Sodium in PDB 2wp9: Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant
Enzymatic activity of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant
All present enzymatic activity of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant:
1.3.5.1;
1.3.99.1;
Protein crystallography data
The structure of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant, PDB code: 2wp9
was solved by
J.Ruprecht,
V.Yankovskaya,
E.Maklashina,
S.Iwata,
G.Cecchini,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
48.85 /
2.70
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
119.853,
183.803,
202.777,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
19 /
22.2
|
Other elements in 2wp9:
The structure of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant also contains other interesting chemical elements:
Sodium Binding Sites:
The binding sites of Sodium atom in the Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant
(pdb code 2wp9). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the
Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant, PDB code: 2wp9:
Jump to Sodium binding site number:
1;
2;
3;
Sodium binding site 1 out
of 3 in 2wp9
Go back to
Sodium Binding Sites List in 2wp9
Sodium binding site 1 out
of 3 in the Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant
 Mono view
 Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1590
b:31.6
occ:1.00
|
O
|
A:ALA390
|
2.3
|
41.4
|
1.0
|
O
|
A:GLY358
|
2.5
|
42.1
|
1.0
|
O
|
A:GLU388
|
2.5
|
38.8
|
1.0
|
O
|
A:MET356
|
2.7
|
41.0
|
1.0
|
O
|
A:MET357
|
3.1
|
42.9
|
1.0
|
C
|
A:GLU388
|
3.5
|
38.4
|
1.0
|
C
|
A:MET357
|
3.5
|
42.9
|
1.0
|
C
|
A:GLY358
|
3.5
|
43.3
|
1.0
|
C
|
A:ALA390
|
3.5
|
41.6
|
1.0
|
C
|
A:MET356
|
3.8
|
41.7
|
1.0
|
CA
|
A:GLU388
|
4.0
|
38.4
|
1.0
|
OH
|
A:TYR355
|
4.0
|
47.6
|
1.0
|
N
|
A:GLY358
|
4.0
|
43.2
|
1.0
|
CA
|
A:MET357
|
4.1
|
42.6
|
1.0
|
N
|
A:ALA390
|
4.1
|
40.4
|
1.0
|
O
|
A:GLY387
|
4.2
|
38.4
|
1.0
|
CA
|
A:GLY358
|
4.3
|
43.6
|
1.0
|
C
|
A:ILE389
|
4.4
|
40.0
|
1.0
|
N
|
A:CYS391
|
4.4
|
42.2
|
1.0
|
N
|
A:MET357
|
4.4
|
42.4
|
1.0
|
CA
|
A:CYS391
|
4.4
|
43.4
|
1.0
|
N
|
A:GLY359
|
4.5
|
43.1
|
1.0
|
CA
|
A:ALA390
|
4.5
|
41.0
|
1.0
|
N
|
A:ILE389
|
4.5
|
38.0
|
1.0
|
CG
|
A:GLU388
|
4.5
|
39.5
|
1.0
|
CA
|
A:GLY359
|
4.6
|
42.9
|
1.0
|
CZ
|
A:TYR355
|
4.6
|
43.5
|
1.0
|
CB
|
A:CYS391
|
4.7
|
43.9
|
1.0
|
CE2
|
A:TYR355
|
4.7
|
42.3
|
1.0
|
O
|
A:ILE389
|
4.8
|
40.5
|
1.0
|
CG
|
A:MET356
|
4.8
|
42.1
|
1.0
|
CE
|
A:MET356
|
4.8
|
40.9
|
1.0
|
CA
|
A:ILE389
|
4.8
|
38.9
|
1.0
|
CB
|
A:GLU388
|
4.9
|
37.8
|
1.0
|
|
Sodium binding site 2 out
of 3 in 2wp9
Go back to
Sodium Binding Sites List in 2wp9
Sodium binding site 2 out
of 3 in the Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant
 Mono view
 Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Na1590
b:37.8
occ:1.00
|
O
|
E:GLY358
|
2.3
|
51.4
|
1.0
|
O
|
E:MET356
|
2.5
|
49.7
|
1.0
|
O
|
E:ALA390
|
2.5
|
49.2
|
1.0
|
O
|
E:GLU388
|
2.6
|
47.6
|
1.0
|
O
|
