Sodium in PDB 5j0s: Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
Enzymatic activity of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
All present enzymatic activity of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus:
2.7.7.7;
Protein crystallography data
The structure of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus, PDB code: 5j0s
was solved by
V.K.Batra,
S.H.Wilson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
50.00 /
2.00
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
54.560,
79.530,
54.890,
90.00,
105.68,
90.00
|
R / Rfree (%)
|
19.9 /
24.6
|
Sodium Binding Sites:
The binding sites of Sodium atom in the Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
(pdb code 5j0s). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 4 binding sites of Sodium where determined in the
Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus, PDB code: 5j0s:
Jump to Sodium binding site number:
1;
2;
3;
4;
Sodium binding site 1 out
of 4 in 5j0s
Go back to
Sodium Binding Sites List in 5j0s
Sodium binding site 1 out
of 4 in the Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na401
b:9.1
occ:1.00
|
O
|
A:THR101
|
2.2
|
11.5
|
1.0
|
O
|
A:ILE106
|
2.4
|
9.0
|
1.0
|
OP1
|
P:DG9
|
2.5
|
9.9
|
1.0
|
O
|
P:HOH114
|
2.5
|
9.2
|
1.0
|
O
|
A:VAL103
|
2.6
|
13.0
|
1.0
|
O
|
A:HOH753
|
2.6
|
13.6
|
1.0
|
C
|
A:THR101
|
3.2
|
11.7
|
1.0
|
C
|
A:ILE106
|
3.4
|
9.4
|
1.0
|
NA
|
A:NA403
|
3.4
|
25.4
|
1.0
|
C
|
A:VAL103
|
3.4
|
12.1
|
1.0
|
P
|
P:DG9
|
3.5
|
11.7
|
1.0
|
OP2
|
P:DG9
|
3.6
|
9.0
|
1.0
|
N
|
A:ILE106
|
3.8
|
7.4
|
1.0
|
CA
|
A:THR101
|
3.8
|
11.7
|
1.0
|
CA
|
A:SER104
|
4.0
|
10.9
|
1.0
|
N
|
A:VAL103
|
4.0
|
12.2
|
1.0
|
N
|
A:GLY105
|
4.0
|
8.7
|
1.0
|
CA
|
A:ILE106
|
4.0
|
8.0
|
1.0
|
N
|
A:SER104
|
4.1
|
12.0
|
1.0
|
O
|
P:HOH134
|
4.2
|
45.4
|
1.0
|
N
|
A:ARG102
|
4.3
|
13.9
|
1.0
|
C
|
A:ARG102
|
4.3
|
13.2
|
1.0
|
CB
|
A:THR101
|
4.3
|
11.5
|
1.0
|
N
|
A:GLY107
|
4.3
|
9.0
|
1.0
|
C
|
A:SER104
|
4.4
|
9.9
|
1.0
|
CA
|
A:VAL103
|
4.4
|
13.1
|
1.0
|
CB
|
A:ILE106
|
4.4
|
11.4
|
1.0
|
O3'
|
P:DC8
|
4.5
|
13.0
|
1.0
|
CA
|
A:GLY107
|
4.5
|
9.7
|
1.0
|
CA
|
A:ARG102
|
4.6
|
13.2
|
1.0
|
O5'
|
P:DG9
|
4.7
|
13.1
|
1.0
|
C
|
A:GLY105
|
4.7
|
7.5
|
1.0
|
O
|
A:ARG102
|
4.8
|
11.7
|
1.0
|
O
|
A:LEU100
|
4.8
|
11.8
|
1.0
|
O
|
A:HOH540
|
4.9
|
31.4
|
1.0
|
O
|
A:HOH712
|
5.0
|
17.8
|
1.0
|
CA
|
A:GLY105
|
5.0
|
7.4
|
1.0
|
|
Sodium binding site 2 out
of 4 in 5j0s
Go back to
Sodium Binding Sites List in 5j0s
Sodium binding site 2 out
of 4 in the Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na402
b:18.2
occ:1.00
|
O
|
A:VAL65
|
2.2
|
19.9
|
1.0
|
O
|
D:HOH116
|
2.3
|
16.6
|
1.0
|
O
|
A:LYS60
|
2.4
|
23.1
|
1.0
|
O
|
A:LEU62
|
2.6
|
18.6
|
1.0
|
O
|
A:HOH765
|
2.7
|
23.7
|
1.0
|
OP1
|
D:DC3
|
2.7
|
18.0
|
1.0
|
C
|
A:VAL65
|
3.2
|
17.7
|
1.0
|
C
|
A:LYS60
|
3.5
|
23.3
|
1.0
|
C
|
A:LEU62
|
3.5
|
