Sodium in PDB 2gws: Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
All present enzymatic activity of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus:
2.7.7.7;
Protein crystallography data
The structure of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus, PDB code: 2gws
was solved by
M.Garcia-Diaz,
A.J.Picher,
K.Bebenek,
L.C.Pedersen,
T.A.Kunkel,
L.Blanco,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
50.00 /
2.40
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
192.374,
98.268,
104.960,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
20.9 /
25.3
|
Other elements in 2gws:
The structure of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus also contains other interesting chemical elements:
Sodium Binding Sites:
The binding sites of Sodium atom in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
(pdb code 2gws). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 8 binding sites of Sodium where determined in the
Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus, PDB code: 2gws:
Jump to Sodium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Sodium binding site 1 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 1 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
T:Na944
b:43.1
occ:1.00
|
O
|
T:HOH635
|
2.1
|
44.3
|
1.0
|
O
|
T:HOH636
|
2.2
|
39.1
|
1.0
|
O
|
T:HOH633
|
2.2
|
38.6
|
1.0
|
O
|
D:HOH634
|
2.3
|
44.9
|
1.0
|
O
|
T:HOH631
|
2.3
|
29.7
|
1.0
|
O
|
T:HOH632
|
2.7
|
35.9
|
1.0
|
O6
|
D:DG1
|
3.9
|
34.8
|
1.0
|
O6
|
T:DG3
|
4.0
|
24.9
|
1.0
|
N4
|
D:DC2
|
4.2
|
24.2
|
1.0
|
N7
|
T:DG2
|
4.5
|
35.0
|
1.0
|
O6
|
T:DG2
|
4.5
|
28.4
|
1.0
|
O
|
T:HOH124
|
4.7
|
28.1
|
1.0
|
C6
|
D:DG1
|
4.9
|
30.0
|
1.0
|
|
Sodium binding site 2 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 2 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Na945
b:39.4
occ:1.00
|
O
|
T:HOH507
|
2.2
|
46.4
|
1.0
|
O
|
T:HOH568
|
2.4
|
49.3
|
1.0
|
O6
|
P:DG6
|
2.5
|
27.1
|
1.0
|
O
|
P:HOH282
|
2.5
|
37.0
|
1.0
|
O6
|
T:DG6
|
2.7
|
26.2
|
1.0
|
O6
|
P:DG5
|
2.8
|
17.6
|
1.0
|
C6
|
P:DG6
|
3.5
|
25.8
|
1.0
|
C6
|
P:DG5
|
3.6
|
18.5
|
1.0
|
C6
|
T:DG6
|
3.7
|
22.2
|
1.0
|
N1
|
P:DG6
|
3.8
|
25.1
|
1.0
|
O
|
T:HOH434
|
3.9
|
32.4
|
1.0
|
N1
|
T:DG6
|
4.0
|
22.6
|
1.0
|
N7
|
P:DG5
|
4.1
|
19.8
|
1.0
|
C5
|
P:DG5
|
4.1
|
19.5
|
1.0
|
O
|
T:HOH455
|
4.1
|
33.5
|
1.0
|
O
|
P:HOH506
|
4.2
