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Sodium in PDB 4wuc: N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition

Enzymatic activity of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition

All present enzymatic activity of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition:
5.99.1.3;

Protein crystallography data

The structure of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition, PDB code: 4wuc was solved by S.J.Hearnshaw, T.T.Chung, C.E.M.Stevenson, A.Maxwell, D.M.Lawson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 27.49 / 1.90
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 87.910, 140.880, 79.520, 90.00, 90.00, 90.00
R / Rfree (%) 18.4 / 22

Other elements in 4wuc:

The structure of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition also contains other interesting chemical elements:

Magnesium (Mg) 1 atom
Chlorine (Cl) 1 atom

Sodium Binding Sites:

The binding sites of Sodium atom in the N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition (pdb code 4wuc). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition, PDB code: 4wuc:
Jump to Sodium binding site number: 1; 2; 3;

Sodium binding site 1 out of 3 in 4wuc

Go back to Sodium Binding Sites List in 4wuc
Sodium binding site 1 out of 3 in the N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na403

b:27.8
occ:0.50
NA A:NA403 0.0 27.8 0.5
NA A:NA403 1.7 24.1 0.5
O A:VAL97 2.3 29.8 1.0
OG A:SER121 2.4 32.6 1.0
O A:ILE94 2.5 30.7 1.0
CB A:SER121 3.1 30.6 1.0
O A:GLY117 3.2 25.4 1.0
C A:VAL97 3.4 30.9 1.0
C A:ILE94 3.6 31.9 1.0
O2A A:ANP401 4.0 23.6 1.0
O A:ALA100 4.1 28.0 1.0
C A:GLY117 4.1 24.9 1.0
CA A:ILE94 4.1 31.6 1.0
N A:LEU98 4.2 30.9 1.0
CA A:LEU98 4.2 29.8 1.0
CA A:SER121 4.2 29.2 1.0
N A:VAL97 4.3 33.6 1.0
N A:SER121 4.3 27.8 1.0
CB A:ALA100 4.4 29.3 1.0
O A:VAL93 4.4 31.8 1.0
O A:VAL118 4.4 26.4 1.0
CA A:VAL97 4.4 32.5 1.0
CA A:VAL118 4.5 27.0 1.0
N A:ALA100 4.5 27.6 1.0
N A:VAL118 4.6 24.9 1.0
N A:HIS99 4.6 26.8 1.0
C A:VAL118 4.7 26.4 1.0
C A:LEU98 4.7 28.8 1.0
N A:MET95 4.7 31.9 1.0
CG2 A:ILE94 4.8 32.3 1.0
CA A:ALA100 4.8 28.2 1.0
C A:ALA100 4.8 28.3 1.0
C A:MET95 4.9 31.1 1.0

Sodium binding site 2 out of 3 in 4wuc

Go back to Sodium Binding Sites List in 4wuc
Sodium binding site 2 out of 3 in the N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na403

b:24.1
occ:0.50
NA A:NA403 0.0 24.1 0.5
NA A:NA403 1.7 27.8 0.5
O2A A:ANP401 2.7 23.6 1.0
O A:ILE94 2.9 30.7 1.0
O A:ALA100 3.0 28.0 1.0
O A:GLY117 3.1 25.4 1.0
OG A:SER121 3.3 32.6 1.0
C A:GLY117 3.6 24.9 1.0
C5' A:ANP401 3.6 25.1 1.0
C A:ILE94 3.8 31.9 1.0
CG2 A:ILE94 3.8 32.3 1.0
O A:VAL97 3.8 29.8 1.0
PA A:ANP401 3.8 25.9 1.0
CA A:ILE94 4.0 31.6 1.0
N A:VAL118 4.1 24.9 1.0
C A:ALA100 4.1 28.3 1.0
CB A:SER121 4.2 30.6 1.0
O5' A:ANP401 4.2 25.6 1.0
O3A A:ANP401 4.2 25.4 1.0
CA A:GLY117 4.3 25.5 1.0
CB A:ILE94 4.4 32.5 1.0
CA A:VAL118 4.4 27.0 1.0
N A:SER121 4.4 27.8 1.0
C A:VAL118 4.5 26.4 1.0
N A:ALA100 4.5 27.6 1.0
CB A:ALA100 4.5 29.3 1.0
CG1 A:VAL120 4.5 30.0 1.0
O A:VAL118 4.6 26.4 1.0
CA A:ALA100 4.6 28.2 1.0
CG1 A:ILE94 4.7 34.2 1.0
C4' A:ANP401 4.9 26.1 1.0
CA A:SER121 4.9 29.2 1.0
N A:GLY119 5.0 25.0 1.0

Sodium binding site 3 out of 3 in 4wuc

Go back to Sodium Binding Sites List in 4wuc
Sodium binding site 3 out of 3 in the N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of N-Terminal 43 kDa Fragment of the E. Coli Dna Gyrase B Subunit Grown From 100 Mm Nacl Condition within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na404

b:40.3
occ:1.00
O A:HOH589 2.2 39.5 1.0
O A:HOH542 2.2 36.4 1.0
O A:HOH537 2.4 39.8 1.0
O A:HOH581 2.4 41.9 1.0
O A:LYS103 2.6 31.9 1.0
O A:ASP105 2.8 38.1 1.0
C A:LYS103 3.5 30.7 1.0
C A:ASP105 3.7 40.0 1.0
CA A:LYS103 4.0 29.4 1.0
O A:PRO328 4.0 39.0 1.0
N A:ASP105 4.0 36.3 1.0
O A:HOH575 4.3 31.1 1.0
O A:HOH530 4.3 33.9 1.0
O A:TYR109 4.3 38.1 1.0
O A:HOH626 4.3 43.3 1.0
OG A:SER112 4.4 28.4 1.0
CA A:ASP105 4.4 39.9 1.0
N A:PHE104 4.4 31.3 1.0
OD1 A:ASP329 4.4 36.9 1.0
CB A:LYS103 4.4 27.9 1.0
C A:PHE104 4.5 34.6 1.0
N A:ASP106 4.5 43.4 1.0
CA A:ASP106 4.6 45.5 1.0
O A:HOH565 4.6 30.2 1.0
CA A:PHE104 4.6 31.8 1.0
CB A:ASP106 5.0 47.8 1.0

Reference:

S.J.Hearnshaw, T.T.Chung, C.E.M.Stevenson, A.Maxwell, D.M.Lawson. The Role of Monovalent Cations in the Atpase Reaction of Dna Gyrase Acta Crystallogr.,Sect.D V. 71 996 2015.
ISSN: ESSN 1399-0047
DOI: 10.1107/S1399004715002916
Page generated: Mon Oct 7 18:55:03 2024

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