Sodium in PDB 4p1q: Green Fluorescent Protein E222H Variant

The structure of Green Fluorescent Protein E222H Variant, PDB code: 4p1q was solved by M.Klein, Y.Carius, D.Auerbach, S.Franz, G.Jung, C.R.D.Lancaster, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.76 / 1.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.600, 62.200, 67.570, 90.00, 90.00, 90.00
R / Rfree (%)	13.6 / 17.2

The binding sites of Sodium atom in the Green Fluorescent Protein E222H Variant (pdb code 4p1q). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total only one binding site of Sodium was determined in the Green Fluorescent Protein E222H Variant, PDB code: 4p1q:

Sodium binding site 1 out of 1 in the Green Fluorescent Protein E222H Variant


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Green Fluorescent Protein E222H Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Na301 b:14.2 occ:1.00 O A:HOH496 2.4 14.0 1.0 O A:HOH495 2.5 11.9 1.0 O A:GLY174 2.5 6.7 1.0 C A:GLY174 3.4 7.6 1.0 CA A:GLY174 3.7 8.1 1.0 O A:HOH478 4.0 10.2 1.0 O A:SER175 4.1 8.0 1.0 O A:HOH422 4.2 17.6 1.0 OD1 A:ASN170 4.4 9.9 1.0 N A:SER175 4.4 7.7 1.0 CG A:ASN170 4.7 10.0 1.0 C A:SER175 4.8 7.9 1.0 N A:GLY174 4.9 8.8 1.0 CB A:ASN170 4.9 7.9 1.0 CA A:SER175 5.0 7.3 1.0

D.Auerbach, M.Klein, S.Franz, Y.Carius, C.R.Lancaster, G.Jung. Replacement of Highly Conserved E222 By the Photostable Non-Photoconvertible Histidine in Gfp. Chembiochem V. 15 1404 2014.
ISSN: ESSN 1439-7633
PubMed: 24919579
DOI: 10.1002/CBIC.201402075