20.7
|
1.0
|
N
|
A:VAL65
|
3.6
|
15.7
|
1.0
|
P
|
D:DC3
|
3.7
|
17.2
|
1.0
|
CA
|
A:VAL65
|
3.9
|
16.1
|
1.0
|
OP2
|
D:DC3
|
3.9
|
17.2
|
1.0
|
N
|
A:GLY64
|
4.0
|
19.0
|
1.0
|
CA
|
A:LYS60
|
4.1
|
21.3
|
1.0
|
CA
|
A:PRO63
|
4.2
|
20.1
|
1.0
|
N
|
A:LEU62
|
4.2
|
21.7
|
1.0
|
CB
|
A:VAL65
|
4.2
|
14.2
|
1.0
|
CG
|
A:LYS60
|
4.2
|
23.0
|
1.0
|
N
|
A:PRO63
|
4.2
|
20.8
|
1.0
|
C
|
A:LYS61
|
4.3
|
23.4
|
1.0
|
N
|
A:GLY66
|
4.3
|
17.1
|
1.0
|
N
|
A:LYS61
|
4.5
|
24.6
|
1.0
|
CA
|
A:LEU62
|
4.5
|
20.7
|
1.0
|
C
|
A:PRO63
|
4.5
|
18.9
|
1.0
|
O3'
|
D:DT2
|
4.5
|
16.4
|
1.0
|
O
|
A:LYS61
|
4.6
|
24.5
|
1.0
|
C
|
A:GLY64
|
4.6
|
16.3
|
1.0
|
CA
|
A:GLY66
|
4.6
|
16.5
|
1.0
|
CA
|
A:LYS61
|
4.7
|
23.8
|
1.0
|
CB
|
A:LYS60
|
4.8
|
22.1
|
1.0
|
O
|
A:ALA59
|
4.8
|
20.9
|
1.0
|
NZ
|
A:LYS60
|
4.8
|
24.6
|
1.0
|
CA
|
A:GLY64
|
5.0
|
16.6
|
1.0
|
CG1
|
A:VAL65
|
5.0
|
14.7
|
1.0
|
|
Sodium binding site 3 out
of 4 in 5j0s
Go back to
Sodium Binding Sites List in 5j0s
Sodium binding site 3 out
of 4 in the Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na403
b:25.4
occ:1.00
|
O
|
A:HOH888
|
2.3
|
22.8
|
1.0
|
O
|
A:HOH712
|
2.3
|
17.8
|
1.0
|
O
|
A:THR101
|
2.7
|
11.5
|
1.0
|
O
|
A:HOH846
|
2.7
|
22.2
|
1.0
|
O
|
A:HOH753
|
2.7
|
13.6
|
1.0
|
O
|
P:HOH114
|
2.7
|
9.2
|
1.0
|
NA
|
A:NA401
|
3.4
|
9.1
|
1.0
|
C
|
A:THR101
|
3.6
|
11.7
|
1.0
|
O
|
P:HOH134
|
3.9
|
45.4
|
1.0
|
CA
|
A:ARG102
|
4.0
|
13.2
|
1.0
|
N
|
A:ARG102
|
4.1
|
13.9
|
1.0
|
NZ
|
A:LYS27
|
4.3
|
31.2
|
1.0
|
CB
|
A:THR101
|
4.4
|
11.5
|
1.0
|
OG1
|
A:THR101
|
4.5
|
13.4
|
1.0
|
C
|
A:ARG102
|
4.5
|
13.2
|
1.0
|
CA
|
A:THR101
|
4.6
|
11.7
|
1.0
|
O
|
A:HOH896
|
4.6
|
22.2
|
1.0
|
O
|
A:HOH540
|
4.6
|
31.4
|
1.0
|
O
|
A:ARG102
|
4.8
|
11.7
|
1.0
|
O
|
A:HOH511
|
4.9
|
18.1
|
1.0
|
OP2
|
P:DG9
|
5.0
|
9.0
|
1.0
|
|
Sodium binding site 4 out
of 4 in 5j0s
Go back to
Sodium Binding Sites List in 5j0s
Sodium binding site 4 out
of 4 in the Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 4 of Binary Complex Crystal Structure of Dna Polymerase Beta with C:T Mismatch at the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na404
b:22.8
occ:1.00
|
O
|
A:HOH762
|
2.2
|
18.1
|
1.0
|
OG
|
A:SER171
|
2.3
|
12.4
|
1.0
|
O
|
A:HOH673
|
2.3
|
16.3
|
1.0
|
CB
|
A:SER171
|
3.5
|
15.4
|
1.0
|
N
|
A:SER171
|
3.9
|
14.1
|
1.0
|
O
|
A:HOH867
|
4.1
|
30.1
|
1.0
|
CA
|
A:SER171
|
4.3
|
14.8
|
1.0
|
O
|
A:HOH598
|
4.3
|
19.7
|
1.0
|
O
|
A:HOH865
|
4.5
|
38.7
|
1.0
|
OD1
|
A:ASP170
|
4.6
|
14.1
|
1.0
|
O
|
A:HOH642
|
5.0
|
21.1
|
1.0
|
C
|
A:SER171
|
5.0
|
16.5
|
1.0
|
|
Reference:
V.K.Batra,
W.A.Beard,
L.C.Pedersen,
S.H.Wilson.
Structures of Dna Polymerase Mispaired Dna Termini Transitioning to Pre-Catalytic Complexes Support An Induced-Fit Fidelity Mechanism. Structure V. 24 1863 2016.
ISSN: ISSN 0969-2126
PubMed: 27642161
DOI: 10.1016/J.STR.2016.08.006
Page generated: Mon Oct 7 21:45:31 2024
|