Sodium in PDB 7o86: 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak

All present enzymatic activity of 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak:
2.7.11.1;

The structure of 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak, PDB code: 7o86 was solved by K.T.Elvers, B.D.Bax, M.Lipka-Lloyd, Y.Mehellou, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.45 / 1.73
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	39.611, 50.549, 103.798, 90, 90, 90
R / Rfree (%)	19.9 / 24

The structure of 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Calcium	(Ca)	1 atom

The binding sites of Sodium atom in the 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak (pdb code 7o86). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak, PDB code: 7o86:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak within 5.0Å range: probe atom residue distance (Å) B Occ A:Na601 b:46.7 occ:1.00 O B:HOH738 2.1 39.4 1.0 O A:HOH734 2.3 24.0 1.0 O B:HOH757 2.7 28.3 1.0 O A:HOH740 2.7 22.7 1.0 O B:HOH789 3.2 49.3 1.0 O A:HIS495 3.6 25.4 1.0 CG2 A:VAL498 3.9 21.6 1.0 C A:HIS495 4.1 26.6 1.0 CA A:HIS495 4.2 21.4 1.0 CB B:PHE519 4.3 21.3 1.0 O A:GLY494 4.3 22.1 1.0 CG1 A:VAL498 4.4 23.3 1.0 CG2 B:ILE499 4.6 19.7 1.0 N B:LYS520 4.7 21.0 0.5 N B:LYS520 4.7 21.0 0.5 O A:HOH802 4.7 44.2 1.0 O B:LYS520 4.8 18.5 0.5 O B:LYS520 4.8 18.5 0.5 ND2 B:ASN503 4.8 24.5 1.0 CB A:VAL498 4.8 22.2 1.0 ND1 A:HIS495 4.9 21.7 1.0 CA B:PHE519 4.9 21.7 1.0 CB A:HIS495 4.9 21.3 1.0

Sodium binding site 2 out of 2 in the 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of 1.73A X-Ray Crystal Structure of the Conserved C-Terminal (Cct) of Human Spak within 5.0Å range: probe atom residue distance (Å) B Occ A:Na602 b:28.3 occ:0.50 O A:PRO474 2.2 34.2 0.5 O A:ASP477 2.5 28.3 1.0 N A:GLY475 3.1 29.4 0.5 C A:PRO474 3.3 30.4 0.5 C A:ASP477 3.7 29.9 1.0 CA A:GLY475 3.9 30.1 0.5 CA A:PRO474 4.0 28.6 0.5 CA A:PRO474 4.0 28.6 0.5 C A:PRO474 4.0 31.7 0.5 N A:ASP477 4.2 23.6 1.0 C A:GLY475 4.2 28.8 0.5 N A:GLY475 4.3 31.0 0.5 C A:GLY475 4.4 28.5 0.5 O A:THR473 4.4 30.6 1.0 CE1 A:PHE472 4.4 28.8 1.0 N A:ARG476 4.4 29.3 1.0 CA A:GLY475 4.5 29.2 0.5 CZ A:PHE472 4.5 28.5 1.0 CA A:ASP477 4.5 23.8 1.0 N A:THR478 4.6 25.1 1.0 CA A:THR478 4.7 23.9 1.0 C A:ARG476 4.7 25.7 1.0 O A:GLY475 4.8 29.6 0.5 O A:GLY475 4.9 29.3 0.5 CG2 A:THR478 4.9 27.9 1.0 CB A:PRO474 4.9 34.1 0.5

K.T.Elvers, M.Lipka-Lloyd, B.D.Bax, Y.Mehellou. Crystal Structure of the Human Spak C-Terminal Domain To Be Published.