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Sodium in PDB 1lrl: Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose

Enzymatic activity of Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose

All present enzymatic activity of Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose:
5.1.3.2;

Protein crystallography data

The structure of Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose, PDB code: 1lrl was solved by J.B.Thoden, J.M.Henderson, J.L.Fridovich-Keil, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.80
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 83.200, 83.200, 109.500, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose (pdb code 1lrl). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose, PDB code: 1lrl:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 1lrl

Go back to Sodium Binding Sites List in 1lrl
Sodium binding site 1 out of 2 in the Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na342

b:21.4
occ:1.00
O A:HOH396 2.4 26.1 1.0
OE1 A:GLN91 2.4 37.6 1.0
O A:HOH565 2.5 46.4 1.0
O A:HOH430 2.6 32.4 1.0
O A:HOH566 2.8 62.0 1.0
O A:HOH564 3.0 52.5 1.0
CD A:GLN91 3.4 32.2 1.0
O A:HOH569 3.7 55.9 1.0
NE2 A:GLN91 3.7 30.1 1.0
CA A:GLN91 4.5 22.4 1.0
CG A:GLN91 4.6 18.1 1.0
O A:VAL90 4.7 24.7 1.0
CB A:GLN91 4.7 22.4 1.0
O A:GLN91 4.8 26.7 1.0

Sodium binding site 2 out of 2 in 1lrl

Go back to Sodium Binding Sites List in 1lrl
Sodium binding site 2 out of 2 in the Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-Glucose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na343

b:26.0
occ:1.00
O A:GLN334 2.3 37.3 1.0
O A:HOH501 2.4 52.7 1.0
O A:HOH483 2.4 41.3 1.0
O A:HOH382 2.6 33.0 1.0
C A:GLN334 3.5 38.1 1.0
O A:TYR336 3.7 29.9 1.0
NZ A:LYS37 4.0 29.6 1.0
NE2 A:GLN194 4.3 59.9 1.0
OD2 A:ASP192 4.3 29.4 1.0
CA A:GLN334 4.3 34.1 1.0
CA A:GLY335 4.3 25.9 1.0
N A:GLY335 4.3 41.9 1.0
OD1 A:ASP192 4.4 42.4 1.0
N A:TYR336 4.5 35.6 1.0
CB A:GLN334 4.6 52.2 1.0
C A:GLY335 4.6 42.1 1.0
OE1 A:GLN194 4.7 88.0 1.0
C A:TYR336 4.8 33.0 1.0
CG A:ASP192 4.8 24.8 1.0
O A:HOH632 4.8 47.6 1.0
CG A:GLN334 4.8 61.5 1.0
OD2 A:ASP338 4.9 72.9 1.0
CD A:GLN194 4.9 43.6 1.0

Reference:

J.B.Thoden, J.M.Henderson, J.L.Fridovich-Keil, H.M.Holden. Structural Analysis of the Y299C Mutant of Escherichia Coli Udp-Galactose 4-Epimerase. Teaching An Old Dog New Tricks. J.Biol.Chem. V. 277 27528 2002.
ISSN: ISSN 0021-9258
PubMed: 12019271
DOI: 10.1074/JBC.M204413200
Page generated: Tue Dec 15 05:29:31 2020

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