|
45.6
|
1.0
|
N4
|
T:DC7
|
4.2
|
21.7
|
1.0
|
N1
|
P:DG5
|
4.6
|
15.3
|
1.0
|
C5
|
P:DG6
|
4.8
|
23.3
|
1.0
|
O
|
P:HOH144
|
4.9
|
43.5
|
1.0
|
C5
|
T:DG6
|
5.0
|
23.8
|
1.0
|
|
Sodium binding site 3 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 3 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
Q:Na948
b:53.3
occ:1.00
|
O
|
Q:HOH805
|
2.4
|
47.5
|
1.0
|
O
|
N:HOH2834
|
2.5
|
40.7
|
1.0
|
O
|
N:HOH2835
|
2.5
|
52.0
|
1.0
|
O
|
Q:HOH416
|
3.1
|
37.2
|
1.0
|
O6
|
Q:DG1
|
3.8
|
29.4
|
1.0
|
N4
|
Q:DC2
|
4.0
|
29.2
|
1.0
|
O6
|
N:DG3
|
4.1
|
27.8
|
1.0
|
O6
|
N:DG2
|
4.6
|
38.1
|
1.0
|
O
|
N:HOH2811
|
4.6
|
41.7
|
1.0
|
C6
|
Q:DG1
|
4.6
|
26.7
|
1.0
|
N7
|
Q:DG1
|
4.7
|
30.3
|
1.0
|
O
|
Q:HOH435
|
4.7
|
38.8
|
1.0
|
|
Sodium binding site 4 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 4 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 4 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na940
b:25.5
occ:1.00
|
O
|
A:SER339
|
2.3
|
28.7
|
1.0
|
O
|
P:HOH423
|
2.4
|
27.8
|
1.0
|
OP1
|
P:DG5
|
2.4
|
19.0
|
1.0
|
O
|
A:ALA344
|
2.5
|
25.9
|
1.0
|
O
|
A:ILE341
|
2.7
|
26.1
|
1.0
|
O
|
A:HOH959
|
2.8
|
33.4
|
1.0
|
C
|
A:SER339
|
3.4
|
30.1
|
1.0
|
O
|
A:HOH1074
|
3.4
|
43.6
|
1.0
|
C
|
A:ALA344
|
3.5
|
23.8
|
1.0
|
P
|
P:DG5
|
3.5
|
23.9
|
1.0
|
C
|
A:ILE341
|
3.7
|
28.4
|
1.0
|
OP2
|
P:DG5
|
3.7
|
20.7
|
1.0
|
N
|
A:ALA344
|
3.8
|
24.4
|
1.0
|
CA
|
A:SER339
|
4.0
|
32.0
|
1.0
|
N
|
A:GLY343
|
4.2
|
25.5
|
1.0
|
N
|
A:ILE341
|
4.2
|
26.6
|
1.0
|
CA
|
A:ALA344
|
4.2
|
24.7
|
1.0
|
CA
|
A:TRP342
|
4.4
|
26.8
|
1.0
|
N
|
A:TRP342
|
4.4
|
27.7
|
1.0
|
CB
|
A:SER339
|
4.4
|
29.9
|
1.0
|
N
|
A:GLY345
|
4.4
|
25.2
|
1.0
|
N
|
A:ASN340
|
4.4
|
28.2
|
1.0
|
C
|
A:ASN340
|
4.4
|
27.9
|
1.0
|
O3'
|
P:DC4
|
4.5
|
21.6
|
1.0
|
CA
|
A:ILE341
|
4.6
|
27.2
|
1.0
|
CA
|
A:GLY345
|
4.6
|
24.7
|
1.0
|
C
|
A:TRP342
|
4.6
|
26.4
|
1.0
|
O5'
|
P:DG5
|
4.7
|
21.7
|
1.0
|
CA
|
A:ASN340
|
4.7
|
27.9
|
1.0
|
CB
|
A:ALA344
|
4.8
|
22.8
|
1.0
|
C
|
A:GLY343
|
4.8
|
25.0
|
1.0
|
O
|
P:HOH273
|
4.9
|
35.0
|
1.0
|
O
|
A:ASN340
|
5.0
|
29.1
|
1.0
|
|
Sodium binding site 5 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 5 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 5 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
E:Na941
b:44.8
occ:1.00
|
O
|
E:SER339
|
2.3
|
54.6
|
1.0
|
OP1
|
G:DG5
|
2.4
|
48.6
|
1.0
|
O
