Sodium in PDB 6seb: Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
Enzymatic activity of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
All present enzymatic activity of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg:
3.2.1.23;
Protein crystallography data
The structure of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg, PDB code: 6seb
was solved by
M.Rutkiewicz,
A.Bujacz,
P.Kaminska,
G.Bujacz,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
46.54 /
2.27
|
Space group
|
P 31 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
137.069,
137.069,
126.795,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
15.7 /
20.5
|
Sodium Binding Sites:
The binding sites of Sodium atom in the Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
(pdb code 6seb). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 4 binding sites of Sodium where determined in the
Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg, PDB code: 6seb:
Jump to Sodium binding site number:
1;
2;
3;
4;
Sodium binding site 1 out
of 4 in 6seb
Go back to
Sodium Binding Sites List in 6seb
Sodium binding site 1 out
of 4 in the Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1108
b:45.2
occ:1.00
|
OD2
|
A:ASP584
|
2.4
|
38.8
|
1.0
|
OD1
|
A:ASP207
|
2.4
|
42.3
|
1.0
|
O
|
A:HOH1662
|
2.5
|
35.8
|
1.0
|
O
|
A:PHE581
|
2.5
|
44.5
|
1.0
|
O6
|
A:IPT1101
|
2.6
|
52.8
|
0.7
|
CG
|
A:ASP207
|
3.3
|
44.0
|
1.0
|
CG
|
A:ASP584
|
3.4
|
43.5
|
1.0
|
C6
|
A:IPT1101
|
3.4
|
55.8
|
0.7
|
OD2
|
A:ASP207
|
3.4
|
44.4
|
1.0
|
C
|
A:PHE581
|
3.6
|
49.1
|
1.0
|
OH
|
A:TYR108
|
3.8
|
40.9
|
0.5
|
OH
|
A:TYR108
|
3.8
|
40.9
|
0.5
|
NE1
|
A:TRP548
|
3.9
|
36.4
|
1.0
|
OD1
|
A:ASP584
|
4.0
|
40.2
|
1.0
|
O4
|
A:IPT1101
|
4.2
|
41.2
|
0.7
|
CA
|
A:VAL582
|
4.3
|
48.3
|
1.0
|
N
|
A:VAL582
|
4.3
|
49.0
|
1.0
|
CB
|
A:PHE581
|
4.4
|
45.5
|
1.0
|
OE2
|
A:GLU549
|
4.4
|
32.1
|
1.0
|
CB
|
A:ASP584
|
4.5
|
33.4
|
1.0
|
CA
|
A:PHE581
|
4.5
|
45.1
|
1.0
|
CD2
|
A:PHE581
|
4.6
|
43.0
|
1.0
|
CB
|
A:ASP207
|
4.7
|
34.1
|
1.0
|
C5
|
A:IPT1101
|
4.7
|
58.4
|
0.7
|
NE2
|
A:HIS520
|
4.7
|
28.1
|
1.0
|
CE2
|
A:TRP548
|
4.8
|
33.6
|
1.0
|
CD1
|
A:TRP548
|
4.8
|
25.0
|
1.0
|
C4
|
A:IPT1101
|
4.8
|
53.8
|
0.7
|
O
|
