Sodium in PDB 3qs4: Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan

The structure of Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan, PDB code: 3qs4 was solved by C.L.Piscitelli, E.Gouaux, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.02 / 2.63
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	90.219, 86.595, 82.058, 90.00, 93.63, 90.00
R / Rfree (%)	21.4 / 22.8

The binding sites of Sodium atom in the Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan (pdb code 3qs4). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan, PDB code: 3qs4:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan within 5.0Å range: probe atom residue distance (Å) B Occ A:Na751 b:51.6 occ:1.00 OG1 A:THR354 2.1 52.5 1.0 O A:VAL23 2.2 59.4 1.0 O A:ALA351 2.2 53.2 1.0 O A:GLY20 2.2 54.4 1.0 OG A:SER355 2.6 55.9 1.0 CB A:THR354 3.2 52.7 1.0 C A:ALA351 3.3 55.4 1.0 N A:SER355 3.4 50.1 1.0 C A:VAL23 3.4 53.9 1.0 C A:GLY20 3.4 59.2 1.0 C A:THR354 3.5 49.2 1.0 CB A:SER355 3.7 49.5 1.0 CA A:THR354 3.7 52.6 1.0 CA A:ALA351 3.8 56.0 1.0 CA A:SER355 3.9 51.8 1.0 N A:THR354 4.0 55.4 1.0 O A:THR354 4.0 54.2 1.0 O A:PHE350 4.1 54.2 1.0 CA A:GLY20 4.2 49.8 1.0 N A:GLY24 4.3 52.0 1.0 CA A:GLY24 4.3 54.6 1.0 CA A:VAL23 4.3 57.4 1.0 N A:GLY352 4.4 46.5 1.0 N A:ASN21 4.4 55.8 1.0 CG2 A:THR354 4.4 55.3 1.0 N A:VAL23 4.5 53.3 1.0 O A:ASN21 4.5 60.1 1.0 O A:GLY352 4.5 49.6 1.0 CB A:VAL23 4.5 65.5 1.0 C A:ASN21 4.6 58.5 1.0 CA A:ASN21 4.6 56.8 1.0 C A:GLY352 4.6 57.5 1.0 CA A:GLY352 4.8 52.9 1.0 CB A:ALA351 4.8 48.2 1.0 CG1 A:VAL23 4.9 58.1 1.0 N A:ALA351 4.9 54.3 1.0 C A:PHE350 5.0 53.7 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Leut Mutant F259V Bound to Sodium and L- Tryptophan within 5.0Å range: probe atom residue distance (Å) B Occ A:Na752 b:65.9 occ:1.00 OD1 A:ASN27 2.1 64.7 1.0 O A:ALA22 2.2 49.5 1.0 OG1 A:THR254 2.4 58.3 1.0 OXT A:TRP601 2.4 55.1 1.0 OD1 A:ASN286 2.4 54.2 1.0 O A:THR254 2.8 56.5 1.0 CG A:ASN27 2.9 59.6 1.0 ND2 A:ASN27 3.0 55.6 1.0 CG A:ASN286 3.2 56.7 1.0 C A:ALA22 3.2 57.0 1.0 CB A:THR254 3.4 60.5 1.0 CA A:THR254 3.4 60.7 1.0 ND2 A:ASN286 3.4 51.3 1.0 C A:THR254 3.4 60.2 1.0 C A:TRP601 3.6 53.8 1.0 O A:HOH826 3.6 62.0 1.0 CA A:VAL23 3.8 57.4 1.0 OE1 A:GLU290 3.8 69.8 1.0 N A:VAL23 3.9 53.3 1.0 N A:TRP601 4.0 54.8 1.0 N A:GLY24 4.0 52.0 1.0 CB A:ASN27 4.3 58.2 1.0 CA A:ALA22 4.3 56.3 1.0 O A:TRP601 4.4 55.0 1.0 C A:VAL23 4.4 53.9 1.0 CA A:TRP601 4.4 54.5 1.0 CB A:ALA22 4.6 53.8 1.0 CG2 A:THR254 4.6 56.4 1.0 N A:ASN27 4.7 59.4 1.0 CB A:ASN286 4.7 56.8 1.0 N A:LEU255 4.7 56.2 1.0 CA A:ASN27 4.8 59.5 1.0 N A:THR254 4.8 59.1 1.0 CD A:GLU290 4.9 70.9 1.0

C.L.Piscitelli, E.Gouaux. Insights Into Transport Mechanism From Leut Engineered to Transport Tryptophan. Embo J. V. 31 228 2012.
ISSN: ISSN 0261-4189
PubMed: 21952050
DOI: 10.1038/EMBOJ.2011.353