|
E:ILE341
|
2.5
|
51.1
|
1.0
|
O
|
E:ALA344
|
2.5
|
41.1
|
1.0
|
O
|
E:HOH2821
|
3.0
|
47.6
|
1.0
|
O
|
G:HOH262
|
3.0
|
36.2
|
1.0
|
C
|
E:SER339
|
3.3
|
55.1
|
1.0
|
C
|
E:ILE341
|
3.3
|
52.7
|
1.0
|
C
|
E:ALA344
|
3.4
|
42.9
|
1.0
|
N
|
E:ALA344
|
3.6
|
45.1
|
1.0
|
P
|
G:DG5
|
3.6
|
48.4
|
1.0
|
N
|
E:ILE341
|
3.9
|
52.9
|
1.0
|
N
|
E:GLY343
|
3.9
|
49.1
|
1.0
|
CA
|
E:SER339
|
3.9
|
55.6
|
1.0
|
OP2
|
G:DG5
|
3.9
|
46.6
|
1.0
|
N
|
E:TRP342
|
4.0
|
51.4
|
1.0
|
CA
|
E:ALA344
|
4.0
|
44.1
|
1.0
|
CA
|
E:TRP342
|
4.0
|
51.5
|
1.0
|
C
|
E:ASN340
|
4.1
|
54.5
|
1.0
|
N
|
E:ASN340
|
4.2
|
54.5
|
1.0
|
CA
|
E:ILE341
|
4.2
|
52.6
|
1.0
|
C
|
E:TRP342
|
4.3
|
50.7
|
1.0
|
CB
|
E:ALA344
|
4.4
|
43.0
|
1.0
|
N
|
E:GLY345
|
4.4
|
43.1
|
1.0
|
CA
|
E:ASN340
|
4.5
|
54.4
|
1.0
|
CB
|
E:SER339
|
4.5
|
54.5
|
1.0
|
C
|
E:GLY343
|
4.6
|
46.3
|
1.0
|
O3'
|
G:DC4
|
4.6
|
47.0
|
1.0
|
O
|
E:ASN340
|
4.7
|
56.2
|
1.0
|
O5'
|
G:DG5
|
4.7
|
46.8
|
1.0
|
CA
|
E:GLY345
|
4.8
|
43.9
|
1.0
|
CA
|
E:GLY343
|
4.8
|
47.0
|
1.0
|
O
|
E:PHE338
|
4.9
|
54.2
|
1.0
|
|
Sodium binding site 6 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 6 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 6 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Na942
b:41.2
occ:1.00
|
O
|
I:SER339
|
2.2
|
42.5
|
1.0
|
O
|
I:ILE341
|
2.4
|
39.5
|
1.0
|
OP1
|
K:DG5
|
2.5
|
38.1
|
1.0
|
O
|
I:ALA344
|
2.5
|
39.5
|
1.0
|
O
|
K:HOH648
|
2.6
|
36.9
|
1.0
|
O
|
I:HOH989
|
3.3
|
50.4
|
1.0
|
C
|
I:SER339
|
3.3
|
43.2
|
1.0
|
C
|
I:ILE341
|
3.3
|
39.1
|
1.0
|
C
|
I:ALA344
|
3.6
|
40.2
|
1.0
|
P
|
K:DG5
|
3.7
|
38.0
|
1.0
|
N
|
I:ALA344
|
3.9
|
38.7
|
1.0
|
N
|
I:ILE341
|
3.9
|
38.9
|
1.0
|
CA
|
I:SER339
|
4.0
|
45.3
|
1.0
|
OP2
|
K:DG5
|
4.0
|
38.2
|
1.0
|
CA
|
I:TRP342
|
4.0
|
39.1
|
1.0
|
N
|
I:TRP342
|
4.0
|
38.9
|
1.0
|
N
|
I:GLY343
|
4.0
|
38.2
|
1.0
|
C
|
I:ASN340
|
4.2
|
40.3
|
1.0
|
CA
|
I:ALA344
|
4.3
|
39.9
|
1.0
|
CA
|
I:ILE341
|
4.3
|
38.3
|
1.0
|
N
|
I:ASN340
|
4.3
|
40.4
|
1.0
|
C
|
I:TRP342
|
4.4
|
39.7
|
1.0
|
CA
|
I:ASN340
|
4.5
|
40.6
|
1.0
|
CB
|
I:ALA344
|
4.7
|
39.9
|
1.0
|
O
|
I:ASN340
|
4.7
|
42.1
|
1.0
|
N
|
I:GLY345
|
4.7
|
41.1
|
1.0
|
CB
|
I:SER339
|
4.7
|
46.2
|
1.0
|
O5'
|
K:DG5
|
4.7
|
38.1
|
1.0
|
O3'
|
K:DC4
|
4.8
|
38.4
|
1.0
|
O
|
I:PHE338
|
4.8
|
43.1
|
1.0
|
C
|
I:GLY343
|
4.8
|
39.5
|
1.0
|
CA
|
I:GLY345
|
4.9
|
40.0
|
1.0
|
|
