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Sodium in PDB 1spb: Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C

Enzymatic activity of Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C

All present enzymatic activity of Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C:
3.4.21.62;

Protein crystallography data

The structure of Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C, PDB code: 1spb was solved by D.T.Gallagher, G.L.Gilliland, L.Wang, P.N.Bryan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 2.00
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 74.100, 77.850, 57.650, 90.00, 90.00, 90.00
R / Rfree (%) n/a / n/a

Sodium Binding Sites:

The binding sites of Sodium atom in the Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C (pdb code 1spb). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total only one binding site of Sodium was determined in the Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C, PDB code: 1spb:

Sodium binding site 1 out of 1 in 1spb

Go back to Sodium Binding Sites List in 1spb
Sodium binding site 1 out of 1 in the Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Subtilisin Bpn' Prosegment (77 Residues) Complexed with A Mutant Subtilisin Bpn' (266 Residues). Crystal pH 4.6. Crystallization Temperature 20 C Diffraction Temperature- 160 C within 5.0Å range:
probe atom residue distance (Å) B Occ
S:Na297

b:11.2
occ:0.92
 O S:GLY169 2.5 8.2 1.0
O S:TYR171 2.6 8.6 1.0
O S:VAL174 2.6 2.0 1.0
O S:HOH304 2.8 3.9 1.0
OD2 S:ASP197 3.0 5.5 1.0
O S:GLU195 3.3 2.9 1.0
O S:HOH390 3.4 13.0 0.9
C S:VAL174 3.6 2.5 1.0
C S:TYR171 3.6 9.7 1.0
C S:GLY169 3.7 9.4 1.0
CB S:ALA176 3.9 5.5 1.0
N S:ALA176 4.0 6.1 1.0
CG S:ASP197 4.1 4.8 1.0
C S:LYS170 4.2 9.9 1.0
N S:TYR171 4.2 11.2 1.0
O S:LYS170 4.3 10.4 1.0
C S:GLU195 4.3 4.4 1.0
NH2 S:ARG247 4.3 4.2 1.0
CA S:VAL174 4.4 3.4 1.0
OD1 S:ASP197 4.4 3.6 1.0
CB S:GLU195 4.4 4.9 1.0
N S:PRO172 4.5 9.3 1.0
CA S:TYR171 4.5 10.7 1.0
N S:LYS170 4.5 9.5 1.0
CA S:ALA176 4.5 5.5 1.0
N S:VAL174 4.5 5.8 1.0
CB S:VAL174 4.5 3.9 1.0
CA S:LYS170 4.6 10.0 1.0
CA S:PRO172 4.6 9.6 1.0
N S:ILE175 4.6 2.5 1.0
CA S:GLU195 4.6 4.9 1.0
O S:PRO172 4.7 6.5 1.0
CA S:GLY169 4.7 8.2 1.0
C S:PRO172 4.8 7.5 1.0
C S:ILE175 4.8 4.2 1.0
CA S:ILE175 4.8 4.6 1.0
O S:HOH500 5.0 23.4 1.0

Reference:

T.Gallagher, G.Gilliland, L.Wang, P.Bryan. The Prosegment-Subtilisin Bpn' Complex: Crystal Structure of A Specific 'Foldase'. Structure V. 3 907 1995.
ISSN: ISSN 0969-2126
PubMed: 8535784
DOI: 10.1016/S0969-2126(01)00225-8
Page generated: Tue Dec 15 05:36:04 2020

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