Sodium in PDB 5eb8: Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27

All present enzymatic activity of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27:
3.2.1.8;

The structure of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27, PDB code: 5eb8 was solved by P.Mahanta, A.Bhardwaj, V.S.Reddy, S.Ramakumar, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	88.38 / 2.22
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.270, 77.320, 176.750, 90.00, 90.00, 90.00
R / Rfree (%)	16.8 / 21.4

The structure of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Sodium atom in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 (pdb code 5eb8). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27, PDB code: 5eb8:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 within 5.0Å range: probe atom residue distance (Å) B Occ A:Na402 b:24.9 occ:1.00 O A:ASP302 2.1 20.4 1.0 O A:HOH724 2.2 33.2 1.0 O A:SER18 2.3 21.6 1.0 O A:LEU305 2.4 18.6 1.0 O A:HOH702 2.4 23.6 1.0 C A:ASP302 3.3 20.5 1.0 C A:LEU305 3.4 17.6 1.0 C A:SER18 3.5 20.9 1.0 CA A:ASP302 3.9 21.0 1.0 CA A:SER306 4.0 16.7 1.0 CB A:ASP302 4.0 21.5 1.0 N A:SER306 4.1 16.8 1.0 N A:LEU305 4.2 17.4 1.0 CA A:SER18 4.3 22.6 1.0 N A:ALA303 4.3 21.3 1.0 CA A:LEU305 4.4 17.4 1.0 O A:HOH501 4.4 26.4 1.0 N A:PHE19 4.5 19.1 1.0 CA A:ALA303 4.6 21.5 1.0 C A:ALA303 4.6 21.7 1.0 CB A:PHE19 4.7 17.7 1.0 CA A:PHE19 4.7 18.1 1.0 O A:ALA303 4.8 22.9 1.0 CG A:ASP302 4.8 22.9 1.0 CB A:SER306 4.9 16.4 1.0 C A:SER306 4.9 16.1 1.0 CD2 A:PHE19 5.0 17.4 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 within 5.0Å range: probe atom residue distance (Å) B Occ B:Na402 b:30.2 occ:1.00 O B:SER18 2.2 24.8 1.0 O B:ASP302 2.2 29.2 1.0 O B:HOH650 2.3 34.4 1.0 O B:LEU305 2.4 24.0 1.0 O B:HOH639 2.6 34.7 1.0 C B:ASP302 3.3 29.5 1.0 C B:SER18 3.4 24.4 1.0 C B:LEU305 3.5 23.3 1.0 CA B:ASP302 3.9 30.4 1.0 CA B:SER306 3.9 21.9 1.0 CB B:ASP302 3.9 30.8 1.0 N B:SER306 4.1 22.4 1.0 CA B:SER18 4.3 25.7 1.0 N B:PHE19 4.4 22.9 1.0 N B:LEU305 4.4 25.2 1.0 N B:ALA303 4.5 30.1 1.0 CA B:PHE19 4.5 21.9 1.0 CB B:PHE19 4.5 21.4 1.0 CA B:LEU305 4.5 23.6 1.0 CG B:ASP302 4.7 32.2 1.0 C B:SER306 4.8 20.7 1.0 CA B:ALA303 4.8 31.0 1.0 CB B:SER306 4.8 22.1 1.0 C B:ALA303 4.8 31.2 1.0 CD2 B:PHE19 4.9 21.9 1.0 O B:SER306 4.9 21.4 1.0 O B:GLU17 5.0 30.7 1.0

P.Mahanta, A.Bhardwaj, V.S.Reddy, S.Ramakumar. Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 To Be Published.