Sodium binding site 7 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 7 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 7 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
M:Na943
b:25.0
occ:1.00
|
OP1
|
R:DG5
|
2.3
|
17.9
|
1.0
|
O
|
M:SER339
|
2.4
|
23.3
|
1.0
|
O
|
M:ALA344
|
2.4
|
24.3
|
1.0
|
O
|
R:HOH33
|
2.4
|
20.3
|
1.0
|
O
|
M:ILE341
|
2.6
|
22.0
|
1.0
|
O
|
M:HOH1033
|
2.7
|
29.1
|
1.0
|
C
|
M:ALA344
|
3.4
|
22.9
|
1.0
|
C
|
M:SER339
|
3.5
|
24.6
|
1.0
|
P
|
R:DG5
|
3.5
|
21.6
|
1.0
|
C
|
M:ILE341
|
3.5
|
21.2
|
1.0
|
O
|
M:HOH1046
|
3.6
|
32.8
|
1.0
|
N
|
M:ALA344
|
3.6
|
22.0
|
1.0
|
OP2
|
R:DG5
|
3.8
|
17.3
|
1.0
|
N
|
M:GLY343
|
3.9
|
21.3
|
1.0
|
CA
|
M:TRP342
|
4.0
|
21.3
|
1.0
|
CA
|
M:ALA344
|
4.1
|
22.6
|
1.0
|
N
|
M:TRP342
|
4.1
|
20.7
|
1.0
|
N
|
M:ILE341
|
4.1
|
20.7
|
1.0
|
CA
|
M:SER339
|
4.2
|
25.3
|
1.0
|
O
|
M:HOH1183
|
4.2
|
53.1
|
1.0
|
C
|
M:TRP342
|
4.3
|
21.6
|
1.0
|
C
|
M:ASN340
|
4.3
|
24.7
|
1.0
|
N
|
M:ASN340
|
4.5
|
25.0
|
1.0
|
N
|
M:GLY345
|
4.5
|
23.2
|
1.0
|
CA
|
M:ILE341
|
4.5
|
21.6
|
1.0
|
CB
|
M:ALA344
|
4.5
|
21.2
|
1.0
|
O3'
|
R:DC4
|
4.5
|
20.7
|
1.0
|
O5'
|
R:DG5
|
4.5
|
14.7
|
1.0
|
C
|
M:GLY343
|
4.6
|
23.2
|
1.0
|
CA
|
M:ASN340
|
4.6
|
24.8
|
1.0
|
CB
|
M:SER339
|
4.7
|
24.1
|
1.0
|
CA
|
M:GLY345
|
4.7
|
20.0
|
1.0
|
CA
|
M:GLY343
|
4.8
|
21.7
|
1.0
|
O
|
M:ASN340
|
4.8
|
27.2
|
1.0
|
|
Sodium binding site 8 out
of 8 in 2gws
Go back to
Sodium Binding Sites List in 2gws
Sodium binding site 8 out
of 8 in the Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 8 of Crystal Structure of Human Dna Polymerase Lambda with A G/G Mismatch in the Primer Terminus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
M:Na947
b:43.2
occ:1.00
|
O
|
M:HOH1033
|
2.9
|
29.1
|
1.0
|
O
|
M:ASN340
|
3.6
|
27.2
|
1.0
|
O
|
M:HOH997
|
4.3
|
25.5
|
1.0
|
O
|
M:HOH1183
|
4.3
|
53.1
|
1.0
|
C
|
M:ASN340
|
4.4
|
24.7
|
1.0
|
O
|
M:PHE383
|
4.6
|
23.4
|
1.0
|
O
|
M:HOH1046
|
4.8
|
32.8
|
1.0
|
CA
|
M:ASN340
|
4.8
|
24.8
|
1.0
|
CD2
|
M:LEU384
|
4.9
|
29.9
|
1.0
|
N
|
M:TRP342
|
4.9
|
20.7
|
1.0
|
|
Reference:
A.J.Picher,
M.Garcia-Diaz,
K.Bebenek,
L.C.Pedersen,
T.A.Kunkel,
L.Blanco.
Promiscuous Mismatch Extension By Human Dna Polymerase Lambda. Nucleic Acids Res. V. 34 3259 2006.
ISSN: ISSN 0305-1048
PubMed: 16807316
DOI: 10.1093/NAR/GKL377
Page generated: Mon Oct 7 02:43:11 2024
|