Sodium in PDB 1ud8: Crystal Structure of AMYK38 with Lithium Ion
Enzymatic activity of Crystal Structure of AMYK38 with Lithium Ion
All present enzymatic activity of Crystal Structure of AMYK38 with Lithium Ion:
3.2.1.1;
Protein crystallography data
The structure of Crystal Structure of AMYK38 with Lithium Ion, PDB code: 1ud8
was solved by
T.Nonaka,
M.Fujihashi,
A.Kita,
H.Hagihara,
K.Ozaki,
S.Ito,
K.Miki,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
59.28 /
2.88
|
Space group
|
P 2 3
|
Cell size a, b, c (Å), α, β, γ (°)
|
132.553,
132.553,
132.553,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
20.5 /
26.8
|
Sodium Binding Sites:
The binding sites of Sodium atom in the Crystal Structure of AMYK38 with Lithium Ion
(pdb code 1ud8). This binding sites where shown within
5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the
Crystal Structure of AMYK38 with Lithium Ion, PDB code: 1ud8:
Jump to Sodium binding site number:
1;
2;
3;
Sodium binding site 1 out
of 3 in 1ud8
Go back to
Sodium Binding Sites List in 1ud8
Sodium binding site 1 out
of 3 in the Crystal Structure of AMYK38 with Lithium Ion
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 1 of Crystal Structure of AMYK38 with Lithium Ion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1001
b:21.1
occ:1.00
|
O
|
A:HIS235
|
2.3
|
32.3
|
1.0
|
OD1
|
A:ASN104
|
2.4
|
28.5
|
1.0
|
OD1
|
A:ASP194
|
2.4
|
23.9
|
1.0
|
O
|
A:ASP194
|
2.5
|
25.5
|
1.0
|
OD1
|
A:ASN200
|
2.6
|
38.3
|
1.0
|
C
|
A:HIS235
|
3.3
|
28.4
|
1.0
|
CG
|
A:ASN200
|
3.4
|
35.4
|
1.0
|
ND2
|
A:ASN200
|
3.4
|
36.6
|
1.0
|
CG
|
A:ASN104
|
3.5
|
25.6
|
1.0
|
C
|
A:ASP194
|
3.5
|
26.5
|
1.0
|
CG
|
A:ASP194
|
3.6
|
26.7
|
1.0
|
CB
|
A:HIS235
|
3.8
|
29.4
|
1.0
|
CA
|
A:ASP194
|
4.0
|
28.4
|
1.0
|
O
|
A:ASN104
|
4.0
|
26.4
|
1.0
|
ND2
|
A:ASN104
|
4.1
|
26.4
|
1.0
|
N
|
A:ILE236
|
4.1
|
27.1
|
1.0
|
CA
|
A:HIS235
|
4.2
|
27.5
|
1.0
|
ND2
|
A:ASN183
|
4.2
|
26.8
|
1.0
|
CA
|
A:ILE236
|
4.3
|
26.4
|
1.0
|
CB
|
A:ASP194
|
4.4
|
26.4
|
1.0
|
OD2
|
A:ASP194
|
4.4
|
26.9
|
1.0
|
O
|
A:HOH2086
|
4.4
|
44.8
|
1.0
|
CB
|
A:ASN104
|
4.6
|
24.9
|
1.0
|
N
|
A:TYR195
|
4.7
|
25.2
|
1.0
|
O
|
A:ILE201
|
4.7
|
34.4
|
1.0
|
CG1
|
A:ILE236
|
4.8
|
22.3
|
1.0
|
CA
|
A:ASN104
|
4.8
|
25.3
|
1.0
|
C
|
A:ASN104
|
4.8
|
24.7
|
1.0
|
CB
|
A:ASN200
|
4.8
|
31.9
|
1.0
|
CG
|
A:ASN183
|
5.0
|
28.7
|
1.0
|
CD
|
A:PRO237
|
5.0
|
27.2
|
1.0
|
|
Sodium binding site 2 out
of 3 in 1ud8
Go back to
Sodium Binding Sites List in 1ud8
Sodium binding site 2 out
of 3 in the Crystal Structure of AMYK38 with Lithium Ion
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 2 of Crystal Structure of AMYK38 with Lithium Ion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1002
b:37.8
occ:1.00
|
O
|
A:GLY300
|
2.2
|
25.0
|
1.0
|
OD1
|
A:ASP404
|
2.3
|
22.4
|
1.0
|
O
|
A:TYR302
|
2.4
|
29.9
|
1.0
|
O
|
A:TRP403
