Sodium in PDB 7sqi: Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp

All present enzymatic activity of Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp:
2.3.1.41;

The structure of Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp, PDB code: 7sqi was solved by A.Chen, J.T.Mindrebo, T.D.Davis, J.P.Noel, M.D.Burkart, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.10 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	58.99, 112.38, 141.64, 90, 90, 90
R / Rfree (%)	17.1 / 20.5

The binding sites of Sodium atom in the Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp (pdb code 7sqi). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp, PDB code: 7sqi:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp within 5.0Å range: probe atom residue distance (Å) B Occ A:Na501 b:15.7 occ:1.00 OD1 A:ASN296 2.4 17.6 1.0 OE1 A:GLU342 2.4 21.9 1.0 OG A:SER387 2.6 12.8 1.0 O A:ASN296 2.6 13.2 1.0 O A:ASN388 2.6 13.1 1.0 O A:SER297 3.2 16.9 1.0 C A:ASN296 3.4 14.4 1.0 CG A:ASN296 3.4 16.6 1.0 CD A:GLU342 3.4 24.8 1.0 N A:ASN388 3.4 13.2 1.0 C A:SER297 3.6 15.9 1.0 C A:ASN388 3.6 13.1 1.0 CB A:GLU342 3.7 13.9 1.0 CB A:SER387 3.8 14.2 1.0 CB A:ASN296 3.8 14.2 1.0 O A:HOH612 3.9 25.0 1.0 CG A:GLU342 4.0 18.5 1.0 C A:SER387 4.0 12.3 1.0 CA A:SER387 4.0 13.5 1.0 N A:HIS298 4.0 15.1 1.0 O A:HOH750 4.1 18.6 1.0 CA A:ASN388 4.1 12.9 1.0 N A:SER297 4.1 12.8 1.0 CA A:HIS298 4.2 15.8 1.0 CA A:ASN296 4.2 11.7 1.0 CA A:SER297 4.3 14.1 1.0 OE2 A:GLU342 4.5 23.9 1.0 O A:HOH654 4.5 15.5 1.0 ND2 A:ASN296 4.6 17.4 1.0 OG A:SER389 4.7 16.6 1.0 N A:SER389 4.8 10.7 1.0 CB A:ASN388 4.8 12.7 1.0 O A:SER387 5.0 11.5 1.0

Sodium binding site 2 out of 2 in the Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, Fabb, and C14-Crypto Acyl Carrier Protein, Acpp within 5.0Å range: probe atom residue distance (Å) B Occ B:Na501 b:16.5 occ:1.00 OE1 B:GLU342 2.3 20.6 1.0 OD1 B:ASN296 2.3 17.0 1.0 O B:ASN296 2.6 14.9 1.0 OG B:SER387 2.6 16.2 1.0 O B:ASN388 2.6 13.7 1.0 O B:SER297 3.2 15.9 1.0 CD B:GLU342 3.3 29.0 1.0 C B:ASN296 3.4 14.9 1.0 CG B:ASN296 3.4 14.4 1.0 N B:ASN388 3.5 13.9 1.0 C B:SER297 3.6 16.1 1.0 C B:ASN388 3.6 12.9 1.0 CB B:GLU342 3.7 13.6 1.0 CB B:SER387 3.8 12.3 1.0 CB B:ASN296 3.8 12.4 1.0 CG B:GLU342 3.9 16.9 1.0 CA B:SER387 4.0 11.8 1.0 N B:HIS298 4.0 14.5 1.0 C B:SER387 4.0 10.7 1.0 O B:HOH699 4.0 19.6 1.0 CA B:ASN388 4.1 13.2 1.0 N B:SER297 4.1 15.7 1.0 CA B:HIS298 4.2 14.8 1.0 CA B:ASN296 4.2 13.5 1.0 CA B:SER297 4.3 14.5 1.0 OE2 B:GLU342 4.3 26.9 1.0 O B:HOH650 4.5 15.2 1.0 ND2 B:ASN296 4.6 17.2 1.0 OG B:SER389 4.7 17.0 1.0 CB B:ASN388 4.7 14.3 1.0 N B:SER389 4.8 13.8 1.0 O B:SER387 5.0 13.1 1.0

A.Chen, J.T.Mindrebo, T.D.Davis, W.E.Kim, Y.Katsuyama, Z.Jiang, Y.Ohnishi, J.P.Noel, M.D.Burkart. Mechanism-Based Cross-Linking Probes Capture the Escherichia Coli Ketosynthase Fabb in Conformationally Distinct Catalytic States. Acta Crystallogr D Struct V. 78 1171 2022BIOL.
ISSN: ISSN 2059-7983
PubMed: 36048156
DOI: 10.1107/S2059798322007434