Sodium in PDB 7pu0: Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus

All present enzymatic activity of Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus:
1.10.3.2;

The structure of Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus, PDB code: 7pu0 was solved by A.Gabdulkhakov, S.Tishchenko, I.Kolyadenko, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.74 / 2.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	76.235, 94.591, 120.867, 90, 91.39, 90
R / Rfree (%)	15.7 / 18.9

The structure of Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus also contains other interesting chemical elements:

Copper

(Cu)

24 atoms

The binding sites of Sodium atom in the Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus (pdb code 7pu0). This binding sites where shown within 5.0 Angstroms radius around Sodium atom.
In total 2 binding sites of Sodium where determined in the Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus, PDB code: 7pu0:
Jump to Sodium binding site number: 1; 2;

Sodium binding site 1 out of 2 in the Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 1 of Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus within 5.0Å range: probe atom residue distance (Å) B Occ C:Na405 b:41.7 occ:1.00 O C:HOH515 2.4 34.4 1.0 O A:HOH565 2.4 29.9 1.0 O B:HOH545 2.4 34.0 1.0 O B:HOH552 2.5 23.1 1.0 O C:HOH519 2.6 23.4 1.0 O A:HOH517 2.8 23.4 1.0 O C:HOH535 3.6 19.6 1.0 O A:HOH560 3.7 22.3 1.0 O B:HOH582 3.8 18.6 1.0 O C:ASP243 4.2 19.9 1.0 O B:ASP243 4.2 21.7 1.0 OG C:SER256 4.4 20.7 1.0 O A:ASP243 4.6 20.2 1.0 OG B:SER256 4.7 16.1 1.0 OG A:SER256 4.8 16.9 1.0 CB C:SER256 4.9 14.3 1.0 CA C:SER256 4.9 15.3 1.0

Sodium binding site 2 out of 2 in the Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus


Mono view


Stereo pair view

A full contact list of Sodium with other atoms in the Na binding site number 2 of Crystal Structure of Two-Domain Laccase Mutant H165A/M199G From Streptomyces Griseoflavus within 5.0Å range: probe atom residue distance (Å) B Occ D:Na405 b:46.4 occ:1.00 O D:HOH502 2.2 41.1 1.0 O D:HOH555 2.3 25.6 1.0 O F:HOH560 2.5 22.0 1.0 O F:HOH530 2.5 31.1 1.0 O E:HOH523 2.8 20.0 1.0 O E:HOH503 2.8 26.7 1.0 O D:HOH567 3.5 28.1 1.0 O E:HOH560 3.6 20.8 1.0 O F:HOH532 4.1 21.1 1.0 O D:ASP243 4.2 21.0 1.0 O F:ASP243 4.3 17.3 1.0 OG D:SER256 4.6 18.6 1.0 O E:ASP243 4.6 21.5 1.0 OG E:SER256 4.8 17.2 1.0 OG F:SER256 4.9 16.6 1.0 CA D:SER256 4.9 20.7 1.0 CB D:SER256 5.0 15.0 1.0

I.Kolyadenko, A.Scherbakova, K.Kovalev, A.Gabdulkhakov, S.Tishchenko. Engineering the Catalytic Properties of Two-Domain Laccase From Streptomyces Griseoflavus Ac-993. Int J Mol Sci V. 23 2021.
ISSN: ESSN 1422-0067
PubMed: 35008493
DOI: 10.3390/IJMS23010065