Sodium in PDB 6f11: Glic Mutant D86A

The structure of Glic Mutant D86A, PDB code: 6f11 was solved by H.D.Hu, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.95
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	181.820, 133.790, 160.160, 90.00, 102.29, 90.00
R / Rfree (%)	19.5 / 21.6

The structure of Glic Mutant D86A also contains other interesting chemical elements:

Chlorine

(Cl)

7 atoms

The binding sites of Sodium atom in the Glic Mutant D86A (pdb code 6f11). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 6 binding sites of Sodium where determined in the Glic Mutant D86A, PDB code: 6f11:
Jump to Sodium binding site number: 1; 2; 3; 4; 5; 6;

Sodium binding site 1 out of 6 in the Glic Mutant D86A


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Glic Mutant D86A within 5.0Å range: probe atom residue distance (Å) B Occ A:Na404 b:77.5 occ:1.00 O A:ILE71 2.6 93.9 1.0 O A:PRO68 3.2 0.8 1.0 N A:ILE73 3.6 84.0 1.0 C A:ILE71 3.7 95.0 1.0 CA A:TRP72 3.9 88.1 1.0 CD1 A:ILE73 4.1 93.5 1.0 C A:TRP72 4.2 90.9 1.0 CB A:ILE73 4.2 83.5 1.0 C A:PRO68 4.2 0.9 1.0 N A:TRP72 4.3 90.2 1.0 CG1 A:ILE73 4.4 85.2 1.0 CA A:ILE73 4.5 81.1 1.0 O A:GLU69 4.6 0.8 1.0 O A:ILE73 4.8 80.3 1.0 C A:GLU69 4.9 0.1 1.0 CA A:ILE71 4.9 93.9 1.0 N A:GLU69 4.9 0.4 1.0 CA A:GLU69 4.9 0.2 1.0

Sodium binding site 2 out of 6 in the Glic Mutant D86A


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Glic Mutant D86A within 5.0Å range: probe atom residue distance (Å) B Occ B:Na403 b:83.5 occ:1.00 O B:ILE71 2.4 82.4 1.0 O B:PRO68 3.0 93.2 1.0 N B:ILE73 3.6 74.0 1.0 C B:ILE71 3.6 81.6 1.0 CA B:TRP72 3.9 77.7 1.0 CD1 B:ILE73 4.1 84.9 1.0 C B:TRP72 4.1 80.5 1.0 C B:PRO68 4.1 93.3 1.0 CB B:ILE73 4.1 72.9 1.0 CG1 B:ILE73 4.2 74.2 1.0 N B:TRP72 4.2 78.3 1.0 CA B:ILE73 4.4 70.9 1.0 O B:GLU69 4.7 95.6 1.0 O B:ILE73 4.8 74.9 1.0 CA B:ILE71 4.8 76.7 1.0 CA B:GLU69 4.8 89.3 1.0 N B:GLU69 4.9 89.3 1.0 N B:ILE71 4.9 78.3 1.0 C B:GLU69 4.9 93.3 1.0

Sodium binding site 3 out of 6 in the Glic Mutant D86A


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Glic Mutant D86A within 5.0Å range: probe atom residue distance (Å) B Occ C:Na401 b:77.5 occ:1.00 OG1 C:THR226 3.8 76.7 1.0 OG1 D:THR226 3.9 74.9 1.0 OG1 B:THR226 4.1 73.9 1.0 OG1 E:THR226 4.2 71.7 1.0 OG1 A:THR226 4.3 79.2 1.0 CG2 C:THR226 4.8 69.9 1.0 CB C:THR226 4.9 72.5 1.0 O C:HOH507 5.0 53.7 1.0

Sodium binding site 4 out of 6 in the Glic Mutant D86A


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 4 of Glic Mutant D86A within 5.0Å range: probe atom residue distance (Å) B Occ C:Na404 b:89.1 occ:1.00 O C:ILE71 2.6 94.2 1.0 O C:PRO68 3.0 97.7 1.0 N C:ILE73 3.7 85.5 1.0 C C:ILE71 3.7 96.0 1.0 CD1 C:ILE73 4.0 91.7 1.0 C C:PRO68 4.0 97.3 1.0 CA C:TRP72 4.0 89.6 1.0 CB C:ILE73 4.2 84.6 1.0 C C:TRP72 4.2 91.7 1.0 CG1 C:ILE73 4.3 85.8 1.0 N C:TRP72 4.3 91.6 1.0 CA C:ILE73 4.5 82.5 1.0 O C:GLU69 4.7 99.9 1.0 CA C:GLU69 4.8 93.9 1.0 N C:GLU69 4.8 93.3 1.0 O C:ILE73 4.9 86.0 1.0 C C:GLU69 4.9 99.0 1.0 CA C:ILE71 5.0 95.1 1.0

Sodium binding site 5 out of 6 in the Glic Mutant D86A


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 5 of Glic Mutant D86A within 5.0Å range: probe atom residue distance (Å) B Occ D:Na403 b:0.8 occ:1.00 O D:ILE71 2.5 93.9 1.0 O D:PRO68 3.0 0.2 1.0 C D:ILE71 3.6 95.6 1.0 N D:ILE73 3.7 85.0 1.0 CA D:TRP72 3.9 91.2 1.0 CD1 D:ILE73 4.1 93.8 1.0 C D:PRO68 4.1 0.8 1.0 C D:TRP72 4.2 92.9 1.0 CB D:ILE73 4.2 83.8 1.0 N D:TRP72 4.2 92.6 1.0 CG1 D:ILE73 4.4 85.1 1.0 CA D:ILE73 4.5 81.9 1.0 O D:GLU69 4.7 0.9 1.0 CA D:GLU69 4.8 99.8 1.0 N D:GLU69 4.8 0.5 1.0 CA D:ILE71 4.9 92.6 1.0 C D:GLU69 4.9 0.4 1.0 O D:ILE73 4.9 79.7 1.0 N D:ILE71 4.9 94.3 1.0

Sodium binding site 6 out of 6 in the Glic Mutant D86A


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 6 of Glic Mutant D86A within 5.0Å range: probe atom residue distance (Å) B Occ E:Na403 b:95.9 occ:1.00 O E:ILE71 2.6 94.1 1.0 O E:PRO68 3.0 0.3 1.0 N E:ILE73 3.6 82.3 1.0 C E:ILE71 3.8 94.1 1.0 CA E:TRP72 4.0 87.8 1.0 CB E:ILE73 4.1 81.8 1.0 CD1 E:ILE73 4.1 90.0 1.0 C E:PRO68 4.1 0.6 1.0 C E:TRP72 4.2 88.4 1.0 CG1 E:ILE73 4.3 83.1 1.0 N E:TRP72 4.3 89.6 1.0 CA E:ILE73 4.4 79.7 1.0 O E:GLU69 4.8 0.5 1.0 O E:ILE73 4.8 82.9 1.0 N E:GLU69 4.9 0.8 1.0 CA E:GLU69 4.9 0.1 1.0 CA E:ILE71 5.0 90.5 1.0 C E:GLU69 5.0 0.8 1.0

A.Nemecz, H.Hu, Z.Fourati, C.Van Renterghem, M.Delarue, P.J.Corringer. Full Mutational Mapping of Titratable Residues Helps to Identify Proton-Sensors Involved in the Control of Channel Gating in the Gloeobacter Violaceus Pentameric Ligand-Gated Ion Channel. Plos Biol. V. 15 04470 2017.
ISSN: ESSN 1545-7885
PubMed: 29281623
DOI: 10.1371/JOURNAL.PBIO.2004470