Sodium in PDB 4o54: Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370)

All present enzymatic activity of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370):
5.3.1.1;

The structure of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370), PDB code: 4o54 was solved by S.Lara-Gonzalez, C.G.Benitez-Cardoza, L.G.Brieba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.83 / 1.90
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.829, 56.202, 105.877, 90.00, 90.00, 90.00
R / Rfree (%)	16.5 / 21.5

The binding sites of Sodium atom in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370) (pdb code 4o54). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370), PDB code: 4o54:
Jump to Sodium binding site number: 1; 2; 3;

Sodium binding site 1 out of 3 in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370)


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370) within 5.0Å range: probe atom residue distance (Å) B Occ A:Na301 b:13.8 occ:1.00 O A:VAL227 2.3 13.6 1.0 O A:ALA222 2.3 14.9 1.0 O A:HOH516 2.4 28.8 1.0 OG A:SER251 2.4 14.0 1.0 O A:CYS224 2.4 15.1 1.0 CB A:SER251 3.4 15.5 1.0 C A:VAL227 3.5 15.4 1.0 C A:ALA222 3.5 12.2 1.0 C A:CYS224 3.6 13.5 1.0 CA A:SER251 4.0 18.8 1.0 N A:VAL227 4.1 14.8 1.0 C A:ALA223 4.1 16.4 1.0 N A:CYS224 4.1 16.1 1.0 O A:HOH426 4.2 30.1 1.0 CA A:VAL227 4.2 11.7 1.0 O A:PRO225 4.2 13.4 1.0 CB A:VAL227 4.2 15.5 1.0 C A:PRO225 4.3 14.0 1.0 CA A:ALA222 4.3 12.2 1.0 CA A:PRO225 4.4 14.3 1.0 O A:ALA223 4.4 14.7 1.0 CE1 A:PHE4 4.4 16.1 1.0 N A:ALA223 4.4 12.8 1.0 N A:PRO225 4.4 15.2 1.0 CA A:CYS224 4.5 13.2 1.0 CA A:ALA223 4.5 14.4 1.0 N A:ASP228 4.5 11.9 1.0 O A:HOH507 4.6 30.1 1.0 OXT A:LYS252 4.6 36.2 1.0 N A:LYS252 4.6 22.5 1.0 O A:HOH547 4.7 22.3 1.0 O A:LEU221 4.7 13.0 1.0 CA A:ASP228 4.7 15.0 1.0 C A:SER251 4.8 20.5 1.0 CG1 A:VAL227 4.8 14.3 1.0 CD1 A:PHE4 4.9 19.0 1.0 N A:ASP226 4.9 11.1 1.0

Sodium binding site 2 out of 3 in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370)


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370) within 5.0Å range: probe atom residue distance (Å) B Occ A:Na302 b:19.1 occ:1.00 O A:GLY156 2.2 24.4 1.0 O A:HOH567 2.3 32.2 1.0 O A:HOH577 2.3 44.1 1.0 OD1 A:ASP159 2.4 21.3 1.0 OD2 A:ASP159 2.5 29.4 1.0 CG A:ASP159 2.8 25.4 1.0 C A:GLY156 3.5 23.1 1.0 CB A:ASP159 4.2 21.8 1.0 O A:HOH452 4.3 23.6 1.0 N A:LYS157 4.4 23.6 1.0 CA A:LYS157 4.4 22.1 1.0 CA A:GLY156 4.4 25.5 1.0 O A:HOH565 4.6 33.6 1.0 OD2 A:ASP160 4.7 16.3 1.0 OD1 A:ASP160 5.0 21.1 1.0 CG A:ASP160 5.0 16.0 1.0

Sodium binding site 3 out of 3 in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370)


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Phe Mutant (TVAG_497370) within 5.0Å range: probe atom residue distance (Å) B Occ A:Na303 b:26.6 occ:1.00 O A:HOH520 2.3 29.2 1.0 O A:GLY136 2.4 23.7 1.0 O A:HOH555 2.4 33.8 1.0 O A:HOH575 2.4 37.6 1.0 C A:GLY136 3.6 19.5 1.0 CA A:GLY136 4.4 19.3 1.0 O A:HOH488 4.4 29.5 1.0 OE2 A:GLU140 4.5 39.1 1.0 OE1 A:GLU140 4.5 30.8 1.0 N A:LYS137 4.6 18.5 1.0 CA A:LYS137 4.7 22.5 1.0 CD A:LYS137 4.7 35.1 1.0 CD A:GLU140 4.9 34.9 1.0 CE A:LYS137 5.0 38.6 1.0

S.Lara-Gonzalez, C.G.Benitez-Cardoza, L.G.Brieba. Engineering Mutants with Altered Dimer-Monomer Equilibrium Reveal the Existence of Stable Monomeric Triosephosphate Isomerases To Be Published.