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Sodium in PDB 1lrj: Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine

Enzymatic activity of Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine

All present enzymatic activity of Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine:
5.1.3.2;

Protein crystallography data

The structure of Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine, PDB code: 1lrj was solved by J.B.Thoden, J.M.Henderson, J.L.Fridovich-Keil, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.90
Space group P 32 2 1
Cell size a, b, c (Å), α, β, γ (°) 83.200, 83.200, 108.300, 90.00, 90.00, 120.00
R / Rfree (%) n/a / n/a

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine (pdb code 1lrj). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine, PDB code: 1lrj:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in 1lrj

Go back to Sodium Binding Sites List in 1lrj
Sodium binding site 1 out of 2 in the Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na343

b:32.1
occ:1.00
O A:GLN334 2.4 45.4 1.0
O A:HOH566 2.5 36.3 1.0
O A:HOH622 2.5 38.4 1.0
O A:HOH481 2.6 55.7 1.0
O A:HOH683 2.6 41.2 1.0
O A:HOH736 3.2 64.4 1.0
C A:GLN334 3.6 30.9 1.0
O A:TYR336 3.6 30.5 1.0
NZ A:LYS37 3.7 34.9 1.0
OD2 A:ASP192 4.3 32.7 1.0
O A:HOH579 4.3 57.9 1.0
NE2 A:GLN194 4.3 50.1 1.0
CA A:GLY335 4.4 30.1 1.0
OE1 A:GLN194 4.4 0.0 1.0
N A:TYR336 4.4 30.7 1.0
N A:GLY335 4.4 28.6 1.0
C A:GLY335 4.5 33.0 1.0
CA A:GLN334 4.5 26.0 1.0
OD1 A:ASP192 4.6 22.0 1.0
C A:TYR336 4.6 27.9 1.0
CE A:LYS37 4.8 37.2 1.0
CD A:GLN194 4.8 42.2 1.0
CB A:GLN334 4.8 36.6 1.0
CG A:ASP192 4.9 34.5 1.0
NE2 A:GLN334 5.0 0.0 1.0

Sodium binding site 2 out of 2 in 1lrj

Go back to Sodium Binding Sites List in 1lrj
Sodium binding site 2 out of 2 in the Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of E. Coli Udp-Galactose 4-Epimerase Complexed with Udp-N-Acetylglucosamine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na344

b:41.7
occ:1.00
OE1 A:GLN91 2.4 53.4 1.0
O A:HOH757 2.5 56.1 1.0
O A:HOH536 2.6 36.8 1.0
CD A:GLN91 3.2 51.1 1.0
O A:HOH694 3.2 83.8 1.0
NE2 A:GLN91 3.5 37.0 1.0
O A:HOH656 3.7 61.9 1.0
O A:HOH629 4.0 52.2 1.0
CG A:GLN91 4.6 34.9 1.0
O A:HOH747 4.7 64.7 1.0
CA A:GLN91 4.7 23.2 1.0
CB A:GLN91 4.9 28.4 1.0
O A:VAL90 4.9 25.8 1.0

Reference:

J.B.Thoden, J.M.Henderson, J.L.Fridovich-Keil, H.M.Holden. Structural Analysis of the Y299C Mutant of Escherichia Coli Udp-Galactose 4-Epimerase. Teaching An Old Dog New Tricks. J.Biol.Chem. V. 277 27528 2002.
ISSN: ISSN 0021-9258
PubMed: 12019271
DOI: 10.1074/JBC.M204413200
Page generated: Sun Oct 6 20:17:50 2024

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