Sodium in PDB 2gie: Hincii Bound to Cognate Dna Gttaac

All present enzymatic activity of Hincii Bound to Cognate Dna Gttaac:
3.1.21.4;

The structure of Hincii Bound to Cognate Dna Gttaac, PDB code: 2gie was solved by N.C.Horton, H.K.Joshi, C.Etzkorn, L.Chatwell, J.Bitinaite, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.60
Space group	I 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	67.243, 175.472, 253.896, 90.00, 90.00, 90.00
R / Rfree (%)	23.8 / 30.6

The binding sites of Sodium atom in the Hincii Bound to Cognate Dna Gttaac (pdb code 2gie). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total only one binding site of Sodium was determined in the Hincii Bound to Cognate Dna Gttaac, PDB code: 2gie:

Sodium binding site 1 out of 1 in the Hincii Bound to Cognate Dna Gttaac


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Hincii Bound to Cognate Dna Gttaac within 5.0Å range: probe atom residue distance (Å) B Occ B:Na601 b:41.5 occ:1.00 OP1 E:DA8 2.3 26.9 1.0 OD1 B:ASP114 2.4 33.7 1.0 O B:HOH703 2.5 35.5 1.0 O B:VAL128 2.8 30.3 1.0 O E:HOH430 2.8 48.8 1.0 OD1 B:ASP127 3.0 53.0 1.0 OD2 B:ASP114 3.0 39.1 1.0 CG B:ASP114 3.1 35.5 1.0 P E:DA8 3.4 29.5 1.0 O E:HOH188 3.6 52.8 1.0 O5' E:DA8 3.7 27.1 1.0 C B:VAL128 3.8 30.4 1.0 N B:VAL128 3.9 33.8 1.0 C5' E:DA8 3.9 24.0 1.0 O E:HOH429 4.0 42.2 1.0 O E:HOH508 4.1 49.5 1.0 CG B:ASP127 4.1 50.4 1.0 OP2 E:DA8 4.2 25.8 1.0 CA B:VAL128 4.3 30.5 1.0 OP2 E:DA9 4.5 22.0 1.0 CB B:ASP114 4.5 34.8 1.0 C3' E:DA8 4.5 25.8 1.0 NZ B:LYS129 4.7 33.9 1.0 CB B:VAL128 4.7 31.1 1.0 C4' E:DA8 4.7 23.9 1.0 OD2 B:ASP127 4.7 52.4 1.0 O3' E:DT7 4.7 26.9 1.0 O3' E:DA8 4.8 24.5 1.0 C B:ASP127 4.8 36.7 1.0 N B:LYS129 4.9 29.4 1.0

H.K.Joshi, C.Etzkorn, L.Chatwell, J.Bitinaite, N.C.Horton. Alteration of Sequence Specificity of the Type II Restriction Endonuclease Hincii Through An Indirect Readout Mechanism. J.Biol.Chem. V. 281 23852 2006.
ISSN: ISSN 0021-9258
PubMed: 16675462
DOI: 10.1074/JBC.M512339200