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Sodium in PDB 4fo6: Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus.

Enzymatic activity of Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus.

All present enzymatic activity of Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus.:
2.7.7.7;

Protein crystallography data

The structure of Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus., PDB code: 4fo6 was solved by R.A.Gosavi, A.F.Moon, T.A.Kunkel, L.C.Pedersen, K.Bebenek, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.03 / 2.01
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 55.780, 63.655, 141.470, 90.00, 90.00, 90.00
R / Rfree (%) 19 / 23.1

Other elements in 4fo6:

The structure of Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus. also contains other interesting chemical elements:

Magnesium (Mg) 1 atom
Manganese (Mn) 1 atom
Chlorine (Cl) 1 atom

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus. (pdb code 4fo6). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus., PDB code: 4fo6:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 4fo6

Go back to Sodium Binding Sites List in 4fo6
Sodium binding site 1 out of 2 in the Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus.


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na602

b:43.4
occ:1.00
O A:ILE302 2.4 45.9 1.0
O A:ILE305 2.8 49.9 1.0
O A:CYS300 2.9 58.3 1.0
OP1 D:DC3 3.2 43.2 1.0
C A:ILE302 3.3 51.3 1.0
OP2 D:DC3 3.6 46.7 1.0
C A:CYS300 3.7 55.1 1.0
C A:ILE305 3.8 45.5 1.0
P D:DC3 3.9 47.5 1.0
CA A:PRO303 3.9 47.4 1.0
N A:PRO303 4.0 54.2 1.0
N A:ILE305 4.1 39.0 1.0
N A:ILE302 4.1 52.7 1.0
C A:SER301 4.2 46.0 1.0
CA A:ILE302 4.3 50.1 1.0
N A:GLY304 4.3 42.8 1.0
O A:SER301 4.4 53.7 1.0
CA A:CYS300 4.4 50.5 1.0
CA A:ILE305 4.4 41.3 1.0
C A:PRO303 4.5 53.4 1.0
N A:SER301 4.6 58.2 1.0
CB A:ILE305 4.7 47.3 1.0
CA A:SER301 4.8 69.9 1.0
N A:GLY306 4.8 40.0 1.0
O A:ALA299 4.8 54.7 1.0
O3' D:DC2 4.9 37.2 1.0
CA A:GLY306 5.0 37.0 1.0

Sodium binding site 2 out of 2 in 4fo6

Go back to Sodium Binding Sites List in 4fo6
Sodium binding site 2 out of 2 in the Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus.


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of the Pre-Catalytic Ternary Complex of Polymerase Lambda with A Datp Analog Opposite A Templating T and An Rcmp at the Primer Terminus. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na605

b:18.2
occ:1.00
O A:ALA344 2.4 18.9 1.0
O A:SER339 2.4 18.6 1.0
O A:ILE341 2.4 15.4 1.0
O A:HOH704 2.4 22.2 1.0
O A:HOH728 2.4 28.8 1.0
OP1 P:DA5 2.5 17.8 1.0
C A:ILE341 3.3 22.5 1.0
C A:SER339 3.4 23.7 1.0
P P:DA5 3.5 19.1 1.0
C A:ALA344 3.5 18.4 1.0
OP2 P:DA5 3.7 18.4 1.0
N A:ALA344 3.8 14.0 1.0
CA A:TRP342 3.9 15.2 1.0
N A:TRP342 4.0 19.1 1.0
N A:ILE341 4.0 15.6 1.0
N A:GLY343 4.0 16.4 1.0
C A:ASN340 4.1 22.2 1.0
CA A:ALA344 4.2 14.6 1.0
CA A:SER339 4.2 24.7 1.0
N A:ASN340 4.3 21.5 1.0
CA A:ASN340 4.3 21.5 1.0
C A:TRP342 4.3 19.8 1.0
CA A:ILE341 4.3 18.5 1.0
O3' P:DT4 4.4 16.1 1.0
N A:GLY345 4.5 18.1 1.0
O A:ASN340 4.6 21.6 1.0
CB A:ALA344 4.6 16.8 1.0
O5' P:DA5 4.7 14.8 1.0
C A:GLY343 4.7 13.2 1.0
CA A:GLY345 4.8 13.4 1.0
CB A:SER339 4.8 24.3 1.0
CA A:GLY343 4.9 20.0 1.0

Reference:

R.A.Gosavi, A.F.Moon, T.A.Kunkel, L.C.Pedersen, K.Bebenek. The Catalytic Cycle For Ribonucleotide Incorporation By Human Dna Pol Lambda Nucleic Acids Res. V. 40 1 2012.
ISSN: ISSN 0305-1048
PubMed: 22584622
DOI: 10.1093/NAR/GKS413
Page generated: Tue Dec 15 06:40:41 2020

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