A:ASP207
|
4.9
|
33.7
|
1.0
|
CZ2
|
A:TRP548
|
4.9
|
27.3
|
1.0
|
CZ
|
A:TYR108
|
4.9
|
42.2
|
0.5
|
CZ
|
A:TYR108
|
4.9
|
42.2
|
0.5
|
C
|
A:VAL582
|
5.0
|
43.7
|
1.0
|
|
Sodium binding site 2 out
of 4 in 6seb
Go back to
Sodium Binding Sites List in 6seb
Sodium binding site 2 out
of 4 in the Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1109
b:47.7
occ:1.00
|
O
|
A:HOH1283
|
2.8
|
35.2
|
1.0
|
N
|
A:VAL519
|
3.0
|
32.9
|
1.0
|
OH
|
A:TYR532
|
3.0
|
33.0
|
1.0
|
CB
|
A:TYR518
|
3.4
|
25.0
|
1.0
|
CA
|
A:TYR518
|
3.4
|
28.2
|
1.0
|
CA
|
A:TYR482
|
3.6
|
36.4
|
1.0
|
CZ
|
A:TYR532
|
3.6
|
40.9
|
1.0
|
C
|
A:TYR518
|
3.7
|
32.5
|
1.0
|
CA
|
A:VAL519
|
3.8
|
28.1
|
1.0
|
CD
|
A:PRO987
|
3.8
|
36.4
|
1.0
|
CE2
|
A:TYR532
|
3.8
|
35.8
|
1.0
|
CD1
|
A:TYR482
|
3.9
|
30.6
|
1.0
|
CB
|
A:TYR482
|
4.1
|
33.5
|
1.0
|
C
|
A:TYR482
|
4.2
|
40.5
|
1.0
|
O
|
A:ALA483
|
4.4
|
37.7
|
1.0
|
O
|
A:TYR482
|
4.4
|
38.0
|
1.0
|
CG
|
A:TYR518
|
4.4
|
29.5
|
1.0
|
CG
|
A:TYR482
|
4.5
|
32.1
|
1.0
|
CG
|
A:PRO987
|
4.5
|
39.5
|
1.0
|
N
|
A:TYR482
|
4.5
|
39.0
|
1.0
|
CD2
|
A:TYR518
|
4.6
|
32.9
|
1.0
|
CG1
|
A:VAL519
|
4.6
|
33.4
|
1.0
|
CE1
|
A:TYR532
|
4.6
|
31.7
|
1.0
|
N
|
A:HIS520
|
4.7
|
24.5
|
1.0
|
OG1
|
A:THR982
|
4.7
|
30.4
|
1.0
|
C
|
A:VAL519
|
4.8
|
33.0
|
1.0
|
N
|
A:TYR518
|
4.8
|
34.0
|
1.0
|
CB
|
A:VAL519
|
4.9
|
28.0
|
1.0
|
CE1
|
A:TYR482
|
4.9
|
34.2
|
1.0
|
O
|
A:TYR518
|
4.9
|
34.7
|
1.0
|
O
|
A:HOH1435
|
4.9
|
33.4
|
1.0
|
CD2
|
A:TYR532
|
4.9
|
33.6
|
1.0
|
N
|
A:ALA483
|
5.0
|
34.3
|
1.0
|
O
|
A:GLU517
|
5.0
|
28.4
|
1.0
|
|
Sodium binding site 3 out
of 4 in 6seb
Go back to
Sodium Binding Sites List in 6seb
Sodium binding site 3 out
of 4 in the Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1110
b:50.8
occ:1.00
|
OE1
|
A:GLN951
|
2.7
|
41.6
|
1.0
|
N
|
A:ALA763
|
2.9
|
35.3
|
1.0
|
OE1
|
A:GLU916
|
2.9
|
42.5
|
1.0
|
CB
|
A:ALA763
|
3.3
|
35.7
|
1.0
|
CD
|
A:GLN951
|
3.4
|
45.3
|
1.0
|
CD2
|
A:LEU954
|
3.5
|
32.7
|
1.0
|
CG
|
A:GLN951
|
3.5
|
41.8
|
1.0
|
CA
|
A:TRP762
|
3.7
|
38.3
|
1.0
|
CB
|
A:TRP762
|
3.7
|
37.2
|
1.0
|
CA
|
A:ALA763
|
3.7
|
38.4
|
1.0
|
C
|
A:TRP762
|
3.7
|
41.1
|
1.0
|
NH1
|
A:ARG947
|
3.7
|
42.3
|
1.0
|
O
|
A:HOH1610
|
3.8
|
38.0
|
1.0
|
CB
