Sodium in PDB 1sa3: An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site

All present enzymatic activity of An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site:
3.1.21.4;

The structure of An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site, PDB code: 1sa3 was solved by Q.S.Xu, R.B.Kucera, R.J.Roberts, H.C.Guo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.58 / 1.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.193, 131.601, 59.318, 90.00, 109.69, 90.00
R / Rfree (%)	22.3 / 25.2

The binding sites of Sodium atom in the An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site (pdb code 1sa3). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site, PDB code: 1sa3:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Na401 b:29.8 occ:1.00 O A:HOH573 2.3 31.6 1.0 OE1 A:GLU35 2.3 24.0 1.0 OD2 A:ASP84 2.4 23.7 1.0 O A:HOH546 2.4 40.5 1.0 O A:ASP99 2.7 16.3 1.0 OE2 A:GLU35 2.8 23.0 1.0 CD A:GLU35 2.9 20.9 1.0 CG A:ASP84 3.5 24.7 1.0 C A:ASP99 3.6 16.2 1.0 O A:HOH570 3.8 38.8 1.0 CA A:ASP99 3.9 15.2 1.0 CB A:ASP99 4.0 14.6 1.0 OD1 A:ASP84 4.2 28.6 1.0 CG A:GLU35 4.3 17.5 1.0 O A:HOH551 4.3 36.7 1.0 O A:THR85 4.4 23.2 1.0 OD1 A:ASP99 4.4 19.1 1.0 N A:THR85 4.5 17.6 1.0 CE A:LYS32 4.6 23.5 0.0 CB A:ASP84 4.6 18.5 1.0 CG A:ASP99 4.6 14.8 1.0 O A:HOH566 4.7 32.6 1.0 N A:ALA100 4.8 14.0 1.0 CA A:ASP84 4.8 18.2 1.0

Sodium binding site 2 out of 2 in the An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site within 5.0Å range: probe atom residue distance (Å) B Occ B:Na402 b:28.2 occ:1.00 OD2 B:ASP84 2.3 32.3 1.0 O B:HOH419 2.3 26.7 1.0 O B:HOH448 2.4 35.2 1.0 OE1 B:GLU35 2.4 36.1 1.0 O B:ASP99 2.5 18.4 1.0 OE2 B:GLU35 2.8 28.7 1.0 CD B:GLU35 2.9 31.7 1.0 C B:ASP99 3.4 16.2 1.0 CG B:ASP84 3.5 31.1 1.0 O B:HOH441 3.6 41.1 1.0 CA B:ASP99 3.7 16.9 1.0 CB B:ASP99 3.8 17.3 1.0 OD1 B:ASP84 4.2 33.8 1.0 O B:THR85 4.3 18.7 1.0 N B:THR85 4.4 22.4 1.0 CG B:GLU35 4.4 31.4 1.0 OD1 B:ASP99 4.5 24.5 1.0 CB B:ASP84 4.5 29.0 1.0 CG B:ASP99 4.6 20.9 1.0 N B:ALA100 4.7 16.7 1.0 CA B:ASP84 4.7 26.0 1.0

Q.S.Xu, R.B.Kucera, R.J.Roberts, H.C.Guo. An Asymmetric Complex of Restriction Endonuclease Mspi on Its Palindromic Dna Recognition Site. Structure V. 12 1741 2004.
ISSN: ISSN 0969-2126
PubMed: 15341737
DOI: 10.1016/J.STR.2004.07.014