Sodium in PDB 4o52: Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370)

All present enzymatic activity of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370):
5.3.1.1;

The structure of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370), PDB code: 4o52 was solved by S.Lara-Gonzalez, G.M.Montero-Moran, C.G.Benitez-Cardoza, L.G.Brieba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.31 / 1.95
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.806, 55.858, 104.940, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 20.9

The binding sites of Sodium atom in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370) (pdb code 4o52). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370), PDB code: 4o52:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370)


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370) within 5.0Å range: probe atom residue distance (Å) B Occ A:Na301 b:13.3 occ:1.00 O A:HOH491 2.3 31.0 1.0 O A:GLY156 2.3 23.6 1.0 O A:HOH490 2.3 24.6 1.0 O A:HOH497 2.4 23.7 1.0 OD2 A:ASP159 2.5 29.2 1.0 OD1 A:ASP159 2.5 21.7 1.0 CG A:ASP159 2.8 23.4 1.0 C A:GLY156 3.5 19.8 1.0 O A:HOH483 4.1 17.2 1.0 CB A:ASP159 4.3 19.9 1.0 CA A:LYS157 4.4 17.3 1.0 N A:LYS157 4.4 17.5 1.0 O A:HOH520 4.4 25.6 1.0 CA A:GLY156 4.5 18.5 1.0 OD2 A:ASP160 4.6 15.2 1.0 CD A:LYS157 4.7 20.9 1.0 CG A:ASP160 4.9 12.8 1.0 OD1 A:ASP160 5.0 9.7 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370)


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Trichomonas Vaginalis Triosephosphate Isomerase ILE45-Val Mutant (TVAG_497370) within 5.0Å range: probe atom residue distance (Å) B Occ A:Na302 b:10.5 occ:1.00 O A:CYS224 2.3 11.9 1.0 O A:ALA222 2.3 10.6 1.0 OG A:SER251 2.4 12.0 1.0 O A:VAL227 2.4 9.7 1.0 O A:HOH484 2.5 18.1 1.0 CB A:SER251 3.3 13.5 1.0 C A:CYS224 3.5 10.3 1.0 C A:ALA222 3.5 10.3 1.0 C A:VAL227 3.5 11.7 1.0 CA A:SER251 4.0 15.5 1.0 C A:ALA223 4.0 15.0 1.0 N A:CYS224 4.1 10.4 1.0 O A:PRO225 4.1 12.7 1.0 N A:VAL227 4.1 9.6 1.0 C A:PRO225 4.2 12.8 1.0 CA A:VAL227 4.2 9.3 1.0 CA A:PRO225 4.3 10.7 1.0 N A:PRO225 4.3 12.1 1.0 CB A:VAL227 4.3 10.4 1.0 O A:ALA223 4.4 13.3 1.0 N A:ALA223 4.4 10.5 1.0 CA A:ALA223 4.4 13.4 1.0 CA A:CYS224 4.4 13.0 1.0 CA A:ALA222 4.4 10.1 1.0 CE1 A:PHE4 4.5 12.4 1.0 N A:LYS252 4.6 23.9 1.0 N A:ASP228 4.6 11.1 1.0 C A:SER251 4.7 19.6 1.0 O A:HOH566 4.7 30.9 1.0 O A:LEU221 4.7 9.8 1.0 OXT A:LYS252 4.8 45.5 1.0 CA A:ASP228 4.9 12.4 1.0 N A:ASP226 4.9 10.3 1.0 CD1 A:PHE4 5.0 14.1 1.0

S.Lara-Gonzalez, G.M.Montero-Moran, C.G.Benitez-Cardoza, L.G.Brieba. Engineering Mutants with Altered Dimer-Monomer Equilibrium Reveal the Existence of Stable Monomeric Triosephosphate Isomerases To Be Published.