|
A:GLU916
|
3.9
|
28.3
|
1.0
|
NH2
|
A:ARG947
|
4.0
|
41.7
|
1.0
|
CD
|
A:GLU916
|
4.0
|
42.1
|
1.0
|
CB
|
A:GLN951
|
4.1
|
41.4
|
1.0
|
CA
|
A:GLU916
|
4.2
|
32.7
|
1.0
|
CZ
|
A:ARG947
|
4.2
|
49.3
|
1.0
|
O
|
A:ALA763
|
4.5
|
39.9
|
1.0
|
NH2
|
A:ARG863
|
4.6
|
29.4
|
1.0
|
CG
|
A:GLU916
|
4.6
|
31.4
|
1.0
|
CG
|
A:LEU954
|
4.6
|
43.9
|
1.0
|
CA
|
A:GLN951
|
4.6
|
42.6
|
1.0
|
C
|
A:ALA763
|
4.6
|
37.9
|
1.0
|
NE2
|
A:GLN951
|
4.7
|
43.6
|
1.0
|
N
|
A:GLU916
|
4.7
|
37.9
|
1.0
|
CG
|
A:TRP762
|
4.8
|
47.3
|
1.0
|
O
|
A:TRP762
|
4.9
|
36.1
|
1.0
|
|
Sodium binding site 4 out
of 4 in 6seb
Go back to
Sodium Binding Sites List in 6seb
Sodium binding site 4 out
of 4 in the Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 4 of Cold-Adapted Beta-D-Galactosidase From Arthrobacter Sp. 32CB in Complex with Iptg within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1111
b:56.4
occ:1.00
|
N
|
A:VAL946
|
2.9
|
45.2
|
1.0
|
O
|
A:GLY918
|
2.9
|
44.9
|
1.0
|
O
|
A:HOH1309
|
3.0
|
41.9
|
1.0
|
CG2
|
A:THR945
|
3.1
|
33.0
|
1.0
|
O
|
A:VAL946
|
3.3
|
48.6
|
1.0
|
CA
|
A:THR945
|
3.4
|
41.6
|
1.0
|
C
|
A:THR945
|
3.6
|
45.9
|
1.0
|
CB
|
A:MET907
|
3.7
|
42.1
|
1.0
|
C
|
A:GLY918
|
3.7
|
43.7
|
1.0
|
CE
|
A:MET907
|
3.8
|
37.9
|
1.0
|
CB
|
A:THR945
|
3.9
|
45.6
|
1.0
|
O
|
A:MET907
|
3.9
|
42.0
|
1.0
|
C
|
A:VAL946
|
4.0
|
46.0
|
1.0
|
CA
|
A:VAL946
|
4.0
|
45.5
|
1.0
|
NH1
|
A:ARG920
|
4.2
|
46.8
|
1.0
|
CA
|
A:GLY918
|
4.2
|
34.9
|
1.0
|
O
|
A:LEU944
|
4.4
|
40.3
|
1.0
|
C
|
A:MET907
|
4.4
|
42.3
|
1.0
|
O
|
A:HOH1503
|
4.4
|
41.7
|
1.0
|
CG
|
A:MET907
|
4.5
|
41.3
|
1.0
|
N
|
A:THR945
|
4.6
|
41.7
|
1.0
|
CA
|
A:MET907
|
4.6
|
47.3
|
1.0
|
CB
|
A:VAL946
|
4.6
|
43.0
|
1.0
|
N
|
A:ALA919
|
4.7
|
44.3
|
1.0
|
OG1
|
A:THR945
|
4.7
|
45.0
|
1.0
|
O
|
A:THR945
|
4.9
|
42.4
|
1.0
|
C
|
A:LEU944
|
4.9
|
43.5
|
1.0
|
SD
|
A:MET907
|
4.9
|
50.9
|
1.0
|
|
Reference:
M.Rutkiewicz,
A.Bujacz,
M.Wanarska,
A.Wierzbicka-Wos,
H.Cieslinski.
Active Site Architecture and Reaction Mechanism Determination of Cold Adapted Beta-D-Galactosidase Fromarthrobactersp. 32CB. Int J Mol Sci V. 20 2019.
ISSN: ESSN 1422-0067
PubMed: 31484304
DOI: 10.3390/IJMS20174301
Page generated: Tue Oct 8 13:26:26 2024
|