:VAL27 2.3 32.7 1.0 OG A:SER323 2.4 31.8 1.0 OG A:SER324 2.4 39.9 1.0 O A:HOH818 2.7 47.8 1.0 N A:SER324 3.1 30.9 1.0 C A:ALA320 3.2 34.4 1.0 C A:GLY24 3.3 37.9 1.0 C A:VAL27 3.4 35.0 1.0 CB A:SER324 3.5 32.1 1.0 CA A:ALA320 3.6 32.0 1.0 CB A:SER323 3.6 34.8 1.0 CA A:SER324 3.7 33.9 1.0 C A:SER323 3.7 32.2 1.0 CB A:ALA320 4.0 32.4 1.0 CA A:GLY28 4.0 31.8 1.0 CA A:GLY24 4.1 33.0 1.0 CA A:SER323 4.1 35.5 1.0 N A:GLY28 4.2 36.9 1.0 N A:SER323 4.3 29.4 1.0 N A:SER25 4.4 34.2 1.0 N A:ALA321 4.4 31.0 1.0 CA A:VAL27 4.5 32.9 1.0 O A:SER25 4.5 32.8 1.0 O A:SER323 4.5 35.5 1.0 N A:VAL27 4.5 32.3 1.0 C A:SER25 4.5 36.4 1.0 CA A:SER25 4.6 31.6 1.0 CA A:ALA321 4.8 34.5 1.0 O A:ALA319 4.8 33.9 1.0 O A:HOH856 4.9 49.3 1.0 O A:MET23 4.9 37.6 1.0 N A:ALA320 4.9 31.7 1.0 CB A:VAL27 4.9 33.5 1.0 C A:ALA321 5.0 33.2 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Mhst in Complex with L-Phenylalanine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Mhst in Complex with L-Phenylalanine within 5.0Å range: probe atom residue distance (Å) B Occ A:Na702 b:33.0 occ:1.00 O A:ALA26 2.2 32.9 1.0 OD1 A:ASN31 2.4 36.0 1.0 OG1 A:THR231 2.4 35.0 1.0 OD1 A:ASP263 2.4 42.4 1.0 O A:THR231 2.4 35.0 1.0 OXT A:PHE703 2.5 31.1 1.0 CA A:THR231 3.1 32.5 1.0 CG A:ASN31 3.1 33.5 1.0 C A:THR231 3.2 32.2 1.0 C A:ALA26 3.3 33.6 1.0 CB A:THR231 3.3 33.3 1.0 CG A:ASP263 3.3 44.6 1.0 ND2 A:ASN31 3.4 33.7 1.0 C A:PHE703 3.4 30.8 1.0 OD2 A:ASP263 3.5 39.4 1.0 N A:PHE703 3.6 30.5 1.0 N A:VAL27 4.1 32.3 1.0 N A:ASN31 4.1 34.6 1.0 CA A:VAL27 4.1 32.9 1.0 N A:GLY28 4.1 36.9 1.0 CA A:ALA26 4.1 36.1 1.0 CA A:PHE703 4.2 33.5 1.0 O A:PHE703 4.3 34.5 1.0 CG2 A:THR231 4.4 30.0 1.0 CB A:ASN31 4.4 37.6 1.0 C A:GLY30 4.4 35.0 1.0 N A:LEU232 4.5 30.5 1.0 N A:THR231 4.5 35.4 1.0 C A:VAL27 4.6 35.0 1.0 CA A:GLY30 4.6 37.5 1.0 CB A:ALA26 4.6 34.4 1.0 CA A:ASN31 4.6 34.1 1.0 CB A:ASP263 4.7 35.5 1.0 O A:PHE230 4.8 32.4 1.0 N A:GLY30 4.9 32.1 1.0 O A:HOH834 4.9 43.5 1.0

D.Focht, C.Neumann, J.Lyons, A.Eguskiza Bilbao, R.Blunck, L.Malinauskaite, I.O.Schwarz, J.A.Javitch, M.Quick, P.Nissen. Role of A Non-Helical Region of Transmembrane Helix 6 in the Substrate Recognition Mechanism of the Hydrophobic Amino Acid Transporter Mhst. To Be Published.