Sodium in PDB 3qs6: Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan

The structure of Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan, PDB code: 3qs6 was solved by C.L.Piscitelli, E.Gouaux, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.49 / 2.80
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	91.793, 87.127, 82.169, 90.00, 94.73, 90.00
R / Rfree (%)	20 / 23.7

The binding sites of Sodium atom in the Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan (pdb code 3qs6). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan, PDB code: 3qs6:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan within 5.0Å range: probe atom residue distance (Å) B Occ A:Na751 b:35.1 occ:1.00 OG1 A:THR354 2.1 33.3 1.0 O A:VAL23 2.2 47.0 1.0 O A:ALA351 2.2 39.8 1.0 O A:GLY20 2.2 47.0 1.0 OG A:SER355 2.6 43.2 1.0 C A:VAL23 3.0 37.0 1.0 CB A:THR354 3.2 35.1 1.0 N A:SER355 3.3 42.7 1.0 C A:ALA351 3.4 41.2 1.0 C A:GLY20 3.4 45.5 1.0 C A:THR354 3.5 41.2 1.0 CB A:SER355 3.6 37.7 1.0 CA A:GLY24 3.7 36.7 1.0 N A:GLY24 3.7 31.8 1.0 CA A:THR354 3.8 39.3 1.0 CA A:SER355 3.8 36.5 1.0 CA A:ALA351 4.0 40.1 1.0 CA A:VAL23 4.1 40.3 1.0 CA A:GLY20 4.1 42.9 1.0 N A:THR354 4.1 36.2 1.0 N A:VAL23 4.1 47.1 1.0 O A:THR354 4.1 37.3 1.0 O A:ASN21 4.1 48.6 1.0 C A:ASN21 4.4 44.2 1.0 N A:ASN21 4.4 42.4 1.0 CG2 A:THR354 4.4 41.3 1.0 N A:GLY352 4.5 34.1 1.0 O A:PHE350 4.5 38.0 1.0 CB A:VAL23 4.6 38.3 1.0 CA A:ASN21 4.7 36.7 1.0 CA A:GLY352 4.8 37.7 1.0 C A:ALA22 4.8 45.2 1.0 C A:GLY352 4.8 37.6 1.0 CB A:ALA351 4.8 32.2 1.0 O A:GLY352 4.9 42.8 1.0 O A:ALA19 4.9 43.6 1.0 N A:ALA22 5.0 37.6 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Leut Mutant F259V,I359Q Bound to Sodium and L- Tryptophan within 5.0Å range: probe atom residue distance (Å) B Occ A:Na752 b:58.0 occ:1.00 OD1 A:ASN27 2.1 48.0 1.0 O A:ALA22 2.2 35.9 1.0 OG1 A:THR254 2.4 50.1 1.0 OXT A:TRP601 2.4 51.6 1.0 ND2 A:ASN286 2.4 47.7 1.0 OD1 A:ASN286 2.4 41.3 1.0 CG A:ASN286 2.8 44.4 1.0 O A:THR254 2.8 39.0 1.0 CG A:ASN27 2.9 46.0 1.0 ND2 A:ASN27 3.1 47.1 1.0 C A:ALA22 3.2 45.2 1.0 C A:THR254 3.4 41.6 1.0 CB A:THR254 3.4 40.1 1.0 CA A:THR254 3.4 47.3 1.0 C A:TRP601 3.6 42.7 1.0 OE2 A:GLU290 3.7 56.3 1.0 CA A:VAL23 3.9 40.3 1.0 N A:VAL23 3.9 47.1 1.0 N A:GLY24 4.2 31.8 1.0 CA A:ALA22 4.2 41.7 1.0 CB A:ASN286 4.3 50.6 1.0 CB A:ALA22 4.3 37.8 1.0 CB A:ASN27 4.3 45.1 1.0 N A:TRP601 4.3 45.3 1.0 O A:TRP601 4.3 37.8 1.0 N A:ASN27 4.5 41.3 1.0 CA A:TRP601 4.5 43.0 1.0 N A:LEU255 4.5 38.1 1.0 CG2 A:THR254 4.6 46.9 1.0 C A:VAL23 4.6 37.0 1.0 CA A:ASN27 4.7 48.3 1.0 CD A:GLU290 4.8 55.5 1.0 N A:THR254 4.8 49.9 1.0

C.L.Piscitelli, E.Gouaux. Insights Into Transport Mechanism From Leut Engineered to Transport Tryptophan. Embo J. V. 31 228 2012.
ISSN: ISSN 0261-4189
PubMed: 21952050
DOI: 10.1038/EMBOJ.2011.353