|
2.5
|
30.5
|
1.0
|
O
|
A:HOH2018
|
2.5
|
14.2
|
1.0
|
OD1
|
A:ASN427
|
3.1
|
32.5
|
1.0
|
ND2
|
A:ASN427
|
3.3
|
27.4
|
1.0
|
C
|
A:GLY300
|
3.4
|
26.6
|
1.0
|
C
|
A:TRP403
|
3.4
|
30.2
|
1.0
|
CG
|
A:ASP404
|
3.5
|
19.0
|
1.0
|
CG
|
A:ASN427
|
3.6
|
31.2
|
1.0
|
C
|
A:TYR302
|
3.6
|
27.2
|
1.0
|
CA
|
A:ASP404
|
3.8
|
24.4
|
1.0
|
N
|
A:ASP404
|
4.0
|
26.4
|
1.0
|
C
|
A:SER301
|
4.1
|
25.4
|
1.0
|
N
|
A:MET304
|
4.2
|
24.4
|
1.0
|
CA
|
A:GLY300
|
4.2
|
27.5
|
1.0
|
CB
|
A:ASP404
|
4.2
|
20.2
|
1.0
|
O
|
A:SER301
|
4.2
|
27.1
|
1.0
|
CA
|
A:ASP303
|
4.2
|
26.7
|
1.0
|
N
|
A:TYR302
|
4.3
|
24.7
|
1.0
|
N
|
A:ASP303
|
4.3
|
27.6
|
1.0
|
CD1
|
A:TRP403
|
4.4
|
43.3
|
1.0
|
N
|
A:SER301
|
4.4
|
25.0
|
1.0
|
CB
|
A:TRP403
|
4.5
|
32.5
|
1.0
|
OD2
|
A:ASP404
|
4.5
|
18.0
|
1.0
|
CA
|
A:TRP403
|
4.5
|
30.9
|
1.0
|
CA
|
A:TYR302
|
4.6
|
25.1
|
1.0
|
CA
|
A:SER301
|
4.6
|
24.4
|
1.0
|
CG
|
A:MET304
|
4.7
|
25.6
|
1.0
|
C
|
A:ASP303
|
4.8
|
25.4
|
1.0
|
CG
|
A:TRP403
|
4.8
|
39.7
|
1.0
|
|
Sodium binding site 3 out
of 3 in 1ud8
Go back to
Sodium Binding Sites List in 1ud8
Sodium binding site 3 out
of 3 in the Crystal Structure of AMYK38 with Lithium Ion
Mono view
Stereo pair view
|
A full contact list of Sodium with other atoms in the Na binding
site number 3 of Crystal Structure of AMYK38 with Lithium Ion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Na1003
b:31.3
occ:0.92
|
OD1
|
A:ASN289
|
2.7
|
29.3
|
1.0
|
O
|
A:SER337
|
2.7
|
28.6
|
1.0
|
O
|
A:ASN289
|
2.8
|
25.8
|
1.0
|
OD1
|
A:ASP325
|
2.8
|
20.4
|
1.0
|
O
|
A:VAL324
|
3.0
|
9.4
|
1.0
|
CG
|
A:ASN289
|
3.0
|
28.2
|
1.0
|
ND2
|
A:ASN289
|
3.2
|
24.4
|
1.0
|
CG2
|
A:VAL339
|
3.5
|
12.3
|
1.0
|
C
|
A:ASN289
|
3.6
|
25.9
|
1.0
|
CG
|
A:ASP325
|
3.7
|
21.1
|
1.0
|
C
|
A:SER337
|
3.9
|
30.1
|
1.0
|
CA
|
A:ASN289
|
4.0
|
25.3
|
1.0
|
CB
|
A:TYR293
|
4.0
|
20.8
|
1.0
|
CB
|
A:ASN289
|
4.1
|
26.3
|
1.0
|
OG
|
A:SER337
|
4.1
|
34.1
|
1.0
|
OD2
|
A:ASP325
|
4.1
|
22.6
|
1.0
|
C
|
A:VAL324
|
4.2
|
13.1
|
1.0
|
CB
|
A:SER337
|
4.2
|
32.4
|
1.0
|
N
|
A:TYR293
|
4.3
|
21.6
|
1.0
|
CA
|
A:TYR293
|
4.5
|
21.2
|
1.0
|
N
|
A:VAL339
|
4.5
|
21.0
|
1.0
|
CB
|
A:PHE292
|
4.7
|
13.3
|
1.0
|
N
|
A:TYR290
|
4.7
|
24.6
|
1.0
|
CA
|
A:SER337
|
4.7
|
33.3
|
1.0
|
CB
|
A:VAL339
|
4.8
|
18.8
|
1.0
|
N
|
A:TRP338
|
4.8
|
26.7
|
1.0
|
CG
|
A:TYR293
|
4.9
|
21.9
|
1.0
|
CD1
|
A:TYR293
|
4.9
|
20.6
|
1.0
|
CA
|
A:ASP325
|
4.9
|
18.9
|
1.0
|
C
|
A:TRP338
|
4.9
|
23.3
|
1.0
|
CB
|
A:ASP325
|
4.9
|
17.1
|
1.0
|
CA
|
A:TRP338
|
5.0
|
22.5
|
1.0
|
|
Reference:
T.Nonaka,
M.Fujihashi,
A.Kita,
H.Hagihara,
K.Ozaki,
S.Ito,
K.Miki.
Crystal Structure of Calcium-Free Alpha-Amylase From Bacillus Sp. Strain Ksm-K38 (AMYK38) and Its Sodium Ion Binding Sites J.Biol.Chem. V. 278 24818 2003.
ISSN: ISSN 0021-9258
PubMed: 12719434
DOI: 10.1074/JBC.M212763200
Page generated: Sun Oct 6 22:45:03 2024
|