Sodium in PDB 1lrk: Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine

All present enzymatic activity of Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine:
5.1.3.2;

The structure of Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine, PDB code: 1lrk was solved by J.B.Thoden, J.M.Henderson, J.L.Fridovich-Keil, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.75
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	83.800, 83.800, 109.600, 90.00, 90.00, 120.00
R / Rfree (%)	n/a / n/a

The binding sites of Sodium atom in the Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine (pdb code 1lrk). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine, PDB code: 1lrk:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine within 5.0Å range: probe atom residue distance (Å) B Occ A:Na342 b:31.1 occ:1.00 OE1 A:GLN91 2.4 30.4 1.0 O A:HOH437 2.5 25.2 1.0 O A:HOH588 2.5 38.8 1.0 O A:HOH438 2.5 24.5 1.0 O A:HOH676 2.6 45.7 1.0 CD A:GLN91 3.3 32.3 1.0 NE2 A:GLN91 3.6 26.4 1.0 O A:HOH800 3.7 51.2 1.0 O A:HOH677 3.9 37.3 1.0 O A:HOH675 3.9 45.7 1.0 CA A:GLN91 4.5 22.7 1.0 CG A:GLN91 4.6 21.4 1.0 O A:VAL90 4.8 20.3 1.0 CB A:GLN91 4.8 25.4 1.0 O A:GLN91 4.9 26.6 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Escherichia Coli Udp-Galactose 4-Epimerase Mutant Y299C Complexed with Udp-N-Acetylglucosamine within 5.0Å range: probe atom residue distance (Å) B Occ A:Na343 b:32.7 occ:1.00 O A:HOH559 2.3 33.0 1.0 O A:GLN334 2.3 29.9 1.0 O A:HOH558 2.4 33.4 1.0 O A:HOH388 2.5 35.5 1.0 O A:HOH878 2.7 52.3 1.0 C A:GLN334 3.4 33.5 1.0 NZ A:LYS37 3.8 36.3 1.0 O A:HOH836 3.9 62.7 1.0 O A:TYR336 4.0 32.7 1.0 CA A:GLN334 4.3 26.8 1.0 N A:GLY335 4.3 30.5 1.0 CA A:GLY335 4.3 24.1 1.0 CB A:GLN334 4.4 33.5 1.0 O A:HOH766 4.4 52.4 1.0 OD1 A:ASP192 4.5 28.1 1.0 C A:GLY335 4.6 34.3 1.0 NE2 A:GLN194 4.6 42.2 1.0 OE1 A:GLN194 4.6 77.7 1.0 OD2 A:ASP192 4.6 26.6 1.0 N A:TYR336 4.7 24.1 1.0 NE2 A:GLN334 4.8 0.0 1.0 O A:HOH657 4.8 40.6 1.0 OD2 A:ASP338 4.8 53.0 1.0 C A:TYR336 5.0 29.6 1.0

J.B.Thoden, J.M.Henderson, J.L.Fridovich-Keil, H.M.Holden. Structural Analysis of the Y299C Mutant of Escherichia Coli Udp-Galactose 4-Epimerase. Teaching An Old Dog New Tricks. J.Biol.Chem. V. 277 27528 2002.
ISSN: ISSN 0021-9258
PubMed: 12019271
DOI: 10.1074/JBC.M204413200