Sodium in PDB 6f0m: Glic Mutant E35Q

The structure of Glic Mutant E35Q, PDB code: 6f0m was solved by H.D.Hu, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	21.71 / 2.65
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	182.250, 133.450, 160.570, 90.00, 102.51, 90.00
R / Rfree (%)	21.4 / 22.2

The structure of Glic Mutant E35Q also contains other interesting chemical elements:

Chlorine

(Cl)

7 atoms

The binding sites of Sodium atom in the Glic Mutant E35Q (pdb code 6f0m). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 6 binding sites of Sodium where determined in the Glic Mutant E35Q, PDB code: 6f0m:
Jump to Sodium binding site number: 1; 2; 3; 4; 5; 6;

Sodium binding site 1 out of 6 in the Glic Mutant E35Q


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Glic Mutant E35Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Na406 b:94.9 occ:1.00 O A:ILE71 2.4 83.5 1.0 O A:PRO68 2.8 0.9 1.0 C A:ILE71 3.6 84.3 1.0 C A:PRO68 3.8 0.2 1.0 N A:ILE73 3.8 77.8 1.0 CA A:TRP72 4.1 80.1 1.0 CB A:ILE73 4.2 77.9 1.0 CD1 A:ILE73 4.3 86.7 1.0 N A:TRP72 4.3 81.0 1.0 C A:TRP72 4.3 84.2 1.0 O A:GLU69 4.4 97.2 1.0 CA A:GLU69 4.4 96.3 1.0 CG1 A:ILE73 4.4 78.6 1.0 N A:GLU69 4.5 96.8 1.0 C A:GLU69 4.5 97.1 1.0 CA A:ILE73 4.7 75.6 1.0 N A:ILE71 4.7 85.0 1.0 CA A:ILE71 4.7 82.8 1.0 CA A:PRO68 4.9 98.2 1.0

Sodium binding site 2 out of 6 in the Glic Mutant E35Q


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Glic Mutant E35Q within 5.0Å range: probe atom residue distance (Å) B Occ B:Na405 b:90.7 occ:1.00 O B:ILE71 2.9 73.7 1.0 O B:PRO68 3.3 98.2 1.0 N B:ILE73 3.7 73.5 1.0 C B:ILE71 4.1 75.0 1.0 CB B:ILE73 4.1 75.0 1.0 CA B:TRP72 4.2 69.1 1.0 C B:PRO68 4.3 98.9 1.0 C B:TRP72 4.4 76.2 1.0 CD1 B:ILE73 4.5 88.7 1.0 CA B:ILE73 4.5 71.6 1.0 CG1 B:ILE73 4.5 77.7 1.0 N B:TRP72 4.6 71.2 1.0 O B:ILE73 4.6 73.1 1.0 CA B:GLU69 4.9 96.9 1.0 O B:GLU69 4.9 100.0 1.0 N B:GLU69 5.0 96.6 1.0

Sodium binding site 3 out of 6 in the Glic Mutant E35Q


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Glic Mutant E35Q within 5.0Å range: probe atom residue distance (Å) B Occ C:Na404 b:88.3 occ:1.00 O C:ILE71 2.6 88.9 1.0 O C:PRO68 3.1 93.9 1.0 N C:ILE73 3.6 78.4 1.0 C C:ILE71 3.7 88.2 1.0 CA C:TRP72 4.0 80.8 1.0 CB C:ILE73 4.1 79.1 1.0 C C:TRP72 4.2 84.2 1.0 C C:PRO68 4.2 93.8 1.0 CD1 C:ILE73 4.3 90.7 1.0 CG1 C:ILE73 4.3 80.8 1.0 N C:TRP72 4.3 82.5 1.0 CA C:ILE73 4.4 76.3 1.0 O C:ILE73 4.7 78.7 1.0 O C:GLU69 4.8 94.5 1.0 CA C:GLU69 4.8 90.3 1.0 N C:GLU69 4.9 90.1 1.0 C C:GLU69 4.9 93.3 1.0 CA C:ILE71 5.0 86.2 1.0 N C:ILE71 5.0 87.1 1.0

Sodium binding site 4 out of 6 in the Glic Mutant E35Q


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 4 of Glic Mutant E35Q within 5.0Å range: probe atom residue distance (Å) B Occ D:Na401 b:0.2 occ:1.00 OG1 D:THR226 3.3 69.9 1.0 OG1 C:THR226 4.0 81.0 1.0 OG1 E:THR226 4.1 70.8 1.0 CG2 D:THR226 4.4 58.5 1.0 CG2 C:THR226 4.4 71.4 1.0 CB D:THR226 4.5 67.3 1.0 CB C:THR226 4.8 75.5 1.0

Sodium binding site 5 out of 6 in the Glic Mutant E35Q


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 5 of Glic Mutant E35Q within 5.0Å range: probe atom residue distance (Å) B Occ D:Na406 b:0.2 occ:1.00 O D:ILE71 2.5 88.6 1.0 O D:PRO68 2.8 0.2 1.0 C D:ILE71 3.6 90.0 1.0 C D:PRO68 3.8 0.5 1.0 N D:ILE73 4.1 79.5 1.0 O D:GLU69 4.1 0.3 1.0 CA D:GLU69 4.2 0.7 1.0 CA D:TRP72 4.2 83.6 1.0 C D:GLU69 4.3 0.1 1.0 N D:TRP72 4.4 85.6 1.0 N D:GLU69 4.4 0.4 1.0 C D:TRP72 4.5 86.7 1.0 CB D:ILE73 4.5 79.6 1.0 CD1 D:ILE73 4.5 82.1 1.0 N D:ILE71 4.6 90.6 1.0 CG1 D:ILE73 4.7 80.2 1.0 CA D:ILE71 4.7 88.3 1.0 CA D:ILE73 4.9 76.8 1.0 CA D:PRO68 5.0 0.3 1.0

Sodium binding site 6 out of 6 in the Glic Mutant E35Q


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 6 of Glic Mutant E35Q within 5.0Å range: probe atom residue distance (Å) B Occ E:Na406 b:97.0 occ:1.00 O E:ILE71 2.8 90.0 1.0 O E:PRO68 2.9 0.1 1.0 C E:PRO68 3.9 1.0 1.0 C E:ILE71 3.9 90.7 1.0 N E:ILE73 4.0 82.3 1.0 CA E:GLU69 4.4 0.6 1.0 CB E:ILE73 4.4 83.4 1.0 CA E:TRP72 4.4 85.0 1.0 O E:GLU69 4.5 0.4 1.0 CD1 E:ILE73 4.5 97.8 1.0 N E:GLU69 4.5 0.7 1.0 C E:GLU69 4.6 0.2 1.0 C E:TRP72 4.6 87.2 1.0 CG1 E:ILE73 4.6 85.1 1.0 N E:TRP72 4.6 85.8 1.0 CA E:ILE73 4.8 80.3 1.0 N E:ILE71 4.9 92.0 1.0

A.Nemecz, H.Hu, Z.Fourati, C.Van Renterghem, M.Delarue, P.J.Corringer. Full Mutational Mapping of Titratable Residues Helps to Identify Proton-Sensors Involved in the Control of Channel Gating in the Gloeobacter Violaceus Pentameric Ligand-Gated Ion Channel. Plos Biol. V. 15 04470 2017.
ISSN: ESSN 1545-7885
PubMed: 29281623
DOI: 10.1371/JOURNAL.PBIO.2004470