Sodium in PDB 1m64: Crystal Structure of Q363F Mutant Flavocytochrome C3

All present enzymatic activity of Crystal Structure of Q363F Mutant Flavocytochrome C3:
1.3.99.1;

The structure of Crystal Structure of Q363F Mutant Flavocytochrome C3, PDB code: 1m64 was solved by C.G.Mowat, K.L.Pankhurst, C.S.Miles, D.Leys, M.D.Walkinshaw, G.A.Reid, S.K.Chapman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	78.520, 88.886, 91.194, 90.00, 104.42, 90.00
R / Rfree (%)	16.3 / 22.4

The structure of Crystal Structure of Q363F Mutant Flavocytochrome C3 also contains other interesting chemical elements:

Iron

(Fe)

8 atoms

The binding sites of Sodium atom in the Crystal Structure of Q363F Mutant Flavocytochrome C3 (pdb code 1m64). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Q363F Mutant Flavocytochrome C3, PDB code: 1m64:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Q363F Mutant Flavocytochrome C3


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Q363F Mutant Flavocytochrome C3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Na3002 b:9.8 occ:1.00 O A:HOH3009 2.5 9.5 1.0 O A:THR536 2.5 8.7 1.0 O A:GLU534 2.5 7.3 1.0 O A:THR506 2.5 6.7 1.0 O A:GLY508 2.6 7.6 1.0 O A:MET507 3.1 9.7 1.0 C A:MET507 3.3 10.1 1.0 C A:GLY508 3.5 9.3 1.0 C A:GLU534 3.5 8.3 1.0 O A:HOH3019 3.6 10.4 1.0 C A:THR506 3.7 9.0 1.0 C A:THR536 3.7 10.3 1.0 CA A:MET507 3.7 9.1 1.0 N A:GLY508 3.7 9.4 1.0 CA A:GLU534 4.0 7.3 1.0 CG A:GLU534 4.0 7.5 1.0 O A:HOH3033 4.1 9.9 1.0 CA A:GLY508 4.1 9.7 1.0 N A:MET507 4.1 9.1 1.0 N A:THR536 4.2 9.6 1.0 O A:HOH3054 4.4 11.8 1.0 C A:VAL535 4.4 9.7 1.0 N A:GLY509 4.5 8.6 1.0 N A:GLY537 4.5 9.2 1.0 CA A:THR536 4.5 9.5 1.0 N A:VAL535 4.5 8.8 1.0 CA A:GLY537 4.6 11.0 1.0 CB A:GLU534 4.6 7.9 1.0 NE2 A:HIS505 4.7 9.1 1.0 CA A:GLY509 4.7 8.9 1.0 CA A:VAL535 4.8 9.5 1.0 O A:VAL535 4.8 8.6 1.0 OG1 A:THR506 4.8 6.2 1.0 O A:GLY533 4.8 8.0 1.0 CA A:THR506 4.9 7.6 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Q363F Mutant Flavocytochrome C3


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Q363F Mutant Flavocytochrome C3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Na4002 b:10.1 occ:1.00 O B:HOH4016 2.5 8.7 1.0 O B:GLU534 2.5 7.6 1.0 O B:THR506 2.5 7.9 1.0 O B:THR536 2.5 9.1 1.0 O B:GLY508 2.6 8.4 1.0 O B:MET507 3.1 8.9 1.0 C B:MET507 3.3 9.5 1.0 C B:GLU534 3.5 7.7 1.0 C B:GLY508 3.5 11.0 1.0 O B:HOH4034 3.5 10.1 1.0 C B:THR536 3.6 10.3 1.0 C B:THR506 3.7 7.1 1.0 CA B:MET507 3.7 7.7 1.0 N B:GLY508 3.7 9.6 1.0 CA B:GLU534 4.0 7.5 1.0 CG B:GLU534 4.0 7.0 1.0 O B:HOH4060 4.1 9.4 1.0 CA B:GLY508 4.1 9.6 1.0 N B:MET507 4.1 7.3 1.0 N B:THR536 4.2 8.0 1.0 O B:HOH4041 4.3 11.0 1.0 C B:VAL535 4.4 7.0 1.0 N B:GLY509 4.5 8.9 1.0 N B:GLY537 4.5 9.6 1.0 CA B:THR536 4.5 7.8 1.0 N B:VAL535 4.5 8.1 1.0 CA B:GLY537 4.6 9.8 1.0 CB B:GLU534 4.6 6.8 1.0 CA B:GLY509 4.7 10.3 1.0 NE2 B:HIS505 4.8 8.4 1.0 O B:GLY533 4.8 7.5 1.0 OG1 B:THR506 4.8 6.7 1.0 CA B:VAL535 4.8 6.9 1.0 O B:VAL535 4.8 7.0 1.0 CA B:THR506 4.9 8.1 1.0

C.G.Mowat, K.L.Pankhurst, C.S.Miles, D.Leys, M.D.Walkinshaw, G.A.Reid, S.K.Chapman. Engineering Water to Act As An Active Site Acid Catalyst in A Soluble Fumarate Reductase Biochemistry V. 41 11990 2002.
ISSN: ISSN 0006-2960
PubMed: 12356299
DOI: 10.1021/BI0203177