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Sodium in PDB 1c7p: Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal

Enzymatic activity of Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal

All present enzymatic activity of Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal:
3.2.1.17;

Protein crystallography data

The structure of Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal, PDB code: 1c7p was solved by S.Goda, K.Takano, Y.Yamagata, Y.Katakura, K.Yutani, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 5.00 / 2.40
Space group P 61 2 2
Cell size a, b, c (Å), α, β, γ (°) 91.080, 91.080, 92.730, 90.00, 90.00, 120.00
R / Rfree (%) 19.7 / n/a

Sodium Binding Sites:

The binding sites of Sodium atom in the Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal (pdb code 1c7p). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 3 binding sites of Sodium where determined in the Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal, PDB code: 1c7p:
Jump to Sodium binding site number: 1; 2; 3;

Sodium binding site 1 out of 3 in 1c7p

Go back to Sodium Binding Sites List in 1c7p
Sodium binding site 1 out of 3 in the Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na901

b:6.9
occ:1.00
NA A:NA903 3.0 2.0 1.0
CD A:GLU4 4.8 30.5 1.0
OE2 A:GLU4 4.9 39.3 1.0

Sodium binding site 2 out of 3 in 1c7p

Go back to Sodium Binding Sites List in 1c7p
Sodium binding site 2 out of 3 in the Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na902

b:2.0
occ:1.00
O A:HOH831 2.1 11.3 1.0
OE2 A:GLU7 2.1 23.4 1.0
CD A:GLU7 3.2 22.7 1.0
OE1 A:GLU7 3.5 26.2 1.0
OG1 A:THR11 4.3 11.6 1.0
CG A:GLU7 4.5 18.2 1.0
CB A:ARG10 4.8 10.4 1.0
NE A:ARG10 4.8 7.7 1.0
NE A:ARG14 4.9 22.9 1.0
O A:GLU7 4.9 11.3 1.0
O A:HOH617 5.0 33.1 1.0

Sodium binding site 3 out of 3 in 1c7p

Go back to Sodium Binding Sites List in 1c7p
Sodium binding site 3 out of 3 in the Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 3 of Crystal Structure of Mutant Human Lysozyme with Four Extra Residues (Eaea) at the N-Terminal within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Na903

b:2.0
occ:1.00
NA A:NA901 3.0 6.9 1.0
OE1 A:GLU4 3.2 31.9 1.0
CD A:GLU4 3.5 30.5 1.0
CG A:GLU4 3.8 24.5 1.0
OE2 A:GLU4 4.3 39.3 1.0
O A:HOH131 4.7 25.3 1.0
O A:HOH217 4.7 17.7 1.0
CB A:GLU4 4.9 16.0 1.0
O A:HOH325 4.9 36.3 1.0

Reference:

S.Goda, K.Takano, Y.Yamagata, Y.Katakura, K.Yutani. Effect of Extra N-Terminal Residues on the Stability and Folding of Human Lysozyme Expressed in Pichia Pastoris. Protein Eng. V. 13 299 2000.
ISSN: ISSN 0269-2139
PubMed: 10810162
DOI: 10.1093/PROTEIN/13.4.299
Page generated: Tue Dec 15 05:22:32 2020

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