E:MET357
|
2.7
|
51.5
|
1.0
|
C
|
E:MET357
|
3.2
|
51.7
|
1.0
|
C
|
E:GLY358
|
3.3
|
52.9
|
1.0
|
C
|
E:GLU388
|
3.6
|
47.2
|
1.0
|
C
|
E:MET356
|
3.6
|
50.9
|
1.0
|
C
|
E:ALA390
|
3.7
|
49.3
|
1.0
|
N
|
E:GLY358
|
3.8
|
52.6
|
1.0
|
CA
|
E:MET357
|
3.8
|
51.2
|
1.0
|
CA
|
E:GLY358
|
4.1
|
53.2
|
1.0
|
OH
|
E:TYR355
|
4.1
|
54.3
|
1.0
|
CA
|
E:GLU388
|
4.2
|
46.6
|
1.0
|
N
|
E:MET357
|
4.2
|
51.5
|
1.0
|
N
|
E:GLY359
|
4.3
|
53.2
|
1.0
|
N
|
E:ALA390
|
4.4
|
48.5
|
1.0
|
CA
|
E:CYS391
|
4.5
|
50.8
|
1.0
|
N
|
E:CYS391
|
4.5
|
49.9
|
1.0
|
O
|
E:GLY387
|
4.5
|
45.8
|
1.0
|
C
|
E:ILE389
|
4.5
|
49.0
|
1.0
|
CA
|
E:GLY359
|
4.6
|
52.9
|
1.0
|
CZ
|
E:TYR355
|
4.6
|
50.8
|
1.0
|
N
|
E:ILE389
|
4.6
|
47.4
|
1.0
|
CG
|
E:MET356
|
4.6
|
53.2
|
1.0
|
CG
|
E:GLU388
|
4.6
|
48.8
|
1.0
|
CB
|
E:CYS391
|
4.7
|
51.9
|
1.0
|
CA
|
E:ALA390
|
4.7
|
49.0
|
1.0
|
CE2
|
E:TYR355
|
4.7
|
49.9
|
1.0
|
CE
|
E:MET356
|
4.8
|
52.5
|
1.0
|
O
|
E:ILE389
|
4.8
|
50.2
|
1.0
|
CA
|
E:MET356
|
4.9
|
51.4
|
1.0
|
CB
|
E:MET356
|
4.9
|
51.6
|
1.0
|
CA
|
E:ILE389
|
5.0
|
48.4
|
1.0
|
|
Sodium binding site 3 out
of 3 in 2wp9
Go back to
Sodium Binding Sites List in 2wp9
Sodium binding site 3 out
of 3 in the Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant
 Mono view
 Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhb HIS207THR Mutant within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Na1590
b:49.9
occ:1.00
|
O
|
I:ALA390
|
2.5
|
58.3
|
1.0
|
O
|
I:GLU388
|
2.5
|
57.0
|
1.0
|
O
|
I:MET356
|
2.6
|
60.6
|
1.0
|
O
|
I:GLY358
|
2.7
|
61.4
|
1.0
|
O
|
I:MET357
|
3.0
|
62.1
|
1.0
|
C
|
I:MET357
|
3.4
|
62.6
|
1.0
|
C
|
I:GLU388
|
3.5
|
55.9
|
1.0
|
OH
|
I:TYR355
|
3.7
|
64.7
|
1.0
|
C
|
I:ALA390
|
3.7
|
58.4
|
1.0
|
C
|
I:GLY358
|
3.7
|
62.9
|
1.0
|
C
|
I:MET356
|
3.8
|
61.9
|
1.0
|
CA
|
I:GLU388
|
3.9
|
55.1
|
1.0
|
CA
|
I:MET357
|
4.0
|
62.6
|
1.0
|
N
|
I:GLY358
|
4.1
|
63.7
|
1.0
|
O
|
I:GLY387
|
4.2
|
54.5
|
1.0
|
CZ
|
I:TYR355
|
4.3
|
61.5
|
1.0
|
N
|
I:ALA390
|
4.3
|
57.0
|
1.0
|
N
|
I:MET357
|
4.3
|
62.7
|
1.0
|
CG
|
I:GLU388
|
4.4
|
57.9
|
1.0
|
CA
|
I:GLY358
|
4.5
|
63.9
|
1.0
|
CE2
|
I:TYR355
|
4.5
|
61.1
|
1.0
|
CA
|
I:CYS391
|
4.5
|
60.5
|
1.0
|
N
|
I:CYS391
|
4.5
|
59.2
|
1.0
|
N
|
I:ILE389
|
4.6
|
55.3
|
1.0
|
C
|
I:ILE389
|
4.6
|
57.4
|
1.0
|
CA
|
I:ALA390
|
4.7
|
57.6
|
1.0
|
N
|
I:GLY359
|
4.7
|
63.1
|
1.0
|
CB
|
I:GLU388
|
4.8
|
55.2
|
1.0
|
CB
|
I:CYS391
|
4.8
|
61.9
|
1.0
|
CG
|
I:MET356
|
4.8
|
63.0
|
1.0
|
CA
|
I:GLY359
|
4.9
|
62.2
|
1.0
|
CA
|
I:ILE389
|
5.0
|
56.4
|
1.0
|
N
|
I:GLU388
|
5.0
|
54.1
|
1.0
|
CA
|
I:MET356
|
5.0
|
62.0
|
1.0
|
O
|
I:ILE389
|
5.0
|
58.4
|
1.0
|
|
Reference:
J.Ruprecht,
S.Iwata,
R.A.Rothery,
J.H.Weiner,
E.Maklashina,
G.Cecchini.
Perturbation of the Quinone-Binding Site of Complex II Alters the Electronic Properties of the Proximal [3FE-4S] Iron-Sulfur Cluster. J. Biol. Chem. V. 286 12756 2011.
ISSN: ESSN 1083-351X
PubMed: 21310949
DOI: 10.1074/JBC.M110.209874
Page generated: Mon Oct 7 04:51:37 2024
|